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Ab: 65333: Wants to confirm that the minimum sample volume is .5 uL that have a minimum of 1uM
Ab 65336: What is the minimum and maximum volume of serum that can be tested directly.
Asked on Jan 25 2013
Thank you for your enquiry.
Answer 1: Yes, the minimal serum volume for this assay is 0.5 µl. The lower detection limit of this assay is 1 µM. That does not mean that you have to start with a particular conc of the sample. More specifically, you would not know the conc of glucose to begin with.
In the protocol step mammalian serum consists of 0.1-6 mM triglyceride. This is the conc of triglyceride, not serum. One can use a minimum of 1 µl and a max of 50 µl for this assay.
Answered on Jan 25 2013