Anti-Glucose 6 Phosphate Dehydrogenase antibody (ab993)
- Datasheet
- References (20)
- Protocols
Overview
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Product nameAnti-Glucose 6 Phosphate Dehydrogenase antibody
See all Glucose 6 Phosphate Dehydrogenase primary antibodies -
DescriptionRabbit polyclonal to Glucose 6 Phosphate Dehydrogenase
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Host speciesRabbit
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Tested applicationsSuitable for: IHC-Fr, IP, WB, ICC/IFmore details
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Species reactivityReacts with: Mouse, Rat, Human
Predicted to work with: Chimpanzee, Baboon, Cynomolgus monkey, Rhesus monkey, Gorilla
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Immunogen
Synthetic peptide within Human Glucose 6 Phosphate Dehydrogenase aa 50-100. The exact sequence is proprietary.
Database link: P11413 -
Positive control
- WB: HeLa, HEK-293T and NIH/3T3 whole cell lysates;. ICC/IF: HUVEC cells. IHC-Fr: Mouse skeletal muscle tissue.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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Storage bufferPreservative: 0.1% Sodium azide
Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris -
Concentration information loading... -
PurityImmunogen affinity purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Assay kits
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab993 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-Fr | 1/500. | |
| IP | Use at 2-10 µg/mg of lysate. | |
| WB | 1/1000 - 1/10000. Predicted molecular weight: 59 kDa. | |
| ICC/IF | Use a concentration of 5 µg/ml. |
Target
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FunctionCatalyzes the rate-limiting step of the oxidative pentose-phosphate pathway, which represents a route for the dissimilation of carbohydrates besides glycolysis. The main function of this enzyme is to provide reducing power (NADPH) and pentose phosphates for fatty acid and nucleic acid synthesis.
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Tissue specificityIsoform Long is found in lymphoblasts, granulocytes and sperm.
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PathwayCarbohydrate degradation; pentose phosphate pathway; D-ribulose 5-phosphate from D-glucose 6-phosphate (oxidative stage): step 1/3.
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Involvement in diseaseAnemia, non-spherocytic hemolytic, due to G6PD deficiency
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Sequence similaritiesBelongs to the glucose-6-phosphate dehydrogenase family.
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Post-translational
modificationsAcetylated by ELP3 at Lys-403; acetylation inhibits its homodimerization and enzyme activity. Deacetylated by SIRT2 at Lys-403; deacetylation stimulates its enzyme activity. - Information by UniProt
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Database links
- Entrez Gene: 2539 Human
- Entrez Gene: 14381 Mouse
- Entrez Gene: 24377 Rat
- Omim: 305900 Human
- SwissProt: P11413 Human
- SwissProt: Q00612 Mouse
- SwissProt: P05370 Rat
- Unigene: 461047 Human
see all -
Alternative names
- G6PD antibody
- G6PD_HUMAN antibody
- G6PD1 antibody
see all
Images
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Western blot - Anti-Glucose 6 Phosphate Dehydrogenase antibody (ab993)All lanes : Anti-Glucose 6 Phosphate Dehydrogenase antibody (ab993) at 1 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 59 kDa
Exposure time: 30 seconds -
Immunocytochemistry/ Immunofluorescence - Anti-Glucose 6 Phosphate Dehydrogenase antibody (ab993)ICC/IF image of ab993 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab993 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. -
Immunoprecipitation - Anti-Glucose 6 Phosphate Dehydrogenase antibody (ab993)Glucose 6 Phosphate Dehydrogenase was immunoprecipitated from NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab993 at 6 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab993 at 1 µg/ml.
Lane 1: ab993 (batch 3) IP in NIH/3T3 whole cell lysate.
Lane 2: ab993 (batch 4) IP in NIH/3T3 whole cell lysate.
Lane 3: Control IgG IP in NIH/3T3 whole cell lysate.
Detection: Chemiluminescence with exposure time of 30 seconds.
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Immunohistochemistry (Frozen sections) - Anti-Glucose 6 Phosphate Dehydrogenase antibody (ab993)This image is courtesy of an anonymous Abreviewab993 staining Glucose 6 phosphate dehydrogenase in Mouse skeletal muscle tissue sections by IHC-Fr (frozen sections). Tissue was fixed with formaldehyde, permeabilized with 0.1% Triton X-100 and blocked with 5% serum for 2 hours at 25°C. Samples were incubated with primary antibody (1/500 in PBS Tween 20) for 12 hours at 4°C. An Alexa Fluor®-conjugated goat anti-rabbit IgG polyclonal (1/500) was used as secondary antibody.
Protocols
References
This product has been referenced in:
- Putker M et al. Mammalian Circadian Period, But Not Phase and Amplitude, Is Robust Against Redox and Metabolic Perturbations. Antioxid Redox Signal 28:507-520 (2018). Read more (PubMed: 28506121) »
- Chhabra A et al. Glucose-6-phosphate dehydrogenase is critical for suppression of cardiac hypertrophy by H2S. Cell Death Discov 4:6 (2018). Read more (PubMed: 29531803) »
