For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome
If you continue without changing your cookie settings, we'll assume you’re happy with this.
Inquiry: Dear Tech Support, I'm looking for the Abcam Glucose Assay Kit (ab65333). I've to use this kit to measure glucose consumption in various haematological cell lines and primary samples of AML and ALL. What are the concentrations that i should use in my experiments? 1x10^6 is sufficient or i need more cells?
Asked on Nov 09 2012
This kit would be used to measure glucose in the cell culture supernatant. The number of cells in culture would have an effect on the glucose consumption, but the amount of time the cells are cultured and the specific cell line you are testing for will also have an effect on glucose levels.
Therefore, it is difficult to provide precise details of the number of cells you should use. I can suggest growing the cells as normal, taking samples at various time points. In the protocol, we suggest testing several doses of your sample to make sure the readings are within the standard curve range. You may need to dilute them in order to ensure they are within range, in which case you will need to take this into account when calculating the glucose concentration from the reading obtained.
I am sorry it is difficult to be more precise than this for individual experiments, but I hope this will be helpful.
Answered on Nov 09 2012