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Rabbit monoclonal [EPR6291] to Glucose 6 Phosphate Dehydrogenase
Flow Cyt more details Unsuitable for:
ICC,IHC-P or IP
Synthetic peptide within Human Glucose 6 Phosphate Dehydrogenase aa 100-200. The exact sequence is proprietary.
293T, MOLT4, HUVEC and HeLa cell lysates; Permeablized HeLa cells.
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab ® patents
This product is a recombinant rabbit monoclonal antibody.
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Preservative: 0.05% Sodium azide Constituents: 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
Tissue culture supernatant
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/10000 - 1/50000. Detects a band of approximately 59 kDa (predicted molecular weight: 59 kDa).
1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
Is unsuitable for ICC,IHC-P or IP.
Catalyzes the rate-limiting step of the oxidative pentose-phosphate pathway, which represents a route for the dissimilation of carbohydrates besides glycolysis. The main function of this enzyme is to provide reducing power (NADPH) and pentose phosphates for fatty acid and nucleic acid synthesis.
Isoform Long is found in lymphoblasts, granulocytes and sperm.
Carbohydrate degradation; pentose phosphate pathway; D-ribulose 5-phosphate from D-glucose 6-phosphate (oxidative stage): step 1/3.
Involvement in disease
Anemia, non-spherocytic hemolytic, due to G6PD deficiency
Belongs to the glucose-6-phosphate dehydrogenase family.
Acetylated by ELP3 at Lys-403; acetylation inhibits its homodimerization and enzyme activity. Deacetylated by SIRT2 at Lys-403; deacetylation stimulates its enzyme activity.
Information by UniProt
Western blot - Anti-Glucose 6 Phosphate Dehydrogenase antibody [EPR6291] (ab124738)
All lanes : Anti-Glucose 6 Phosphate Dehydrogenase antibody [EPR6291] (ab124738) at 1/10000 dilution Lane 1 : 293T cell lysate Lane 2 : MOLT4 cell lysate Lane 3 : HUVEC cell lysate Lane 4 : HeLa cell lysate Lysates/proteins at 10 µg per lane. Secondary All lanes : Goat anti-Rabbit HRP at 1/2000 dilution Predicted band size: 59 kDa
Flow Cytometry - Anti-Glucose 6 Phosphate Dehydrogenase antibody [EPR6291] (ab124738)
ab124738 at 1/10 dilution staining Glucose 6 Phosphate Dehydrogenase in permeablized HeLa cells (red) or a rabbit IgG (negative) (green).
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"