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Our samples are not compatible with the oxidase/peroxidase-based glucose assay ab65333. What is the principle of the ab102517 assay?
Asked on Apr 16 2014
The main difference between these two glucose assays is that in ab65333 the probe reacts with hydrogen peroxide and gets oxidized to produce colored compound detectable at 570nm or at Ex/Em 535/587nm. In ab102517, the probe reacts with NADH (produced during glucose dehydrogenation in the first step) and forms a colored product measured at 450nm.
Assay ab65333 can measure 1uM-10mM glucose. The additional sensitivity in ab65333 is achieved in the fluorometric measurement of the assay. Assay ab102517 can measure concentrations within the range of 20uM-10mM.
Tom RuyleAbcam Scientific Support
Answered on Apr 16 2014