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I want to measure glucose being released into media. How should the glucose contained in media be removed prior to the measurements?
Asked on Sep 06 2013
I suggest exchanging the medium before collecting samples. That is, culture the cells in medium with a glucose/energy source and then exchange the medium with glucose-free medium such as Krebs-Ringer-Phosphate-HEPES (KRPH) buffer for example, and then run the glucose assay using the KRPH buffer. All the glucose in the buffer will be from the cells. There might be need for stimulation of glucose transporter for the cells to release glucose into the buffer. I would strongly suggest referring to publications on these types of experiments for protocols.
Tom RuyleAbcam Scientific Support
Answered on Sep 06 2013