
Recombinant Anti-Glucose 6 Phosphate Dehydrogenase antibody [EPR6292] - BSA and Azide free (ab218120)
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Overview
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Product name
Anti-Glucose 6 Phosphate Dehydrogenase antibody [EPR6292] - BSA and Azide free
See all Glucose 6 Phosphate Dehydrogenase primary antibodies -
Description
Rabbit monoclonal [EPR6292] to Glucose 6 Phosphate Dehydrogenase - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: ICC, IHC-P, Flow Cyt, WBmore details
Unsuitable for: IP -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human Glucose 6 Phosphate Dehydrogenase aa 450-550. The exact sequence is proprietary.
Database link: P11413 -
Positive control
- WB: Molt 4, A549, MCF7, HeLa, and HepG2 cell lysates
IHC-P: Human testis tissue
- WB: Molt 4, A549, MCF7, HeLa, and HepG2 cell lysates
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General notes
ab218120 is the carrier-free version of ab133525 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Ab218120 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
Cellular localisation: centrosome, cytosol, internal side of plasma membrane, intracellular membrane bounded organelle. There are 3 isoforms produced by alternative splicing. Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Dissociation constant (KD)
KD = 4.30 x 10 -11 M Learn more about KD -
Storage buffer
Constituent: PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR6292 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Assay kits
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Conjugation kits
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Positive Controls
- HepG2 cytoplasmic extract lysate (ab14659)
- MCF7 whole cell lysate (ab29537)
- MCF7 membrane extract lysate (ab29539)
- HeLa whole cell lysate (ab29545)
- HeLa membrane extract lysate (ab29547)
- MCF7 whole cell lysate (ab3871)
- HepG2 whole cell lysate (ab7900)
- A549 whole cell lysate (ab7910)
- MOLT4 whole cell lysate (ab7912)
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab218120 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC | Use at an assay dependent concentration. | |
IHC-P | Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
Flow Cyt | Use at an assay dependent concentration. | |
WB | Use at an assay dependent concentration. Detects a band of approximately 58 kDa (predicted molecular weight: 59 kDa). |
Target
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Function
Catalyzes the rate-limiting step of the oxidative pentose-phosphate pathway, which represents a route for the dissimilation of carbohydrates besides glycolysis. The main function of this enzyme is to provide reducing power (NADPH) and pentose phosphates for fatty acid and nucleic acid synthesis. -
Tissue specificity
Isoform Long is found in lymphoblasts, granulocytes and sperm. -
Pathway
Carbohydrate degradation; pentose phosphate pathway; D-ribulose 5-phosphate from D-glucose 6-phosphate (oxidative stage): step 1/3. -
Involvement in disease
Anemia, non-spherocytic hemolytic, due to G6PD deficiency -
Sequence similarities
Belongs to the glucose-6-phosphate dehydrogenase family. -
Post-translational
modificationsAcetylated by ELP3 at Lys-403; acetylation inhibits its homodimerization and enzyme activity. Deacetylated by SIRT2 at Lys-403; deacetylation stimulates its enzyme activity. - Information by UniProt
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Database links
- Entrez Gene: 2539 Human
- Omim: 305900 Human
- SwissProt: P11413 Human
- Unigene: 461047 Human
- Unigene: 684904 Human
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Alternative names
- G6PD antibody
- G6PD_HUMAN antibody
- G6PD1 antibody
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Datasheets and documents
References
ab218120 has not yet been referenced specifically in any publications.