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Signal Transduction Metabolism Energy Metabolism
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Glucose 6 Phosphate Dehydrogenase Activity Assay Kit (Fluorometric) (ab176722)

  • Datasheet
  • SDS
  • Protocol Booklet
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Glucose 6 Phosphate Dehydrogenase Dose response Curve

    Key features and details

    • Assay type: Enzyme activity (quantitative)
    • Detection method: Fluorescent
    • Platform: Microplate reader
    • Assay time: 1 hr
    • Sample type: Cell Lysate, Other biological fluids, Plasma, Serum, Urine, Whole Blood
    • Sensitivity: 0.3 mIU/ml

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    Overview

    • Product name

      Glucose 6 Phosphate Dehydrogenase Activity Assay Kit (Fluorometric)
      See all Glucose 6 Phosphate Dehydrogenase kits
    • Detection method

      Fluorescent
    • Sample type

      Urine, Serum, Plasma, Other biological fluids, Whole Blood, Cell Lysate
    • Assay type

      Enzyme activity (quantitative)
    • Sensitivity

      >= 0.3 mIU/ml
    • Assay time

      1h 00m
    • Species reactivity

      Reacts with: Mammals, Other species
    • Product overview

      Glucose 6 Phosphate Dehydrogenase Activity Assay Kit (Fluorometric) (ab176722) provides a simple method for detecting glucose 6 phosphate dehydrogenase (G6PD) activity in a variety of biological samples such as serum, plasma and urine, as well as cell extracts. In the assay, G6PD present in the sample converts NADP+ to NADPH, which can be specifically monitored by a fluorogenic NADPH sensor to yield a highly red fluorescence product. The signal can be easily detected at Ex/Em = 540/590 nm in a fluorescent microplate reader. The fluorescence intensity is proportional to the G6PD activity present in the sample.


      The assay can detect glucose 6 phosphate dehydrogenase activity from as low as 0.3 mU/mL in a 100 µL reaction volume.

    • Notes

      Glucose 6 Phosphate Dehydrogenase (G6PD, G6PDH, EC 1.1.1.49) catalyzes the conversion of glucose-6-phosphate to 6-phosphoglucono-δ-lactone, the first and rate-limiting step in the pentose phosphate pathway (PPP). It is a critical metabolic pathway that supplies reducing energy to cells (such as erythrocytes) by maintaining the level of co-enzyme nicotinamide adenine dinucleotide phosphate (NADPH). The production of NADPH is of great importance for tissues actively engaged in biosynthesis of fatty acids and/or isoprenoids, such as the liver, mammary glands, adipose tissue, and the adrenal glands. The NADPH also maintains the level of glutathione in these cells that helps protect the red blood cells against oxidative damage. Deficiencies in G6PD predispose individuals to non-immune hemolytic anemia.

    • Platform

      Microplate reader

    Properties

    • Storage instructions

      Store at -20°C. Please refer to protocols.
    • Components 200 tests
      Assay Buffer 1 x 10ml
      Enzyme Probe 2 vials
      G6PD Standard 1 vial
      NADP 1 vial
    • Research areas

      • Signal Transduction
      • Metabolism
      • Energy Metabolism
      • Cancer
      • Cancer Metabolism
      • Metabolic signaling pathway
      • Metabolism of carbohydrates
      • Metabolism
      • Pathways and Processes
      • Metabolic signaling pathways
      • Carbohydrate metabolism
      • Metabolism
      • Pathways and Processes
      • Metabolic signaling pathways
      • Energy transfer pathways
      • Energy Metabolism
    • Relevance

      Glucose 6 phosphate dehydrogenase (G6PDH) is a cytosolic enzyme in the pentose phosphate pathway, a metabolic pathway that supplies reducing energy to cells (such as erythrocytes) by maintaining the level of the co-enzyme nicotinamide adenine dinucleotide phosphate (NADPH). The NADPH in turn maintains the level of glutathione in these cells that helps protect the red blood cells against oxidative damage. Of greater quantitative importance is the production of NADPH for tissues actively engaged in biosynthesis of fatty acids and/or isoprenoids, such as the liver, mammary glands, adipose tissue, and the adrenal glands.
    • Alternative names

      • EC=1.1.1.49
      • G6PD
      • G6PD1
      • G6PDH
      • Glucose 6 phosphate 1 dehydrogenase
      see all

    Associated products

      Images

      • Glucose 6 Phosphate Dehydrogenase Dose response Curve
        Glucose 6 Phosphate Dehydrogenase Dose response Curve

        G6PD was measured with Abcam's Glucose 6 Phosphate Dehydrogenase Assay Kit (Fluorometric) (ab176722) in a 96-well black plate using a microplate reader. As low as 0.3 mU/mL Glucose 6 Phosphate Dehydrogenase in 100 µL volume can be detected with 1 hour incubation.

        The concentration of the Glucose-6-Phosphate Dehydrogenase in X-axis is based on the 50 uL standard, the final in-well concentration should be 1/2 for each point.

      Protocols

      • Protocol Booklet

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet
      • SDS
    • References (0)

      Publishing research using ab176722? Please let us know so that we can cite the reference in this datasheet.

      ab176722 has not yet been referenced specifically in any publications.

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