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I want to do the glucose test on plasma from infected patients. As our plate reader is outside the BSL, we're not allowed to take out material without inactivating it. Could you tell me whether it is possible to inactivate samples with H2SO4 or PFA before using the test or after the color change has taken place or whether this will interfere with the assay or measurement?
Asked on Aug 21 2014
I have been in contact with the lab and unfortunately, we have not tested whether the addition of acids such as H2SO4 or PFA will interfere with the assay.
They have suggested that in this situation, perhaps it is best if you deproteinise your samples to remove most proteins/enzymes which would constitute the “biohazard” in your samples.
Alternatively, would 10% SDS be suitable for inactivating your samples? Although we have not tested this, itght work since it has been used in other assays with similar chemistry.
Elisa ThomasAbcam Scientific Support
Answered on Aug 21 2014