Overview

  • Product name

    Anti-Glucose 6 Phosphate Dehydrogenase antibody
    See all Glucose 6 Phosphate Dehydrogenase primary antibodies
  • Description

    Rabbit polyclonal to Glucose 6 Phosphate Dehydrogenase
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
    Predicted to work with: Sheep, Rabbit, Cow, Rhesus monkey
  • Immunogen

    Recombinant fragment within Human Glucose 6 Phosphate Dehydrogenase (internal sequence). The exact sequence is proprietary.
    Database link: P11413

  • Positive control

    • WB: Neuro2a, PC-12, Rat2, HeLa S3, C8D30, NIH/3T3, RAW 264.7 and C1C12 whole cell extracts. IHC-P: U87 xenograft tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab226964 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 59 kDa.
IHC-P 1/100 - 1/1000.

Target

  • Function

    Catalyzes the rate-limiting step of the oxidative pentose-phosphate pathway, which represents a route for the dissimilation of carbohydrates besides glycolysis. The main function of this enzyme is to provide reducing power (NADPH) and pentose phosphates for fatty acid and nucleic acid synthesis.
  • Tissue specificity

    Isoform Long is found in lymphoblasts, granulocytes and sperm.
  • Pathway

    Carbohydrate degradation; pentose phosphate pathway; D-ribulose 5-phosphate from D-glucose 6-phosphate (oxidative stage): step 1/3.
  • Involvement in disease

    Anemia, non-spherocytic hemolytic, due to G6PD deficiency
  • Sequence similarities

    Belongs to the glucose-6-phosphate dehydrogenase family.
  • Post-translational
    modifications

    Acetylated by ELP3 at Lys-403; acetylation inhibits its homodimerization and enzyme activity. Deacetylated by SIRT2 at Lys-403; deacetylation stimulates its enzyme activity.
  • Information by UniProt
  • Database links

  • Alternative names

    • G6PD antibody
    • G6PD_HUMAN antibody
    • G6PD1 antibody
    • G6pdx antibody
    • Glucose 6 phosphate 1 dehydrogenase antibody
    • Glucose 6 phosphate dehydrogenase antibody
    • Glucose 6 phosphate dehydrogenase, G6PD antibody
    • Glucose-6-phosphate 1-dehydrogenase antibody
    • MET19 antibody
    • POS10 antibody
    • Zwf1p antibody
    see all

Images

  • All lanes : Anti-Glucose 6 Phosphate Dehydrogenase antibody (ab226964) at 1/500 dilution

    Lane 1 : Neuro-2a (mouse neuroblastoma cell line) whole cell extracts
    Lane 2 : C8D30 whole cell extracts
    Lane 3 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell extracts
    Lane 4 : RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell extracts
    Lane 5 : C2C12 (mouse myoblast cell line) whole cell extracts

    Lysates/proteins at 30 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 59 kDa



    7.5% SDS-PAGE

  • Paraffin-embedded U87 xenograft tissue stained for Glucose 6 Phosphate Dehydrogenase with ab226964 (1/300 dilution) in immunohistochemical analysis.

  • All lanes : Anti-Glucose 6 Phosphate Dehydrogenase antibody (ab226964) at 1/500 dilution

    Lane 1 : PC-12 (rat adrenal gland pheochromocytoma cell line) whole cell extracts
    Lane 2 : Rat2 (rat fibroblast cell line) whole cell extracts

    Lysates/proteins at 30 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 59 kDa



    7.5% SDS-PAGE

  • Anti-Glucose 6 Phosphate Dehydrogenase antibody (ab226964) at 1/500 dilution + HeLa S3 (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 30 µg

    Developed using the ECL technique.

    Predicted band size: 59 kDa



    7.5% SDS PAGE

References

ab226964 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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