Anti-Glucose 6 Phosphate Dehydrogenase antibody [AT2F6] (ab91034)
Key features and details
- Mouse monoclonal [AT2F6] to Glucose 6 Phosphate Dehydrogenase
- Suitable for: ICC/IF, WB
- Reacts with: Human
- Isotype: IgG2b
Overview
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Product name
Anti-Glucose 6 Phosphate Dehydrogenase antibody [AT2F6]
See all Glucose 6 Phosphate Dehydrogenase primary antibodies -
Description
Mouse monoclonal [AT2F6] to Glucose 6 Phosphate Dehydrogenase -
Host species
Mouse -
Tested applications
Suitable for: ICC/IF, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to Human Glucose 6 Phosphate Dehydrogenase aa 35-506 (internal sequence).
Database link: P11413 -
Positive control
- MCF7 cell lysate ICC/IF: HeLa cells
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General notes
This product was changed from ascites to tissue culture supernatant on 11th April 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 10% Glycerol -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
AT2F6 -
Isotype
IgG2b -
Light chain type
kappa -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab91034 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
ICC/IF |
1/100.
|
|
WB | (2) |
1/1000 - 1/2000. Predicted molecular weight: 59 kDa.
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Notes |
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ICC/IF
1/100. |
WB
1/1000 - 1/2000. Predicted molecular weight: 59 kDa. |
Target
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Function
Catalyzes the rate-limiting step of the oxidative pentose-phosphate pathway, which represents a route for the dissimilation of carbohydrates besides glycolysis. The main function of this enzyme is to provide reducing power (NADPH) and pentose phosphates for fatty acid and nucleic acid synthesis. -
Tissue specificity
Isoform Long is found in lymphoblasts, granulocytes and sperm. -
Pathway
Carbohydrate degradation; pentose phosphate pathway; D-ribulose 5-phosphate from D-glucose 6-phosphate (oxidative stage): step 1/3. -
Involvement in disease
Anemia, non-spherocytic hemolytic, due to G6PD deficiency -
Sequence similarities
Belongs to the glucose-6-phosphate dehydrogenase family. -
Post-translational
modificationsAcetylated by ELP3 at Lys-403; acetylation inhibits its homodimerization and enzyme activity. Deacetylated by SIRT2 at Lys-403; deacetylation stimulates its enzyme activity. - Information by UniProt
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Database links
- Entrez Gene: 2539 Human
- Omim: 305900 Human
- SwissProt: P11413 Human
- Unigene: 461047 Human
- Unigene: 684904 Human
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Alternative names
- G6PD antibody
- G6PD_HUMAN antibody
- G6PD1 antibody
see all
Images
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All lanes : Anti-Glucose 6 Phosphate Dehydrogenase antibody [AT2F6] (ab91034) at 1/1000 dilution
Lane 1 : MCF7 cell lysate
Lane 2 : HeLa cell lysate
Secondary
All lanes : Goat Anti-Mouse HRP conjugated
Developed using the ECL technique.
Predicted band size: 59 kDa -
Immunocytochemistry/ Immunofluorescence analysis of HeLa cells labeling Glucose 6 Phosphate Dehydrogenase with ab91034 at 1/100 dilution. The secondary antibody used was Alexa Fluor 488. DAPI was used to stain the cell nucleus (blue).
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab91034 has not yet been referenced specifically in any publications.