Product nameAnti-Glucose 6 Phosphate Dehydrogenase antibody
See all Glucose 6 Phosphate Dehydrogenase primary antibodies
DescriptionRabbit polyclonal to Glucose 6 Phosphate Dehydrogenase
Tested applicationsSuitable for: ICC/IF, IHC-P, WBmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Sheep, Cow, Chimpanzee, Baboon, Chinese hamster
Synthetic peptide corresponding to Human Glucose 6 Phosphate Dehydrogenase aa 50-150.
(Peptide available as
- Recombinant human Glucose 6 Phosphate Dehydrogenase protein (ab126671) can be used as a positive control in WB. This antibody gave a positive signal in RAW 264.7 and Jurkat whole cell lysates, and in human placenta and lymph node tissue lysates. IHC-P: Hodgkin's lymphoma
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
PurityImmunogen affinity purified
- Pathways and Processes
- Metabolic signaling pathways
- Energy transfer pathways
- Energy Metabolism
Our Abpromise guarantee covers the use of ab76598 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 1 µg/ml.|
|IHC-P||Use a concentration of 0.2 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 59 kDa (predicted molecular weight: 59 kDa).|
FunctionCatalyzes the rate-limiting step of the oxidative pentose-phosphate pathway, which represents a route for the dissimilation of carbohydrates besides glycolysis. The main function of this enzyme is to provide reducing power (NADPH) and pentose phosphates for fatty acid and nucleic acid synthesis.
Tissue specificityIsoform Long is found in lymphoblasts, granulocytes and sperm.
PathwayCarbohydrate degradation; pentose phosphate pathway; D-ribulose 5-phosphate from D-glucose 6-phosphate (oxidative stage): step 1/3.
Involvement in diseaseAnemia, non-spherocytic hemolytic, due to G6PD deficiency
Sequence similaritiesBelongs to the glucose-6-phosphate dehydrogenase family.
modificationsAcetylated by ELP3 at Lys-403; acetylation inhibits its homodimerization and enzyme activity. Deacetylated by SIRT2 at Lys-403; deacetylation stimulates its enzyme activity.
- Information by UniProt
- G6PD antibody
- G6PD_HUMAN antibody
- G6PD1 antibody
All lanes : Anti-Glucose 6 Phosphate Dehydrogenase antibody (ab76598) at 1 µg/ml
Lane 1 : RAW 264.7 (Mouse leukaemic monocyte macrophage cell line) Whole Cell Lysate
Lane 2 : Human placenta tissue lysate - total protein (ab29745)
Lane 3 : Human lymph node tissue lysate - total protein (ab29871)
Lane 4 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 59 kDa
Observed band size: 59 kDa
Exposure time: 30 seconds
ICC/IF image of ab76598 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab76598, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 100% methanol fixed (5 min) Hek293, HepG2 and MCF7 cells at 1µg/ml.
IHC image of Glucose 6 Phosphate staining in human Hodgkin's Lymphoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab76598, 0.2µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
This product has been referenced in:
- Chhabra A et al. Glucose-6-phosphate dehydrogenase is critical for suppression of cardiac hypertrophy by H2S. Cell Death Discov 4:6 (2018). Read more (PubMed: 29531803) »
- Choudhary I et al. Proteomic Investigation to Identify Anticancer Targets of Nemopilema nomurai Jellyfish Venom in Human Hepatocarcinoma HepG2 Cells. Toxins (Basel) 10:N/A (2018). Read more (PubMed: 29748501) »