Serum, Plasma, Other biological fluids, Tissue, Adherent cells, Suspension cells, Tissue Culture Media
< 0.5 µM
Other species, Mammals
Picoprobe Glucose Assay Kit (ab169559) is a simple, rapid, ultra-sensitive assay to measure glucose. It is suitable for high-throughput use. In this assay, D-glucose is enzymatically oxidized to form a product which reacts with a colorless probe to generate the fluorescence (Ex/Em = 535/587 nm). The fluorescence generated is directly proportional to the amount of glucose. This assay kit can detect less than 0.5 µM glucose in various biological samples.
Glucose is the main energy source for virtually all living organisms. Glucose level is a key diagnostic parameter for many metabolic disorders. Measurement of glucose can be very important in both research and drug discovery processes.
Glucose (C6H12O6; FW: 180.16) is a ubiquitous energy source in most organisms, from bacteria to humans. The breakdown of carbohydrates produces mono- and disaccharides, most of which is glucose. Through glycolysis and TCA (citric acid cycle), glucose is oxidized to eventually form CO2 and water, generating the universal energy molecule ATP. Glucose is a primary source of energy for the brain and a critical component in the production of proteins and in lipid metabolism and therefore measurement of glucose level is a key diagnostic parameter for many metabolic disorders.
Glucose standard curve
Representative standard curve.
Measurement of glucose levels in samples
Measurement of glucose levels in human serum (1 µL of 1:10 diluted) and rat tissue lysates from liver, kidney and muscle (0.14 µg, 0.19 µg and 0.93 µg respectively).
Wang L et al. Arylamine N-acetyltransferase 1 protects against reactive oxygen species during glucose starvation: Role in the regulation of p53 stability. PLoS One13:e0193560 (2018).
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