Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6292] to Glucose 6 Phosphate Dehydrogenase
- Suitable for: WB, IHC-P, Flow Cyt
- Reacts with: Human
Product nameAnti-Glucose 6 Phosphate Dehydrogenase antibody [EPR6292]
See all Glucose 6 Phosphate Dehydrogenase primary antibodies
DescriptionRabbit monoclonal [EPR6292] to Glucose 6 Phosphate Dehydrogenase
Tested applicationsSuitable for: WB, IHC-P, Flow Cytmore details
Unsuitable for: IP
Species reactivityReacts with: Human
Synthetic peptide corresponding to residues in the Human Glucose 6 Phosphate Dehydrogenase protein (UniProt ID: P11413).
- WB: Molt 4, A549, MCF7, HeLa, and HepG2 cell lysates
IHC-P: Human testis tissue
- WB: Molt 4, A549, MCF7, HeLa, and HepG2 cell lysates
Cellular localisation: centrosome, cytosol, internal side of plasma membrane, intracellular membrane bounded organelle. There are 3 isoforms produced by alternative splicing.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
Dissociation constant (KD)KD = 4.30 x 10 -11 M Learn more about KD
Storage bufferpH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
- Pathways and Processes
- Metabolic signaling pathways
- Energy transfer pathways
- Energy Metabolism
Our Abpromise guarantee covers the use of ab133525 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Detects a band of approximately 58 kDa (predicted molecular weight: 59 kDa).|
|IHC-P||1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|Flow Cyt||1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
FunctionCatalyzes the rate-limiting step of the oxidative pentose-phosphate pathway, which represents a route for the dissimilation of carbohydrates besides glycolysis. The main function of this enzyme is to provide reducing power (NADPH) and pentose phosphates for fatty acid and nucleic acid synthesis.
Tissue specificityIsoform Long is found in lymphoblasts, granulocytes and sperm.
PathwayCarbohydrate degradation; pentose phosphate pathway; D-ribulose 5-phosphate from D-glucose 6-phosphate (oxidative stage): step 1/3.
Involvement in diseaseAnemia, non-spherocytic hemolytic, due to G6PD deficiency
Sequence similaritiesBelongs to the glucose-6-phosphate dehydrogenase family.
modificationsAcetylated by ELP3 at Lys-403; acetylation inhibits its homodimerization and enzyme activity. Deacetylated by SIRT2 at Lys-403; deacetylation stimulates its enzyme activity.
- Information by UniProt
- G6PD antibody
- G6PD_HUMAN antibody
- G6PD1 antibody
All lanes : Anti-Glucose 6 Phosphate Dehydrogenase antibody [EPR6292] (ab133525) at 1/1000 dilution
Lane 1 : Molt 4 cell lysate
Lane 2 : A549 cell lysate
Lane 3 : MCF7 cell lysate
Lane 4 : HeLa cell lysate
Lane 5 : HepG2 cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat anti-rabbit at 1/2000 dilution
Predicted band size: 59 kDa
Observed band size: 58 kDa why is the actual band size different from the predicted?
Immunohistochemistry analysis of Paraffin Embedded Human testis tissue labelling Glucose 6 Phosphate Dehydrogenase with ab133525 at 1/50.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Equilibrium disassociation constant (KD)
Learn more about KD
Click here to learn more about KD
ab133525 has been referenced in 6 publications.
- Zhang Q et al. G6PD facilitates clear cell renal cell carcinoma invasion by enhancing MMP2 expression through ROS-MAPK axis pathway. Int J Oncol 57:197-212 (2020). PubMed: 32319593
- Kavanagh Williamson M et al. Upregulation of Glucose Uptake and Hexokinase Activity of Primary Human CD4+ T Cells in Response to Infection with HIV-1. Viruses 10:N/A (2018). PubMed: 29518929
- Ju HQ et al. Disrupting G6PD-mediated Redox homeostasis enhances chemosensitivity in colorectal cancer. Oncogene 36:6282-6292 (2017). PubMed: 28692052
- Zhang Q et al. Overexpression of G6PD Represents a Potential Prognostic Factor in Clear Cell Renal Cell Carcinoma. J Cancer 8:665-673 (2017). PubMed: 28367246
- Zhang Q et al. G6PD promotes renal cell carcinoma proliferation through positive feedback regulation of p-STAT3. Oncotarget 8:109043-109060 (2017). PubMed: 29312589
- Deng C et al. Low nanomolar concentrations of Cucurbitacin-I induces G2/M phase arrest and apoptosis by perturbing redox homeostasis in gastric cancer cells in vitro and in vivo. Cell Death Dis 7:e2106 (2016). PubMed: 26890145