Overview

  • Product name
    Anti-Glucose 6 phosphate isomerase antibody
    See all Glucose 6 phosphate isomerase primary antibodies
  • Description
    Goat polyclonal to Glucose 6 phosphate isomerase
  • Host species
    Goat
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Pig, Cynomolgus monkey, Orangutan
  • Immunogen

    Synthetic peptide:

    RERMFNGEKINYT

    by a Cysteine residue linker, corresponding to internal sequence amino acids 81-93 of Human Glucose 6 phosphate isomerase (NP_000166.2)

  • Positive control
    • Human heart lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab99189 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 63 kDa).

Target

  • Function
    Besides it's role as a glycolytic enzyme, mammalian GPI can function as a tumor-secreted cytokine and an angiogenic factor (AMF) that stimulates endothelial cell motility. GPI is also a neurotrophic factor (Neuroleukin) for spinal and sensory neurons.
  • Pathway
    Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 2/4.
  • Involvement in disease
    Defects in GPI are the cause of hemolytic anemia non-spherocytic due to glucose phosphate isomerase deficiency (HA-GPID) [MIM:613470]. It is a form of anemia in which there is no abnormal hemoglobin or spherocytosis. It is caused by glucose phosphate isomerase deficiency. Severe GPI deficiency can be associated with hydrops fetalis, immediate neonatal death and neurological impairment.
  • Sequence similarities
    Belongs to the GPI family.
  • Post-translational
    modifications
    Phosphorylation at Ser-185 by CK2 has been shown to decrease enzymatic activity and may contribute to secretion by a non-classical secretory pathway.
    ISGylated.
  • Cellular localization
    Cytoplasm. Secreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • AMF antibody
    • Aurocrine motility factor antibody
    • Autocrine motility factor antibody
    • DKFZp686C13233 antibody
    • EC 5.3.1.9 antibody
    • G6PI_HUMAN antibody
    • Glucose phosphate isomerase antibody
    • Glucose-6-phosphate isomerase antibody
    • GNPI antibody
    • GPI antibody
    • Gpi1 antibody
    • Hexose monophosphate isomerase antibody
    • Hexosephosphate isomerase antibody
    • Neuroleukin antibody
    • NLK antibody
    • Oxoisomerase antibody
    • PGI antibody
    • PHI antibody
    • Phosphoglucose isomerase antibody
    • Phosphohexomutase antibody
    • Phosphohexose isomerase antibody
    • Phosphosaccharomutase antibody
    • SA 36 antibody
    • SA-36 antibody
    • SA36 antibody
    • Sperm antigen 36 antibody
    see all

Images

  • Anti-Glucose 6 phosphate isomerase antibody (ab99189) at 0.3 µg/ml + Human heart lysate at 35 µg

    Developed using the ECL technique.

    Predicted band size: 63 kDa

References

ab99189 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Question
Answer


I am very pleased to hear you would like to accept our offer and test ab55973 in pig. This code will give you 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview forpig and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: https://www.abcam.com/Abreviews.

Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

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Answer

Thank you very much for your interest in our glucose 6 phospate isomerase antibodies.

We have two anti-G6P isomerase antibodies predicted to work in pig due to high immunogen sequence homology, but these haven't been tested in pig. They are ab99189 and ab55973.


https://www.abcam.com/Glucose-6-phosphate-isomerase-antibody-ab99189.html


https://www.abcam.com/Glucose-6-phosphate-isomerase-antibody-ab99189.html


To our knowledge, ab99189 and ab55973 havenot been tested in pig. Therefore, I can offer a discount off a future purchase if you buy ab99189 or ab55973 now, test it inpig and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of 1 free primary antibody.

If you are interested in this offer, please follow these steps:

1. Reply to this e-mail to let me know that you would like to proceed and test ab99189 or ab55973 in pig. I will then send a discount code. This code must be issued before purchasing ab99189 or ab55973 so please wait for my reply before ordering.

2. Purchase ab99189 or ab55973 either by phone, fax, or online (www.abcam.com).

3. Test it in pig.

4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews.

5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for anyprimary antibodyordered and the discount code is valid for 4 months after issue.

We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if ab99189 or ab55973 turns out to be unsuitable for pig, you will still receive the discount on your next purchase after your Abreview has been submitted.

Please let me know if you have any questions about this offer and I would be happy to help you further.

The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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