Product nameAnti-Glucose 6 phosphate isomerase antibody [EPR11663(B)]
See all Glucose 6 phosphate isomerase primary antibodies
DescriptionRabbit monoclonal [EPR11663(B)] to Glucose 6 phosphate isomerase
Tested applicationsSuitable for: WB, IHC-P, ICC/IF, Flow Cytmore details
Unsuitable for: IP
Species reactivityReacts with: Human
Synthetic peptide, corresponding to residues in Human Glucose 6 phosphate isomerase (UniProt: P06744).
- HepG2, HeLa, HT29, and A549 cell lysates; Human prostatic hyperplasia and Human thyroid carcinoma tissues; HepG2 and A549 cells.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
PurityTissue culture supernatant
- Anti-Glucose 6 phosphate isomerase antibody [EPR11663(B)] (Alexa Fluor® 647) (ab203975)
- Anti-Glucose 6 phosphate isomerase antibody [EPR11663(B)] (Alexa Fluor® 488) (ab204382)
- Anti-Glucose 6 phosphate isomerase antibody [EPR11663(B)] (Phycoerythrin) (ab210599)
- Anti-Glucose 6 phosphate isomerase antibody [EPR11663(B)] (Alexa Fluor® 568) (ab213349)
Our Abpromise guarantee covers the use of ab167394 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Predicted molecular weight: 63 kDa.|
|IHC-P||1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|ICC/IF||1/100 - 1/500.|
|Flow Cyt||1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
FunctionBesides it's role as a glycolytic enzyme, mammalian GPI can function as a tumor-secreted cytokine and an angiogenic factor (AMF) that stimulates endothelial cell motility. GPI is also a neurotrophic factor (Neuroleukin) for spinal and sensory neurons.
PathwayCarbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 2/4.
Involvement in diseaseDefects in GPI are the cause of hemolytic anemia non-spherocytic due to glucose phosphate isomerase deficiency (HA-GPID) [MIM:613470]. It is a form of anemia in which there is no abnormal hemoglobin or spherocytosis. It is caused by glucose phosphate isomerase deficiency. Severe GPI deficiency can be associated with hydrops fetalis, immediate neonatal death and neurological impairment.
Sequence similaritiesBelongs to the GPI family.
modificationsPhosphorylation at Ser-185 by CK2 has been shown to decrease enzymatic activity and may contribute to secretion by a non-classical secretory pathway.
Cellular localizationCytoplasm. Secreted.
- Information by UniProt
- AMF antibody
- Aurocrine motility factor antibody
- Autocrine motility factor antibody
Immunocytochemistry/Immunofluorescence analysis of MCF-7 (human breast carcinoma) labelling Glucose 6 phosphate isomerase with purified ab167394 at 1/500. Cells were fixed with 100% methanol. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody (Ab150077). Nuclei counterstained with DAPI (blue).
Control: PBS only
ab167394 showing +ve staining in Human normal brain.
ab167394 showing +ve staining in Human normal pancreas.
ab167394 showing +ve staining in Human ovarian carcinoma.
ab167394 showing +ve staining in Human skeletal muscle.
All lanes : Anti-Glucose 6 phosphate isomerase antibody [EPR11663(B)] (ab167394) at 1/1000 dilution
Lane 1 : HepG2 cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : HT29 cell lysate
Lane 4 : A549 cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 63 kDa
Immunofluorescent analysis of HepG2 cells labeling Glucose 6 phosphate isomerase with ab167394 at 1/100 dilution.
Immunohistochemical analysis of paraffin-embedded Human prostatic hyperplasia tissue, labeling Glucose 6 phosphate isomerase with ab167394 at 1/100 dilution.
Immunohistochemical analysis of paraffin-embedded Human thyroid carcinoma tissue labeling Glucose 6 phosphate isomerase with ab167394 at 1/100 dilution.
Flow cytometric analysis of permeabilized A549 cells, labeling Glucose 6 phosphate isomerase with ab167394 at 1/10 dilution.
ab167394 has not yet been referenced specifically in any publications.