Anti-Glucose 6 Phosphate Dehydrogenase antibody (ab993)
Key features and details
- Rabbit polyclonal to Glucose 6 Phosphate Dehydrogenase
- Suitable for: IP, WB, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-Glucose 6 Phosphate Dehydrogenase antibody
See all Glucose 6 Phosphate Dehydrogenase primary antibodies -
Description
Rabbit polyclonal to Glucose 6 Phosphate Dehydrogenase -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanIP MouseWB Human
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Immunogen
Synthetic peptide within Human Glucose 6 Phosphate Dehydrogenase aa 50-100. The exact sequence is proprietary.
Database link: P11413
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7
Preservative: 0.1% Sodium azide
Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Assay kits
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab993 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| ICC/IF |
Human
|
| IP |
Mouse
|
| WB |
Human
|
| All applications |
Chimpanzee
Baboon
Cynomolgus monkey
Rhesus monkey
Gorilla
|
| Application | Abreviews | Notes |
|---|---|---|
| IP |
Use at 2-10 µg/mg of lysate.
|
|
| WB | (6) |
1/1000 - 1/10000. Predicted molecular weight: 59 kDa.
|
| ICC/IF | (4) |
Use a concentration of 5 µg/ml.
|
| Notes |
|---|
|
IP
Use at 2-10 µg/mg of lysate. |
|
WB
1/1000 - 1/10000. Predicted molecular weight: 59 kDa. |
|
ICC/IF
Use a concentration of 5 µg/ml. |
Target
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Function
Catalyzes the rate-limiting step of the oxidative pentose-phosphate pathway, which represents a route for the dissimilation of carbohydrates besides glycolysis. The main function of this enzyme is to provide reducing power (NADPH) and pentose phosphates for fatty acid and nucleic acid synthesis. -
Tissue specificity
Isoform Long is found in lymphoblasts, granulocytes and sperm. -
Pathway
Carbohydrate degradation; pentose phosphate pathway; D-ribulose 5-phosphate from D-glucose 6-phosphate (oxidative stage): step 1/3. -
Involvement in disease
Anemia, non-spherocytic hemolytic, due to G6PD deficiency -
Sequence similarities
Belongs to the glucose-6-phosphate dehydrogenase family. -
Post-translational
modificationsAcetylated by ELP3 at Lys-403; acetylation inhibits its homodimerization and enzyme activity. Deacetylated by SIRT2 at Lys-403; deacetylation stimulates its enzyme activity. - Information by UniProt
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Database links
- Entrez Gene: 2539 Human
- Entrez Gene: 14381 Mouse
- Omim: 305900 Human
- SwissProt: P11413 Human
- SwissProt: Q00612 Mouse
- Unigene: 461047 Human
- Unigene: 684904 Human
- Unigene: 27210 Mouse
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Alternative names
- G6PD antibody
- G6PD_HUMAN antibody
- G6PD1 antibody
see all
Images
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Western blot - Anti-Glucose 6 Phosphate Dehydrogenase antibody (ab993)All lanes : Anti-Glucose 6 Phosphate Dehydrogenase antibody (ab993) at 1 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 59 kDa
Exposure time: 30 seconds -
Immunocytochemistry/ Immunofluorescence - Anti-Glucose 6 Phosphate Dehydrogenase antibody (ab993)ICC/IF image of ab993 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab993 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. -
Immunoprecipitation - Anti-Glucose 6 Phosphate Dehydrogenase antibody (ab993)Glucose 6 Phosphate Dehydrogenase was immunoprecipitated from NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab993 at 6 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab993 at 1 µg/ml.
Lane 1: ab993 (batch 3) IP in NIH/3T3 whole cell lysate.
Lane 2: ab993 (batch 4) IP in NIH/3T3 whole cell lysate.
Lane 3: Control IgG IP in NIH/3T3 whole cell lysate.
Detection: Chemiluminescence with exposure time of 30 seconds.
Protocols
References (31)
ab993 has been referenced in 31 publications.
- Wen J et al. Effects of glucose metabolism pathways on nuclear and cytoplasmic maturation of pig oocytes. Sci Rep 10:2782 (2020). PubMed: 32066834
- Liu J et al. Ginsenoside Rh2 pretreatment and withdrawal reactivated the pentose phosphate pathway to ameliorate intracellular redox disturbance and promoted intratumoral penetration of adriamycin. Redox Biol 32:101452 (2020). PubMed: 32067911
- Li Z et al. APC-Cdh1 Regulates Neuronal Apoptosis Through Modulating Glycolysis and Pentose-Phosphate Pathway After Oxygen-Glucose Deprivation and Reperfusion. Cell Mol Neurobiol 39:123-135 (2019). PubMed: 30460429
- Tao L et al. Rev-erba inhibits proliferation by reducing glycolytic flux and pentose phosphate pathway in human gastric cancer cells. Oncogenesis 8:57 (2019). PubMed: 31591390
- Min KH & Lee W Alteration of mitochondrial DNA content modulates antioxidant enzyme expressions and oxidative stress in myoblasts. Korean J Physiol Pharmacol 23:519-528 (2019). PubMed: 31680774
