Anti-Glucose 6 Phosphate Dehydrogenase antibody (ab87230)
Key features and details
- Rabbit polyclonal to Glucose 6 Phosphate Dehydrogenase
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
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- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-Glucose 6 Phosphate Dehydrogenase antibody
See all Glucose 6 Phosphate Dehydrogenase primary antibodies -
Description
Rabbit polyclonal to Glucose 6 Phosphate Dehydrogenase -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Sheep, Horse, Cow, Cat, Dog, Turkey, Zebrafish, Macaque monkey, Chinese hamster -
Immunogen
Synthetic peptide corresponding to Human Glucose 6 Phosphate Dehydrogenase aa 450 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
(Peptide available asab87368) -
Positive control
- Recombinant human Glucose 6 Phosphate Dehydrogenase protein (ab126671) can be used as a positive control in WB. This antibody gave a positive signal in RAW 264.7 and Jurkat Whole Cell Lysates, and in Human Lymph node Tissue Lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Assay kits
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab87230 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (1) |
Use a concentration of 1 µg/ml. Detects a band of approximately 59 kDa (predicted molecular weight: 59 kDa).
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IHC-P |
Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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ICC/IF |
Use a concentration of 1 µg/ml.
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Notes |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 59 kDa (predicted molecular weight: 59 kDa). |
IHC-P
Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
ICC/IF
Use a concentration of 1 µg/ml. |
Target
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Function
Catalyzes the rate-limiting step of the oxidative pentose-phosphate pathway, which represents a route for the dissimilation of carbohydrates besides glycolysis. The main function of this enzyme is to provide reducing power (NADPH) and pentose phosphates for fatty acid and nucleic acid synthesis. -
Tissue specificity
Isoform Long is found in lymphoblasts, granulocytes and sperm. -
Pathway
Carbohydrate degradation; pentose phosphate pathway; D-ribulose 5-phosphate from D-glucose 6-phosphate (oxidative stage): step 1/3. -
Involvement in disease
Anemia, non-spherocytic hemolytic, due to G6PD deficiency -
Sequence similarities
Belongs to the glucose-6-phosphate dehydrogenase family. -
Post-translational
modificationsAcetylated by ELP3 at Lys-403; acetylation inhibits its homodimerization and enzyme activity. Deacetylated by SIRT2 at Lys-403; deacetylation stimulates its enzyme activity. - Information by UniProt
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Database links
- Entrez Gene: 281179 Cow
- Entrez Gene: 81088 Dog
- Entrez Gene: 2539 Human
- Entrez Gene: 14381 Mouse
- Entrez Gene: 24377 Rat
- Entrez Gene: 443211 Sheep
- Omim: 305900 Human
- SwissProt: P11413 Human
see all -
Alternative names
- G6PD antibody
- G6PD_HUMAN antibody
- G6PD1 antibody
see all
Images
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All lanes : Anti-Glucose 6 Phosphate Dehydrogenase antibody (ab87230) at 1 µg/ml
Lane 1 : RAW 264.7 (Mouse leukaemic monocyte macrophage cell line) Whole Cell Lysate
Lane 2 : Human lymph node tissue lysate - total protein (ab29871)
Lane 3 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 59 kDa
Observed band size: 59 kDa
Additional bands at: 95 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 30 seconds -
ab87230 stained in Hela cells. Cells were fixed with 4% paraformaldehyde (10min) at room temperature and incubated with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% triton for 1h at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab87230 at 1µg/ml and ab7291 (Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control) at 1/1000 dilution overnight at +4°C. The secondary antibodies were ab150120 (pseudo-colored red) and ab150081 (colored green) used at 1 ug/ml for 1hour at room temperature. DAPI was used to stain the cell nuclei (colored blue) at a concentration of 1.43µM for 1hour at room temperature
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IHC image of Glucose 6 Phosphate Dehydrogenase staining in human breast carcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab87230, 10µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (4)
ab87230 has been referenced in 4 publications.
- Mattu S et al. Nrf2 Mutation/Activation Is Dispensable for the Development of Chemically Induced Mouse HCC. Cell Mol Gastroenterol Hepatol 13:113-127 (2022). PubMed: 34530178
- Hwang S et al. Correcting glucose-6-phosphate dehydrogenase deficiency with a small-molecule activator. Nat Commun 9:4045 (2018). PubMed: 30279493
- Kim S et al. Arsenite-induced changes in hepatic protein abundance in cynomolgus monkeys (Macaca fascicularis). Proteomics 14:1833-43 (2014). PubMed: 24866292
- Patrinostro X et al. A model of glucose-6-phosphate dehydrogenase deficiency in the zebrafish. Exp Hematol 41:697-710.e2 (2013). PubMed: 23603363