Product nameGlucose Oxidase Activity Assay Kit (Colorimetric)
See all Glucose Oxidase kits
Sample typeCell Lysate
Assay typeEnzyme activity (quantitative)
Assay time0h 20m
Species reactivityReacts with: Other species, Mammals
Glucose Oxidase Activity Assay Kit ab219924 uses a simple colorimetric assay to measure glucose oxidase (GOx) activity in cell and tissue extracts, and biological fluids. The assay can be performed in a convenient 96-well or 384-well plate format and is easily adapted to automation without a separation or wash step.
The glucose oxidase assay protocol uses a red probe to monitor glucose oxidase activity, leading to an increase in absorbance that can be easily detected at OD575 nm.
Glucose oxidase assay protocol summary:
- add samples and standards to wells
- add glucose oxidase reaction mix
- analyze every 2-3 mins with a microplate reader in kinetic mode for at least 10-30 mins at 37ºC
Glucose oxidase (notatin, GOx, EC 220.127.116.11) is a dimeric enzyme that catalyzes oxidation of beta-D-glucose into hydrogen peroxide and D-glucono-1,5-lactone, which is hydrolyzed to gluconic acid.
Storage instructionsStore at -20°C. Please refer to protocols.
Components 500 tests Assay Buffer 1 x 50ml DMSO 1 x 200µl Glucose 1 vial Glucose Oxidase (100 U) 1 vial Horseradish Peroxidase (HRP) 1 vial Red Dye (light-sensitive) 1 vial
Glucose Oxidase Activity Assay Kit (Colorimetric) (ab219924). Typical glucose oxidase dose response curve. Absorbance was measured on a 96-well clear bottom plate using a SpectraMax microplate reader with path check on. As low as 12.5 mU/mL glucose oxidase activity can be detected after 30 minutes incubation.
ab219924 has not yet been referenced specifically in any publications.