Product nameAnti-Glucose 6 Phosphate Dehydrogenase antibody [EPR6292] (Alexa Fluor® 647)
See all Glucose 6 Phosphate Dehydrogenase primary antibodies
DescriptionRabbit monoclonal [EPR6292] to Glucose 6 Phosphate Dehydrogenase (Alexa Fluor® 647)
ConjugationAlexa Fluor® 647. Ex: 652nm, Em: 668nm
Tested applicationsSuitable for: ICC/IFmore details
Species reactivityReacts with: Human
Synthetic peptide within Human Glucose 6 Phosphate Dehydrogenase. The exact sequence is proprietary.
Database link: P11413
- ICC/IF: MCF7 cells
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or firstname.lastname@example.org.
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. Store In the Dark.
Dissociation constant (KD)KD = 4.30 x 10 -11 M Learn more about KD
Storage bufferpH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 1% BSA, 30% Glycerol
Concentration information loading...
PurityProtein A purified
- Pathways and Processes
- Metabolic signaling pathways
- Energy transfer pathways
- Energy Metabolism
- HepG2 cytoplasmic extract lysate (ab14659)
- MCF7 whole cell lysate (ab29537)
- MCF7 membrane extract lysate (ab29539)
- HeLa whole cell lysate (ab29545)
- HeLa membrane extract lysate (ab29547)
- MCF7 whole cell lysate (ab3871)
- HepG2 whole cell lysate (ab7900)
- A549 whole cell lysate (ab7910)
- MOLT4 whole cell lysate (ab7912)
Our Abpromise guarantee covers the use of ab204473 in the following tested applications.
This product gave a positive signal in MCF7 cells fixed with 100% methanol (5 min)
FunctionCatalyzes the rate-limiting step of the oxidative pentose-phosphate pathway, which represents a route for the dissimilation of carbohydrates besides glycolysis. The main function of this enzyme is to provide reducing power (NADPH) and pentose phosphates for fatty acid and nucleic acid synthesis.
Tissue specificityIsoform Long is found in lymphoblasts, granulocytes and sperm.
PathwayCarbohydrate degradation; pentose phosphate pathway; D-ribulose 5-phosphate from D-glucose 6-phosphate (oxidative stage): step 1/3.
Involvement in diseaseAnemia, non-spherocytic hemolytic, due to G6PD deficiency
Sequence similaritiesBelongs to the glucose-6-phosphate dehydrogenase family.
modificationsAcetylated by ELP3 at Lys-403; acetylation inhibits its homodimerization and enzyme activity. Deacetylated by SIRT2 at Lys-403; deacetylation stimulates its enzyme activity.
- Information by UniProt
- G6PD antibody
- G6PD_HUMAN antibody
- G6PD1 antibody
ab204473 staining Glucose 6 Phosphate Dehydrogenase in MCF7 cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab204473 at a 1/100 dilution (shown in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at a 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
ab204473 has not yet been referenced specifically in any publications.