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We need to measure free glucose in purified polysaccharide samples (bacterial derivation). Is kit ab65333 appropriate for use with our sample or will the enzyme break down larger polysaccharides, leading to false positive/high readings?
Asked on Apr 23 2014
We have not tested glucose in purified polysaccharide samples. The first step of the reaction is specific for glucose, so we expect this to work. It's possible that there will be some background, but this can be corrected for by subtracting the background control values (see page 8 of the protocol).
Caitlin Valued CustomerAbcam Scientific Support
Answered on Apr 23 2014