Recombinant
RabMAb

Recombinant Anti-Glucose 6 Phosphate Dehydrogenase antibody [EPR6292] - BSA and Azide free (ab218120)

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Overview

  • Product name

    Anti-Glucose 6 Phosphate Dehydrogenase antibody [EPR6292] - BSA and Azide free
    See all Glucose 6 Phosphate Dehydrogenase primary antibodies

  • Description

    Rabbit monoclonal [EPR6292] to Glucose 6 Phosphate Dehydrogenase - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC, IHC-P, Flow Cyt, WBmore details
    Unsuitable for: IP

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide within Human Glucose 6 Phosphate Dehydrogenase aa 450-550. The exact sequence is proprietary.
    Database link: P11413

  • Positive control

    • WB: Molt 4, A549, MCF7, HeLa, and HepG2 cell lysates
      IHC-P: Human testis tissue

  • General notes

    ab218120 is the carrier-free version of ab133525 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    Ab218120 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Cellular localisation: centrosome, cytosol, internal side of plasma membrane, intracellular membrane bounded organelle. There are 3 isoforms produced by alternative splicing. Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab218120 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cyt Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Detects a band of approximately 58 kDa (predicted molecular weight: 59 kDa).
  • Application notes
    Is unsuitable for IP.

    • Target

    • Function

      Catalyzes the rate-limiting step of the oxidative pentose-phosphate pathway, which represents a route for the dissimilation of carbohydrates besides glycolysis. The main function of this enzyme is to provide reducing power (NADPH) and pentose phosphates for fatty acid and nucleic acid synthesis.

    • Tissue specificity

      Isoform Long is found in lymphoblasts, granulocytes and sperm.

    • Pathway

      Carbohydrate degradation; pentose phosphate pathway; D-ribulose 5-phosphate from D-glucose 6-phosphate (oxidative stage): step 1/3.

    • Involvement in disease

      Anemia, non-spherocytic hemolytic, due to G6PD deficiency

    • Sequence similarities

      Belongs to the glucose-6-phosphate dehydrogenase family.

    • Post-translational
      modifications

      Acetylated by ELP3 at Lys-403; acetylation inhibits its homodimerization and enzyme activity. Deacetylated by SIRT2 at Lys-403; deacetylation stimulates its enzyme activity.
    • Information by UniProt

    • Database links

      • Alternative names

        • G6PD antibody
        • G6PD_HUMAN antibody
        • G6PD1 antibody
        • G6pdx antibody
        • Glucose 6 phosphate 1 dehydrogenase antibody
        • Glucose 6 phosphate dehydrogenase antibody
        • Glucose 6 phosphate dehydrogenase, G6PD antibody
        • Glucose-6-phosphate 1-dehydrogenase antibody
        • MET19 antibody
        • POS10 antibody
        • Zwf1p antibody
        see all

      References

      ab218120 has not yet been referenced specifically in any publications.

      Publishing research using ab218120? Please let us know so that we can cite the reference in this datasheet.

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