Glucose Assay Kit (Fluorometric, High Sensitivity) (ab169559)
- Datasheet
- References (1)
- Protocols
Overview
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Product nameGlucose Assay Kit (Fluorometric, High Sensitivity)
See all Glucose kits -
Detection methodFluorescent
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Sample typeSerum, Plasma, Other biological fluids, Tissue, Adherent cells, Suspension cells, Tissue Culture Media
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Assay typeQuantitative
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Sensitivity< 0.5 µM
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Species reactivityReacts with: Other species, Mammals
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Product overview
Picoprobe Glucose Assay Kit (ab169559) is a simple, rapid, ultra-sensitive assay to measure glucose. It is suitable for high-throughput use. In this assay, D-glucose is enzymatically oxidized to form a product which reacts with a colorless probe to generate the fluorescence (Ex/Em = 535/587 nm). The fluorescence generated is directly proportional to the amount of glucose. This assay kit can detect less than 0.5 µM glucose in various biological samples.
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Notes
Glucose is the main energy source for virtually all living organisms. Glucose level is a key diagnostic parameter for many metabolic disorders. Measurement of glucose can be very important in both research and drug discovery processes.
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PlatformMicroplate reader
Properties
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Storage instructionsStore at -20°C. Please refer to protocols.
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Components Identifier 100 tests Glucose Assay Buffer 1 x 25ml Glucose Enzyme Mix (lyophilized) Green 1 vial Glucose Standard Yellow 1 x 100µl Glucose Substrate Mix Red 1 vial PicoProbe Blue 1 x 0.4ml -
Research areas
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RelevanceGlucose (C6H12O6; FW: 180.16) is a ubiquitous energy source in most organisms, from bacteria to humans. The breakdown of carbohydrates produces mono- and disaccharides, most of which is glucose. Through glycolysis and TCA (citric acid cycle), glucose is oxidized to eventually form CO2 and water, generating the universal energy molecule ATP. Glucose is a primary source of energy for the brain and a critical component in the production of proteins and in lipid metabolism and therefore measurement of glucose level is a key diagnostic parameter for many metabolic disorders.
Images
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Glucose standard curveRepresentative standard curve. -
Measurement of glucose levels in samplesMeasurement of glucose levels in human serum (1 µL of 1:10 diluted) and rat tissue lysates from liver, kidney and muscle (0.14 µg, 0.19 µg and 0.93 µg respectively).
Protocols
References
This product has been referenced in:
- Wang L et al. Arylamine N-acetyltransferase 1 protects against reactive oxygen species during glucose starvation: Role in the regulation of p53 stability. PLoS One 13:e0193560 (2018). Read more (PubMed: 29518119) »
