Product nameAnti-Glucose Oxidase antibody
See all Glucose Oxidase primary antibodies
DescriptionGoat polyclonal to Glucose Oxidase
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Other species
Full length native protein (purified) corresponding to Glucose Oxidase aa 23-605. (Aspergillus niger).
SNGIEASLLTDPKDVSGRTVDYIIAGGGLTGLTTAARLTENPNISVLVIE SGSYESDRGPIIEDLNAYGDIFGSSVDHAYETVELATNNQTALIRSGNGL GGSTLVNGGTWTRPHKAQVDSWETVFGNEGWNWDNVAAYSLQAERARAPN AKQIAAGHYFNASCHGVNGTVHAGPRDTGDDYSPIVKALMSAVEDRGVPT KKDFGCGDPHGVSMFPNTLHEDQVRSDAAREWLLPNYQRPNLQVLTGQYV GKVLLSQNGTTPRAVGVEFGTHKGNTHNVYAKHEVLLAAGSAVSPTILEY SGIGMKSILEPLGIDTVVDLPVGLNLQDQTTATVRSRITSAGAGQGQAAW FATFNETFGDYSEKAHELLNTKLEQWAEEAVARGGFHNTTALLIQYENYR DWIVNHNVAYSELFLDTAGVASFDVWDLLPFTRGYVHILDKDPYLHHFAY DPQYFLNELDLLGQAAATQLARNISNSGAMQTYFAGETIPGDNLAYDADL SAWTEYIPYHFRPNYHGVGTCSMMPKEMGGVVDNAARVYGVQGLRVIDGS IPPTQMSSHVMTVFYAMALKISDAILEDYASMQ
Database link: P13006
- Purified Glucose Oxidase protein.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 0.27% Potassium phosphate, 0.88% Sodium chloride
Concentration information loading...
Purification notesab181638 is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
Our Abpromise guarantee covers the use of ab181638 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use at an assay dependent concentration.|
|WB||1/1000 - 1/5000. Predicted molecular weight: 66 kDa.|
RelevanceGlucose Oxidase is widely used for the determination of glucose in body fluids and in removing residual glucose or oxygen from foods and beverages. Glucose Oxidase producing moulds such as Aspergillus and Penicillium species are used for the biological production of gluconic acid. CATALYTIC ACTIVITY: Beta D glucose + O2 = D glucono 1,5 lactone + H2O2.
- Beta D Glucose Oxygen 1 Oxido Reductase antibody
- Glucose oxidase (Precursor) antibody
- Glucose Oxyhydrase antibody
- GOD antibody
All lanes : Anti-Glucose Oxidase antibody (ab181638) at 1/5000 dilution
Lanes 1 & 3 : Glucose Oxidase protein at 1 µg
Lanes 2 & 4 : Glucose Oxidase protein at 0.25 µg
All lanes : Dylight 649 conjugated Donkey anti Goat at 1/10000 dilution
Predicted band size: 66 kDa
Lane 1 + 2 under reducing and Lane 3 +4 under non-reducing conditions.
Reduced samples of purified Glucose Oxidase contained 4% BME and were boiled for 5 minutes.
Anti-Glucose Oxidase antibody (ab181638) at 1/1000 dilution + Glucose Oxidase protein at 0.05 µg
Peroxidase anti-goat secondary antibody at 1/40000 dilution
Predicted band size: 66 kDa
Observed band size: 65-70 kDa why is the actual band size different from the predicted?
ab181638 has not yet been referenced specifically in any publications.