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I would like to confirm that this kit is not affected by the presence of phenol red in the media. I am using 1 ul of cell culture media with phenol red.
Asked on Mar 13 2014
The signal contributed by the phenol red in 1ul samples will be insignificant compared to the signal generated by the glucose in your samples. You should include a control consisting of 1ul of culture medium and subtract the absorbance or fluorometric value of that control from the values of your experimental samples.
Tom RuyleAbcam Scientific Support
Answered on Mar 13 2014