Glucose Assay Kit (ab65333)
Key features and details
- Assay type: Quantitative
- Detection method: Colorimetric/Fluorometric
- Platform: Microplate reader
- Assay time: 40 min
- Sample type: Cell culture supernatant, Other biological fluids, Plasma, Serum, Urine
- Sensitivity: 1 µM
Overview
-
Product name
Glucose Assay Kit
See all Glucose kits -
Detection method
Colorimetric/Fluorometric -
Sample type
Cell culture supernatant, Urine, Serum, Plasma, Other biological fluids -
Assay type
Quantitative -
Sensitivity
1 µM -
Range
1 µM - 10000 µM -
Assay time
0h 40m -
Product overview
Glucose Assay Kit ab65333 is a rapid, simple and sensitive assay used to quantify glucose levels in biological samples such as serum, plasma, and other body fluids, food, growth medium, etc.
In the glucose assay protocol, the glucose enzyme mix oxidizes glucose to generate a product which reacts with a dye to generate color (λ = 570 nm) and fluorescence (Ex/Em = 535/587 nm). The generated color and fluorescence is proportionally to the amount of glucose.
The kit detects glucose in the range 1-10000 µM.
Glucose assay protocol summary:
- add samples (deproteinized) and standards to wells
- add reaction mix and incubate for 30 min at 37ºC
- analyze with microplate reader -
Notes
This product is manufactured by BioVision, an Abcam company and was previously called K606 Glucose Colorimetric/Fluorometric Assay Kit. K606-100 is the same size as the 100 test size of ab65333.
If you have reducing substances in your samples, we recommend using Glucose Detection Kit II (ab102517).
Review our Metabolism Assay Guide to learn about assays for metabolites, metabolic enzymes, mitochondrial function, and oxidative stress, and also about how to assay metabolic function in live cells using your plate reader.
-
Platform
Microplate reader
Properties
-
Storage instructions
Store at -20°C. Please refer to protocols. -
Components Identifier 100 tests 2000 tests Assay Buffer WM 1 x 25ml 20 x 25ml Glucose Enzyme Mix (lyophilized) Green 1 vial 20 vials Glucose Probe (in DMSO) Red 1 x 200µl 20 x 200µl Glucose Standard (100 nmol/µl ) Yellow 1 x 100µl 20 x 100µl -
Research areas
-
Relevance
Glucose (C6H12O6; FW: 180.16) is a ubiquitous energy source in most organisms, from bacteria to humans. The breakdown of carbohydrates produces mono- and disaccharides, most of which is glucose. Through glycolysis and TCA (citric acid cycle), glucose is oxidized to eventually form CO2 and water, generating the universal energy molecule ATP. Glucose is a primary source of energy for the brain and a critical component in the production of proteins and in lipid metabolism and therefore measurement of glucose level is a key diagnostic parameter for many metabolic disorders.
Images
-
Functional studies- ab65333Image from Shao W et al., PLoS One 7(1), Fig 2C. Doi: 10.1371/journal.pone.0028784. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/
Shao et al investigated the functional outcome of long- term curcumin supplementation on glucose homeostasis. Glucose metabolism was determined in animals with low fat diet (LFD), high fat diet (HFD) and HFD with curcumin feeding using ab65333. Intraperitoneal insulin tolerance tests(IPITT) were conducted at the end of the 26 weeks. It was concluded curcumin improves insulin sensitivity and disposal of glucose.
-
Standard curve: mean of duplicates (+/- SD) with background reads subtracted
-
Standard curve: mean of duplicates (+/- SD) with background reads subtracted
-
Glucose measured in cell lysates showing quantity (nmol) per million cells.
Samples with the concentration of 2x107 cells/mL were used. Samples were diluted 1.5-13.5 fold and measured colorimetrically.
-
Glucose measured in human biological fluids showing quantity (µmol) per mL of tested sample. Samples were diluted 13.5 fold and measured colorimetrically.
Datasheets and documents
-
SDS download
-
Datasheet download
References (88)
ab65333 has been referenced in 88 publications.
- Saw EL et al. Activation of the cardiac non-neuronal cholinergic system prevents the development of diabetes-associated cardiovascular complications. Cardiovasc Diabetol 20:50 (2021). PubMed: 33618724
- Santopaolo M et al. Activation of Bone Marrow Adaptive Immunity in Type 2 Diabetes: Rescue by Co-stimulation Modulator Abatacept. Front Immunol 12:609406 (2021). PubMed: 33746953
- Wang H et al. BCAT1 overexpression regulates proliferation and c-Myc/GLUT1 signaling in head and neck squamous cell carcinoma. Oncol Rep 45:N/A (2021). PubMed: 33760210
- Choksi A et al. Tumor suppressor SMAR1 regulates PKM alternative splicing by HDAC6-mediated deacetylation of PTBP1. Cancer Metab 9:16 (2021). PubMed: 33863392
- Wang Y & Liu S LncRNA GHET1 Promotes Hypoxia-Induced Glycolysis, Proliferation, and Invasion in Triple-Negative Breast Cancer Through the Hippo/YAP Signaling Pathway. Front Cell Dev Biol 9:643515 (2021). PubMed: 33869194