For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome
Synthetic peptide within Human Glucose 6 Phosphate Dehydrogenase aa 50-100. The exact sequence is proprietary.
Database link: P11413
Our Abpromise guarantee covers the use of ab993 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at 2-10 µg/mg of lysate.|
|WB||1/1000 - 1/10000. Predicted molecular weight: 59 kDa.|
|ICC/IF||Use a concentration of 5 µg/ml.|
Glucose 6 Phosphate Dehydrogenase was immunoprecipitated from NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab993 at 6 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab993 at 1 µg/ml.
Lane 1: ab993 (batch 3) IP in NIH/3T3 whole cell lysate.
Lane 2: ab993 (batch 4) IP in NIH/3T3 whole cell lysate.
Lane 3: Control IgG IP in NIH/3T3 whole cell lysate.
Detection: Chemiluminescence with exposure time of 30 seconds.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"