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Signal Transduction Metabolism Energy Metabolism
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Glucose Assay Kit (Fluorometric, High Sensitivity) (ab169559)

  • Datasheet
  • SDS
  • Protocol Booklet
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Glucose standard curve
  • Measurement of glucose levels in samples

Key features and details

  • Assay type: Quantitative
  • Detection method: Fluorescent
  • Platform: Microplate reader
  • Sample type: Adherent cells, Other biological fluids, Plasma, Serum, Suspension cells, Tissue, Tissue Culture Media
  • Sensitivity: 0.5 µM

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Overview

  • Product name

    Glucose Assay Kit (Fluorometric, High Sensitivity)
    See all Glucose kits
  • Detection method

    Fluorescent
  • Sample type

    Serum, Plasma, Other biological fluids, Tissue, Adherent cells, Suspension cells, Tissue Culture Media
  • Assay type

    Quantitative
  • Sensitivity

    < 0.5 µM
  • Species reactivity

    Reacts with: Mammals, Other species
  • Product overview

    Picoprobe Glucose Assay Kit (ab169559) is a simple, rapid, ultra-sensitive assay to measure glucose. It is suitable for high-throughput use. In this assay, D-glucose is enzymatically oxidized to form a product which reacts with a colorless probe to generate the fluorescence (Ex/Em = 535/587 nm). The fluorescence generated is directly proportional to the amount of glucose. This assay kit can detect less than 0.5 µM glucose in various biological samples.

  • Notes

    Glucose is the main energy source for virtually all living organisms. Glucose level is a key diagnostic parameter for many metabolic disorders. Measurement of glucose can be very important in both research and drug discovery processes.

    Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
    It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

  • Platform

    Microplate reader

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components Identifier 100 tests
    Glucose Assay Buffer 1 x 25ml
    Glucose Enzyme Mix (lyophilized) Green 1 vial
    Glucose Standard Yellow 1 x 100µl
    Glucose Substrate Mix Red 1 vial
    PicoProbe Blue 1 x 0.4ml
  • Research areas

    • Signal Transduction
    • Metabolism
    • Energy Metabolism
    • Kits/ Lysates/ Other
    • Kits
    • Cell Metabolism Kits
    • Sugar Assays
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Energy transfer pathways
    • Energy Metabolism
  • Relevance

    Glucose (C6H12O6; FW: 180.16) is a ubiquitous energy source in most organisms, from bacteria to humans. The breakdown of carbohydrates produces mono- and disaccharides, most of which is glucose. Through glycolysis and TCA (citric acid cycle), glucose is oxidized to eventually form CO2 and water, generating the universal energy molecule ATP. Glucose is a primary source of energy for the brain and a critical component in the production of proteins and in lipid metabolism and therefore measurement of glucose level is a key diagnostic parameter for many metabolic disorders.

Associated products

    Images

    • Glucose standard curve
      Glucose standard curve
      Representative standard curve.
    • Measurement of glucose levels in samples
      Measurement of glucose levels in samples
      Measurement of glucose levels in human serum (1 µL of 1:10 diluted) and rat tissue lysates from liver, kidney and muscle (0.14 µg, 0.19 µg and 0.93 µg respectively).

    Protocols

    • Protocol Booklet

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
    • SDS
  • References (2)

    Publishing research using ab169559? Please let us know so that we can cite the reference in this datasheet.

    ab169559 has been referenced in 2 publications.

    • Schömel N  et al. UGCG influences glutamine metabolism of breast cancer cells. Sci Rep 9:15665 (2019). PubMed: 31666638
    • Wang L  et al. Arylamine N-acetyltransferase 1 protects against reactive oxygen species during glucose starvation: Role in the regulation of p53 stability. PLoS One 13:e0193560 (2018). PubMed: 29518119

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