Key features and details
- Mouse monoclonal [AT2F6] to Glucose 6 Phosphate Dehydrogenase
- Suitable for: ICC/IF, WB, ELISA
- Reacts with: Human
- Isotype: IgG2b
Product nameAnti-Glucose 6 Phosphate Dehydrogenase antibody [AT2F6]
See all Glucose 6 Phosphate Dehydrogenase primary antibodies
DescriptionMouse monoclonal [AT2F6] to Glucose 6 Phosphate Dehydrogenase
Tested applicationsSuitable for: ICC/IF, WB, ELISAmore details
Species reactivityReacts with: Human
Recombinant fragment corresponding to Human Glucose 6 Phosphate Dehydrogenase aa 35-506 (internal sequence).
Database link: P11413
- MCF7 cell lysate ICC/IF: HeLa cells
This product was changed from ascites to tissue culture supernatant on 11th April 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 10% Glycerol
Concentration information loading...
PurityProtein A purified
Light chain typekappa
- Pathways and Processes
- Metabolic signaling pathways
- Energy transfer pathways
- Energy Metabolism
Our Abpromise guarantee covers the use of ab91034 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/2000. Predicted molecular weight: 59 kDa.|
|ELISA||Use at an assay dependent concentration.|
FunctionCatalyzes the rate-limiting step of the oxidative pentose-phosphate pathway, which represents a route for the dissimilation of carbohydrates besides glycolysis. The main function of this enzyme is to provide reducing power (NADPH) and pentose phosphates for fatty acid and nucleic acid synthesis.
Tissue specificityIsoform Long is found in lymphoblasts, granulocytes and sperm.
PathwayCarbohydrate degradation; pentose phosphate pathway; D-ribulose 5-phosphate from D-glucose 6-phosphate (oxidative stage): step 1/3.
Involvement in diseaseAnemia, non-spherocytic hemolytic, due to G6PD deficiency
Sequence similaritiesBelongs to the glucose-6-phosphate dehydrogenase family.
modificationsAcetylated by ELP3 at Lys-403; acetylation inhibits its homodimerization and enzyme activity. Deacetylated by SIRT2 at Lys-403; deacetylation stimulates its enzyme activity.
- Information by UniProt
- G6PD antibody
- G6PD_HUMAN antibody
- G6PD1 antibody
All lanes : Anti-Glucose 6 Phosphate Dehydrogenase antibody [AT2F6] (ab91034) at 1/1000 dilution
Lane 1 : MCF7 cell lysate
Lane 2 : HeLa cell lysate
All lanes : Goat Anti-Mouse HRP conjugated
Developed using the ECL technique.
Predicted band size: 59 kDa
Immunocytochemistry/ Immunofluorescence analysis of HeLa cells labeling Glucose 6 Phosphate Dehydrogenase with ab91034 at 1/100 dilution. The secondary antibody used was Alexa Fluor 488. DAPI was used to stain the cell nucleus (blue).
ab91034 has not yet been referenced specifically in any publications.