Product nameAnti-Glucose Transporter GLUT1 antibody
See all Glucose Transporter GLUT1 primary antibodies
DescriptionRabbit polyclonal to Glucose Transporter GLUT1
Tested applicationsSuitable for: IHC-Fr, IHC-P, ICC/IF, ELISA, WB, IPmore details
Species reactivityReacts with: Mouse, Rat, Sheep, Human
Predicted to work with: Horse, Chicken, Cat, Pig, Chimpanzee, Monkey, Baboon
- WB: Human placenta tissue lysate. SW480 whole cell lysate. IHC-P: Human placenta tissue. ICC/IF: HeLa cells. IHC-P: Mammary glands taken from wild-type lactating mice.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferSupplied in antibody stabilization buffer with 0.02% azide.
Concentration information loading...
PurityImmunogen affinity purified
Purification notesPurified antibody was isolated from immobilized antigen sepharose affinity column.
Our Abpromise guarantee covers the use of ab14683 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|WB||1/2500. Detects a band of approximately 50 kDa (predicted molecular weight: 55 kDa). We suggest that samples should not be heated or frozen.|
|IP||Use at an assay dependent concentration.|
FunctionFacilitative glucose transporter. This isoform may be responsible for constitutive or basal glucose uptake. Has a very broad substrate specificity; can transport a wide range of aldoses including both pentoses and hexoses.
Tissue specificityExpressed at variable levels in many human tissues.
Involvement in diseaseDefects in SLC2A1 are the cause of glucose transporter type 1 deficiency syndrome (GLUT1DS) [MIM:606777]; also known as blood-brain barrier glucose transport defect. This disease causes a defect in glucose transport across the blood-brain barrier. It is characterized by infantile seizures, delayed development, and acquired microcephaly.
Defects in SLC2A1 are the cause of dystonia type 18 (DYT18) [MIM:612126]. DYT18 is an exercise-induced paroxysmal dystonia/dyskinesia. Dystonia is defined by the presence of sustained involuntary muscle contraction, often leading to abnormal postures. DYT18 is characterized by attacks of involuntary movements triggered by certain stimuli such as sudden movement or prolonged exercise. In some patients involuntary exertion-induced dystonic, choreoathetotic, and ballistic movements may be associated with macrocytic hemolytic anemia.
Sequence similaritiesBelongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family. Glucose transporter subfamily.
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
Cellular localizationCell membrane. Melanosome. Localizes primarily at the cell surface (By similarity). Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
- Information by UniProt
- Choreoathetosis/spasticity episodic (paroxysmal choreoathetosis/spasticity) antibody
- CSE antibody
- DYT17 antibody
All lanes : Anti-Glucose Transporter GLUT1 antibody (ab14683) at 1/2500 dilution
Lane 1 : Human placenta tissue lysate - total protein (ab29745)
Lane 2 : SW480 (Human colon adenocarcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Predicted band size: 55 kDa
Observed band size: 55 kDa
This antibody labels Glut 1 protein as a smear due to large amount of glycosylation on Glut 1 transporter. We observed that boiling and freezing the Glut 1 samples resulted in aggregated form of the protein and gives a smear form high 100kDa range to 45kDa with significant reduction in Glut 1 (44-47kDa) band. We suggest that samples should not be heated or frozen. Following extraction in lysis buffer, the proteins are then dissolved in SDS-PAGE sample buffer 2X and reduced in presence of 2.5-5% BME. All samples at this time will be heated 70-80°C for 3 minutes and aliquot and stored frozen till it is time for analysis. Just before analyses the samples much be thawed at room temp or at 37°C to dissolve any precipitated SDS in the sample. The remaining sample must not be frozen again, this freeze and next thaw cycle will make aggregate in some samples.
Human normal placenta. Staining is observed at the cell surface.
Left panel: Primary antibody at 2 ug/ml.
Right panel: Isotype control.
Sections were stained using an automated system DAKO Autostainer Plus , at room temperature: sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers citrate pH 6.1 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutess. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako envision flex amplification kit for rabbit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with haematoxylin and coverslipped under DePeX.
Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
ICC/IF image of ab14683 stained HeLa (Human epithelial cell line from cervix adenocarcinoma) cells. The cells were 4% formaldehyde fixed (10 minutes) and then incubated in 1% BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1 hour to permeabilize the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab14683, 5 µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1 hour. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1 hour. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43 µM.
Immunohistochemical analysis of mammary glands taken from wild-type or Erbb4-/- lactating mice, staining Glucose Transporter GLUT1 with ab14683.
This product has been referenced in:
- Ida-Yonemochi H et al. Extracellular enzymatically synthesized glycogen promotes osteogenesis by activating osteoblast differentiation via Akt/GSK-3ß signaling pathway. J Cell Physiol N/A:N/A (2019). Read more (PubMed: 30604872) »
- Sun K et al. Oxidized ATM-mediated glycolysis enhancement in breast cancer-associated fibroblasts contributes to tumor invasion through lactate as metabolic coupling. EBioMedicine 41:370-383 (2019). Read more (PubMed: 30799198) »