Anti-Glucose 6 Phosphate Dehydrogenase antibody (ab226964)
Key features and details
- Rabbit polyclonal to Glucose 6 Phosphate Dehydrogenase
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
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- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-Glucose 6 Phosphate Dehydrogenase antibody
See all Glucose 6 Phosphate Dehydrogenase primary antibodies -
Description
Rabbit polyclonal to Glucose 6 Phosphate Dehydrogenase -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Sheep, Rabbit, Cow, Rhesus monkey -
Immunogen
Recombinant fragment within Human Glucose 6 Phosphate Dehydrogenase (internal sequence). The exact sequence is proprietary.
Database link: P11413 -
Positive control
- WB: Neuro2a, PC-12, Rat2, HeLa S3, C8D30, NIH/3T3, RAW 264.7 and C1C12 whole cell extracts. IHC-P: U87 xenograft tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab226964 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/3000. Predicted molecular weight: 59 kDa.
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IHC-P |
1/100 - 1/1000.
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Notes |
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WB
1/500 - 1/3000. Predicted molecular weight: 59 kDa. |
IHC-P
1/100 - 1/1000. |
Target
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Function
Catalyzes the rate-limiting step of the oxidative pentose-phosphate pathway, which represents a route for the dissimilation of carbohydrates besides glycolysis. The main function of this enzyme is to provide reducing power (NADPH) and pentose phosphates for fatty acid and nucleic acid synthesis. -
Tissue specificity
Isoform Long is found in lymphoblasts, granulocytes and sperm. -
Pathway
Carbohydrate degradation; pentose phosphate pathway; D-ribulose 5-phosphate from D-glucose 6-phosphate (oxidative stage): step 1/3. -
Involvement in disease
Anemia, non-spherocytic hemolytic, due to G6PD deficiency -
Sequence similarities
Belongs to the glucose-6-phosphate dehydrogenase family. -
Post-translational
modificationsAcetylated by ELP3 at Lys-403; acetylation inhibits its homodimerization and enzyme activity. Deacetylated by SIRT2 at Lys-403; deacetylation stimulates its enzyme activity. - Information by UniProt
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Database links
- Entrez Gene: 281179 Cow
- Entrez Gene: 2539 Human
- Entrez Gene: 14381 Mouse
- Entrez Gene: 24377 Rat
- Entrez Gene: 443211 Sheep
- Omim: 305900 Human
- SwissProt: P11413 Human
- SwissProt: Q00612 Mouse
see all -
Alternative names
- G6PD antibody
- G6PD_HUMAN antibody
- G6PD1 antibody
see all
Images
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All lanes : Anti-Glucose 6 Phosphate Dehydrogenase antibody (ab226964) at 1/500 dilution
Lane 1 : Neuro-2a (mouse neuroblastoma cell line) whole cell extracts
Lane 2 : C8D30 whole cell extracts
Lane 3 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell extracts
Lane 4 : RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell extracts
Lane 5 : C2C12 (mouse myoblast cell line) whole cell extracts
Lysates/proteins at 30 µg per lane.
Developed using the ECL technique.
Predicted band size: 59 kDa7.5% SDS-PAGE
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Paraffin-embedded U87 xenograft tissue stained for Glucose 6 Phosphate Dehydrogenase with ab226964 (1/300 dilution) in immunohistochemical analysis.
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All lanes : Anti-Glucose 6 Phosphate Dehydrogenase antibody (ab226964) at 1/500 dilution
Lane 1 : PC-12 (rat adrenal gland pheochromocytoma cell line) whole cell extracts
Lane 2 : Rat2 (rat fibroblast cell line) whole cell extracts
Lysates/proteins at 30 µg per lane.
Developed using the ECL technique.
Predicted band size: 59 kDa7.5% SDS-PAGE
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Anti-Glucose 6 Phosphate Dehydrogenase antibody (ab226964) at 1/500 dilution + HeLa S3 (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 30 µg
Developed using the ECL technique.
Predicted band size: 59 kDa7.5% SDS PAGE
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab226964 has not yet been referenced specifically in any publications.