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    glucose-6-phosphate-dehydrogenase-antibody-ab993.pdf

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Signal Transduction Metabolism Energy Metabolism
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Anti-Glucose 6 Phosphate Dehydrogenase antibody (ab993)

  • Datasheet
  • SDS
Reviews (12)Q&A (3)References (31)

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Western blot - Anti-Glucose 6 Phosphate Dehydrogenase antibody (ab993)
  • Immunocytochemistry/ Immunofluorescence - Anti-Glucose 6 Phosphate Dehydrogenase antibody (ab993)
  • Immunoprecipitation - Anti-Glucose 6 Phosphate Dehydrogenase antibody (ab993)

Key features and details

  • Rabbit polyclonal to Glucose 6 Phosphate Dehydrogenase
  • Suitable for: IP, WB, ICC/IF
  • Reacts with: Mouse, Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-Glucose 6 Phosphate Dehydrogenase antibody
    See all Glucose 6 Phosphate Dehydrogenase primary antibodies
  • Description

    Rabbit polyclonal to Glucose 6 Phosphate Dehydrogenase
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IP
    Mouse
    WB
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide within Human Glucose 6 Phosphate Dehydrogenase aa 50-100. The exact sequence is proprietary.
    Database link: P11413

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7
    Preservative: 0.1% Sodium azide
    Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Metabolism
    • Energy Metabolism
    • Cardiovascular
    • Atherosclerosis
    • Diabetes associated
    • Cardiovascular
    • Heart
    • Cardiac metabolism
    • Cancer
    • Cancer Metabolism
    • Metabolic signaling pathway
    • Metabolism of carbohydrates
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Carbohydrate metabolism
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Energy transfer pathways
    • Energy Metabolism
    • Metabolism
    • Types of disease
    • Diabetes
    • Metabolism
    • Types of disease
    • Cancer
    • Metabolism
    • Types of disease
    • Heart disease

Associated products

  • Assay kits

    • Glucose 6 Phosphate Dehydrogenase Assay Kit (Colorimetric) (ab102529)
    • Glucose 6 Phosphate Dehydrogenase Activity Assay Kit (Fluorometric) (ab176722)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • HeLa whole cell lysate (ab150035)
    • HeLa whole cell lysate (ab29545)
    • NIH/3T3 whole cell lysate (ab7179)
  • Recombinant Protein

    • Recombinant human Glucose 6 Phosphate Dehydrogenase protein (ab126671)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab993 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
ICC/IF
Human
IP
Mouse
WB
Human
All applications
Chimpanzee
Baboon
Cynomolgus monkey
Rhesus monkey
Gorilla
Application Abreviews Notes
IP
Use at 2-10 µg/mg of lysate.
WB (6)
1/1000 - 1/10000. Predicted molecular weight: 59 kDa.
ICC/IF (4)
Use a concentration of 5 µg/ml.
Notes
IP
Use at 2-10 µg/mg of lysate.
WB
1/1000 - 1/10000. Predicted molecular weight: 59 kDa.
ICC/IF
Use a concentration of 5 µg/ml.

Target

  • Function

    Catalyzes the rate-limiting step of the oxidative pentose-phosphate pathway, which represents a route for the dissimilation of carbohydrates besides glycolysis. The main function of this enzyme is to provide reducing power (NADPH) and pentose phosphates for fatty acid and nucleic acid synthesis.
  • Tissue specificity

    Isoform Long is found in lymphoblasts, granulocytes and sperm.
  • Pathway

    Carbohydrate degradation; pentose phosphate pathway; D-ribulose 5-phosphate from D-glucose 6-phosphate (oxidative stage): step 1/3.
  • Involvement in disease

    Anemia, non-spherocytic hemolytic, due to G6PD deficiency
  • Sequence similarities

    Belongs to the glucose-6-phosphate dehydrogenase family.
  • Post-translational
    modifications

    Acetylated by ELP3 at Lys-403; acetylation inhibits its homodimerization and enzyme activity. Deacetylated by SIRT2 at Lys-403; deacetylation stimulates its enzyme activity.
  • Target information above from: UniProt accession P11413 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 2539 Human
    • Entrez Gene: 14381 Mouse
    • Omim: 305900 Human
    • SwissProt: P11413 Human
    • SwissProt: Q00612 Mouse
    • Unigene: 461047 Human
    • Unigene: 684904 Human
    • Unigene: 27210 Mouse
    • Alternative names

      • G6PD antibody
      • G6PD_HUMAN antibody
      • G6PD1 antibody
      • G6pdx antibody
      • Glucose 6 phosphate 1 dehydrogenase antibody
      • Glucose 6 phosphate dehydrogenase antibody
      • Glucose 6 phosphate dehydrogenase, G6PD antibody
      • Glucose-6-phosphate 1-dehydrogenase antibody
      • MET19 antibody
      • POS10 antibody
      • Zwf1p antibody
      see all

    Images

    • Western blot - Anti-Glucose 6 Phosphate Dehydrogenase antibody (ab993)
      Western blot - Anti-Glucose 6 Phosphate Dehydrogenase antibody (ab993)
      All lanes : Anti-Glucose 6 Phosphate Dehydrogenase antibody (ab993) at 1 µg/ml

      Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
      Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
      Lane 3 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate

      Lysates/proteins at 50 µg per lane.

      Developed using the ECL technique.

      Predicted band size: 59 kDa


      Exposure time: 30 seconds
    • Immunocytochemistry/ Immunofluorescence - Anti-Glucose 6 Phosphate Dehydrogenase antibody (ab993)
      Immunocytochemistry/ Immunofluorescence - Anti-Glucose 6 Phosphate Dehydrogenase antibody (ab993)
      ICC/IF image of ab993 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab993 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    • Immunoprecipitation - Anti-Glucose 6 Phosphate Dehydrogenase antibody (ab993)
      Immunoprecipitation - Anti-Glucose 6 Phosphate Dehydrogenase antibody (ab993)

      Glucose 6 Phosphate Dehydrogenase was immunoprecipitated from NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab993 at 6 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab993 at 1 µg/ml.

      Lane 1: ab993 (batch 3) IP in NIH/3T3 whole cell lysate.

      Lane 2: ab993 (batch 4) IP in NIH/3T3 whole cell lysate.

      Lane 3: Control IgG IP in NIH/3T3 whole cell lysate.

      Detection: Chemiluminescence with exposure time of 30 seconds.

    Protocols

    • Immunoprecipitation protocols
    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
    • SDS
  • References (31)

    Publishing research using ab993? Please let us know so that we can cite the reference in this datasheet.

    ab993 has been referenced in 31 publications.

    • Wen J  et al. Effects of glucose metabolism pathways on nuclear and cytoplasmic maturation of pig oocytes. Sci Rep 10:2782 (2020). PubMed: 32066834
    • Liu J  et al. Ginsenoside Rh2 pretreatment and withdrawal reactivated the pentose phosphate pathway to ameliorate intracellular redox disturbance and promoted intratumoral penetration of adriamycin. Redox Biol 32:101452 (2020). PubMed: 32067911
    • Li Z  et al. APC-Cdh1 Regulates Neuronal Apoptosis Through Modulating Glycolysis and Pentose-Phosphate Pathway After Oxygen-Glucose Deprivation and Reperfusion. Cell Mol Neurobiol 39:123-135 (2019). PubMed: 30460429
    • Tao L  et al. Rev-erba inhibits proliferation by reducing glycolytic flux and pentose phosphate pathway in human gastric cancer cells. Oncogenesis 8:57 (2019). PubMed: 31591390
    • Min KH & Lee W Alteration of mitochondrial DNA content modulates antioxidant enzyme expressions and oxidative stress in myoblasts. Korean J Physiol Pharmacol 23:519-528 (2019). PubMed: 31680774
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-10 of 15 Abreviews or Q&A

    Western blot abreview for Anti-Glucose 6 Phosphate Dehydrogenase antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (HCT116 colon cancer cells)
    Gel Running Conditions
    Reduced Denaturing (10)
    Loading amount
    20 µg
    Specification
    HCT116 colon cancer cells
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Dr. Míriam Tarrado-Castellarnau

    Verified customer

    Submitted Oct 02 2018

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Glucose 6 Phosphate Dehydrogenase antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (HEK293)
    Permeabilization
    Yes - 0.1% Triton X100
    Specification
    HEK293
    Blocking step
    Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Sep 30 2017

    Western blot abreview for Anti-Glucose 6 Phosphate Dehydrogenase antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Loading amount
    30 µg
    Gel Running Conditions
    Reduced Denaturing
    Sample
    Mouse Tissue lysate - whole (Mouse brain)
    Specification
    Mouse brain
    Blocking step
    Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    Submitted Jun 02 2014

    Western blot abreview for Anti-Glucose 6 Phosphate Dehydrogenase antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Loading amount
    20 µg
    Gel Running Conditions
    Reduced Denaturing (12)
    Sample
    Mouse Tissue lysate - other (Lung cytosol)
    Specification
    Lung cytosol
    Blocking step
    (agent) for 1 hour(s) and 30 minute(s) · Concentration: 5% · Temperature: RT°C
    Read More

    Dr. Amit Agarwal

    Verified customer

    Submitted Jul 19 2013

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Glucose 6 Phosphate Dehydrogenase antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (WBCs)
    Specification
    WBCs
    Fixative
    Formaldehyde
    Permeabilization
    Yes - 0.1% Triton X-100
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    Submitted Apr 11 2012

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Glucose 6 Phosphate Dehydrogenase antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Mouse Cell (Leukocytes from bone marrow)
    Specification
    Leukocytes from bone marrow
    Fixative
    Methanol
    Permeabilization
    No
    Blocking step
    Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    Submitted Jan 28 2011

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Glucose 6 Phosphate Dehydrogenase antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Rat Cell (Bone marrow cells)
    Specification
    Bone marrow cells
    Fixative
    Methanol
    Permeabilization
    No
    Blocking step
    BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    Submitted Dec 09 2010

    Immunohistochemistry (Frozen sections) abreview for Anti-Glucose 6 Phosphate Dehydrogenase antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Rat Tissue sections (Rat testis)
    Specification
    Rat testis
    Fixative
    Formaldehyde
    Permeabilization
    Yes - 0.1% Triton X-100
    Blocking step
    BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    Submitted Nov 01 2010

    Western blot abreview for Anti-Glucose 6 Phosphate Dehydrogenase antibody

    Poor
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Schizosaccharomyces pombe Cell lysate - other (whole cell lysate)
    Loading amount
    100 µg
    Specification
    whole cell lysate
    Gel Running Conditions
    Reduced Denaturing (12)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
    Read More

    Abcam user community

    Verified customer

    Submitted Aug 02 2010

    Immunohistochemistry (Frozen sections) abreview for Anti-Glucose 6 Phosphate Dehydrogenase antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (Skeletal muscle)
    Specification
    Skeletal muscle
    Fixative
    Formaldehyde
    Permeabilization
    Yes - 0.1% Triton X-100
    Blocking step
    Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    Submitted Mar 19 2010

    1-10 of 15 Abreviews or Q&A

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