Recombinant
RabMAb

Recombinant Anti-Glucose 6 Phosphate Dehydrogenase antibody [EPR6292] (ab133525)

Overview

  • Product name

    Anti-Glucose 6 Phosphate Dehydrogenase antibody [EPR6292]
    See all Glucose 6 Phosphate Dehydrogenase primary antibodies
  • Description

    Rabbit monoclonal [EPR6292] to Glucose 6 Phosphate Dehydrogenase
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC, Flow Cytmore details
    Unsuitable for: IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to residues in the Human Glucose 6 Phosphate Dehydrogenase protein (UniProt ID: P11413).

  • Positive control

    • WB: Molt 4, A549, MCF7, HeLa, and HepG2 cell lysates
      IHC-P: Human testis tissue
  • General notes

    Cellular localisation: centrosome, cytosol, internal side of plasma membrane, intracellular membrane bounded organelle. There are 3 isoforms produced by alternative splicing.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab133525 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 58 kDa (predicted molecular weight: 59 kDa).
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC 1/50 - 1/100.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

  • Application notes
    Is unsuitable for IP.
  • Target

    • Function

      Catalyzes the rate-limiting step of the oxidative pentose-phosphate pathway, which represents a route for the dissimilation of carbohydrates besides glycolysis. The main function of this enzyme is to provide reducing power (NADPH) and pentose phosphates for fatty acid and nucleic acid synthesis.
    • Tissue specificity

      Isoform Long is found in lymphoblasts, granulocytes and sperm.
    • Pathway

      Carbohydrate degradation; pentose phosphate pathway; D-ribulose 5-phosphate from D-glucose 6-phosphate (oxidative stage): step 1/3.
    • Involvement in disease

      Anemia, non-spherocytic hemolytic, due to G6PD deficiency
    • Sequence similarities

      Belongs to the glucose-6-phosphate dehydrogenase family.
    • Post-translational
      modifications

      Acetylated by ELP3 at Lys-403; acetylation inhibits its homodimerization and enzyme activity. Deacetylated by SIRT2 at Lys-403; deacetylation stimulates its enzyme activity.
    • Information by UniProt
    • Database links

    • Alternative names

      • G6PD antibody
      • G6PD_HUMAN antibody
      • G6PD1 antibody
      • G6pdx antibody
      • Glucose 6 phosphate 1 dehydrogenase antibody
      • Glucose 6 phosphate dehydrogenase antibody
      • Glucose 6 phosphate dehydrogenase, G6PD antibody
      • Glucose-6-phosphate 1-dehydrogenase antibody
      • MET19 antibody
      • POS10 antibody
      • Zwf1p antibody
      see all

    Images

    • All lanes : Anti-Glucose 6 Phosphate Dehydrogenase antibody [EPR6292] (ab133525) at 1/1000 dilution

      Lane 1 : Molt 4 cell lysate
      Lane 2 : A549 cell lysate
      Lane 3 : MCF7 cell lysate
      Lane 4 : HeLa cell lysate
      Lane 5 : HepG2 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit at 1/2000 dilution

      Predicted band size: 59 kDa
      Observed band size: 58 kDa
      why is the actual band size different from the predicted?

    • Immunohistochemistry analysis of Paraffin Embedded Human testis tissue labelling Glucose 6 Phosphate Dehydrogenase with ab133525 at 1/50.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    This product has been referenced in:

    • Kavanagh Williamson M  et al. Upregulation of Glucose Uptake and Hexokinase Activity of Primary Human CD4+ T Cells in Response to Infection with HIV-1. Viruses 10:N/A (2018). Read more (PubMed: 29518929) »
    • Ju HQ  et al. Disrupting G6PD-mediated Redox homeostasis enhances chemosensitivity in colorectal cancer. Oncogene 36:6282-6292 (2017). Read more (PubMed: 28692052) »
    See all 5 Publications for this product

    Customer reviews and Q&As

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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