Glucose 6 Phosphate Dehydrogenase Activity Assay Kit (Fluorometric) (ab176722)
- Datasheet
- References
- Protocols
Overview
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Product name
Glucose 6 Phosphate Dehydrogenase Activity Assay Kit (Fluorometric)
See all Glucose 6 Phosphate Dehydrogenase kits -
Detection method
Fluorescent -
Sample type
Urine, Serum, Plasma, Other biological fluids, Whole Blood, Cell Lysate -
Assay type
Enzyme activity (quantitative) -
Sensitivity
>= 0.3 mIU/ml -
Assay time
1h 00m -
Species reactivity
Reacts with: Other species, Mammals -
Product overview
Glucose 6 Phosphate Dehydrogenase Activity Assay Kit (Fluorometric) (ab176722) provides a simple method for detecting glucose 6 phosphate dehydrogenase (G6PD) activity in a variety of biological samples such as serum, plasma and urine, as well as cell extracts. In the assay, G6PD present in the sample converts NADP+ to NADPH, which can be specifically monitored by a fluorogenic NADPH sensor to yield a highly red fluorescence product. The signal can be easily detected at Ex/Em = 540/590 nm in a fluorescent microplate reader. The fluorescence intensity is proportional to the G6PD activity present in the sample.
The assay can detect glucose 6 phosphate dehydrogenase activity from as low as 0.3 mU/mL in a 100 µL reaction volume.
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Notes
Glucose 6 Phosphate Dehydrogenase (G6PD, G6PDH, EC 1.1.1.49) catalyzes the conversion of glucose-6-phosphate to 6-phosphoglucono-δ-lactone, the first and rate-limiting step in the pentose phosphate pathway (PPP). It is a critical metabolic pathway that supplies reducing energy to cells (such as erythrocytes) by maintaining the level of co-enzyme nicotinamide adenine dinucleotide phosphate (NADPH). The production of NADPH is of great importance for tissues actively engaged in biosynthesis of fatty acids and/or isoprenoids, such as the liver, mammary glands, adipose tissue, and the adrenal glands. The NADPH also maintains the level of glutathione in these cells that helps protect the red blood cells against oxidative damage. Deficiencies in G6PD predispose individuals to non-immune hemolytic anemia.
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Platform
Microplate reader
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 200 tests Assay Buffer 1 x 10ml Enzyme Probe 2 vials G6PD Standard 1 vial NADP 1 vial -
Research areas
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Relevance
Glucose 6 phosphate dehydrogenase (G6PDH) is a cytosolic enzyme in the pentose phosphate pathway, a metabolic pathway that supplies reducing energy to cells (such as erythrocytes) by maintaining the level of the co-enzyme nicotinamide adenine dinucleotide phosphate (NADPH). The NADPH in turn maintains the level of glutathione in these cells that helps protect the red blood cells against oxidative damage. Of greater quantitative importance is the production of NADPH for tissues actively engaged in biosynthesis of fatty acids and/or isoprenoids, such as the liver, mammary glands, adipose tissue, and the adrenal glands. -
Alternative names
- EC=1.1.1.49
- G6PD
- G6PD1
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Images
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Glucose 6 Phosphate Dehydrogenase Dose response CurveG6PD was measured with Abcam's Glucose 6 Phosphate Dehydrogenase Assay Kit (Fluorometric) (ab176722) in a 96-well black plate using a microplate reader. As low as 0.3 mU/mL Glucose 6 Phosphate Dehydrogenase in 100 µL volume can be detected with 1 hour incubation.
Protocols
References
ab176722 has not yet been referenced specifically in any publications.
