Overview

  • Product name
    Anti-Glucose Transporter GLUT1 antibody
    See all Glucose Transporter GLUT1 primary antibodies
  • Description
    Rabbit polyclonal to Glucose Transporter GLUT1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC-Fr, IHC-P, ICC/IF, ELISA, WB, IPmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Sheep, Human
    Predicted to work with: Horse, Chicken, Cat, Pig, Chimpanzee, Monkey, Baboon
  • Immunogen

    Synthetic peptide corresponding to Rat Glucose Transporter GLUT1 aa 481-492 (C terminal) (Cysteine residue).
    Sequence:

    [C]GLFHPLGADSQV


    Database link: P11167

  • Positive control
    • WB: Human placenta tissue lysate. SW480 whole cell lysate. IHC-P: Human placenta tissue. ICC/IF: HeLa cells. IHC-P: Mammary glands taken from wild-type lactating mice.

Properties

Applications

Our Abpromise guarantee covers the use of ab14683 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr 1/200.
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF 1/200.
ELISA 1/12000.
WB 1/2500. Detects a band of approximately 50 kDa (predicted molecular weight: 55 kDa). We suggest that samples should not be heated or frozen.
IP Use at an assay dependent concentration.

Target

  • Function
    Facilitative glucose transporter. This isoform may be responsible for constitutive or basal glucose uptake. Has a very broad substrate specificity; can transport a wide range of aldoses including both pentoses and hexoses.
  • Tissue specificity
    Expressed at variable levels in many human tissues.
  • Involvement in disease
    Defects in SLC2A1 are the cause of glucose transporter type 1 deficiency syndrome (GLUT1DS) [MIM:606777]; also known as blood-brain barrier glucose transport defect. This disease causes a defect in glucose transport across the blood-brain barrier. It is characterized by infantile seizures, delayed development, and acquired microcephaly.
    Defects in SLC2A1 are the cause of dystonia type 18 (DYT18) [MIM:612126]. DYT18 is an exercise-induced paroxysmal dystonia/dyskinesia. Dystonia is defined by the presence of sustained involuntary muscle contraction, often leading to abnormal postures. DYT18 is characterized by attacks of involuntary movements triggered by certain stimuli such as sudden movement or prolonged exercise. In some patients involuntary exertion-induced dystonic, choreoathetotic, and ballistic movements may be associated with macrocytic hemolytic anemia.
  • Sequence similarities
    Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family. Glucose transporter subfamily.
  • Post-translational
    modifications
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization
    Cell membrane. Melanosome. Localizes primarily at the cell surface (By similarity). Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt
  • Database links
  • Alternative names
    • Choreoathetosis/spasticity episodic (paroxysmal choreoathetosis/spasticity) antibody
    • CSE antibody
    • DYT17 antibody
    • DYT18 antibody
    • DYT9 antibody
    • EIG12 antibody
    • erythrocyte/brain antibody
    • Erythrocyte/hepatoma glucose transporter antibody
    • facilitated glucose transporter member 1 antibody
    • Glucose transporter 1 antibody
    • Glucose transporter type 1 antibody
    • Glucose transporter type 1, erythrocyte/brain antibody
    • GLUT antibody
    • GLUT-1 antibody
    • GLUT1 antibody
    • GLUT1DS antibody
    • GLUTB antibody
    • GT1 antibody
    • GTG1 antibody
    • Gtg3 antibody
    • GTR1_HUMAN antibody
    • HepG2 glucose transporter antibody
    • HTLVR antibody
    • Human T cell leukemia virus (I and II) receptor antibody
    • MGC141895 antibody
    • MGC141896 antibody
    • PED antibody
    • RATGTG1 antibody
    • Receptor for HTLV 1 and HTLV 2 antibody
    • SLC2A1 antibody
    • Solute carrier family 2 (facilitated glucose transporter), member 1 antibody
    • Solute carrier family 2 antibody
    • Solute carrier family 2, facilitated glucose transporter member 1 antibody
    see all

Images

  • All lanes : Anti-Glucose Transporter GLUT1 antibody (ab14683) at 1/2500 dilution

    Lane 1 : Human placenta tissue lysate - total protein (ab29745)
    Lane 2 : SW480 (Human colon adenocarcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Predicted band size: 55 kDa
    Observed band size: 55 kDa



    This antibody labels Glut 1 protein as a smear due to large amount of glycosylation on Glut 1 transporter. We observed that boiling and freezing the Glut 1 samples resulted in aggregated form of the protein and gives a smear form high 100kDa range to 45kDa with significant reduction in Glut 1 (44-47kDa) band. We suggest that samples should not be heated or frozen. Following extraction in lysis buffer, the proteins are then dissolved in SDS-PAGE sample buffer 2X and reduced in presence of 2.5-5% BME. All samples at this time will be heated 70-80°C for 3 minutes and aliquot and stored frozen till it is time for analysis. Just before analyses the samples much be thawed at room temp or at 37°C to dissolve any precipitated SDS in the sample. The remaining sample must not be frozen again, this freeze and next thaw cycle will make aggregate in some samples.
  • Human normal placenta. Staining is observed at the cell surface.

    Left panel: Primary antibody at 2 ug/ml.

    Right panel: Isotype control.

    Sections were stained using an automated system DAKO Autostainer Plus , at room temperature: sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers citrate pH 6.1 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutess. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako envision flex amplification kit for rabbit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with haematoxylin and coverslipped under DePeX.

    Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.

  • ICC/IF image of ab14683 stained HeLa (Human epithelial cell line from cervix adenocarcinoma) cells. The cells were 4% formaldehyde fixed (10 minutes) and then incubated in 1% BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1 hour to permeabilize the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab14683, 5 µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1 hour. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1 hour. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43 µM.

  • Immunohistochemical analysis of mammary glands taken from wild-type or Erbb4-/- lactating mice, staining Glucose Transporter GLUT1 with ab14683.

References

This product has been referenced in:
  • Zhou Y  et al. Selective deletion of glutamine synthetase in the mouse cerebral cortex induces glial dysfunction and vascular impairment that precede epilepsy and neurodegeneration. Neurochem Int N/A:N/A (2018). Read more (PubMed: 30053506) »
  • Hulley PA  et al. Hypoxia-inducible factor 1-alpha does not regulate osteoclastogenesis but enhances bone resorption activity via prolyl-4-hydroxylase 2. J Pathol 242:322-333 (2017). Read more (PubMed: 28418093) »
See all 35 Publications for this product

Customer reviews and Q&As

1-10 of 11 Abreviews or Q&A

Answer

Thank you for contacting us.

Could you please explain what type of actualized data you would be interested in to see?

All three antibodies has been tested in WB and gave satisfactory results which is why WB application is guaranteed. We have sold 100s of units of these products and any positive or negative data submitted to us by our customers, is published under Abreviews and scientific support enquiries. These customers have successfully used these antibodies in western blot.

There are also many customer who reported observing multiple bands in western blot which is why we have updated the antibody datasheet with general note. We predict the multiple band or smear is probably due to high glycosylation or aggregation of protein due to heating. Our recommendations for best result are - not to heat the protein in sample buffer, 60-70C for 15-20 minutes will be OK and de-glycosylation.

These antibodies are tested for satisfactory results however if you are not convinced after using these products we will either replace the antibody or will provide you full refund.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

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Answer

Thank you for contacting us.
We always recommend our customers trying different procedure to get excellent results with antibodies sin IHC-P. It is a tricky applications due to antigen retrieval method involved.
Though citrate buffer pH 6.0 would be best however Tris-EDTA buffer can also be tried.
Microwave antigen retrieval procedure with a incubation time for 5, 10 and 15 minutes would be good to get the optimized time.
We have many IHC detection kits available. You can select any of these.
e.g. ab64264, ab93697, ab94698, ab93705, ab93677, ab64261, an64260 etc.
I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.
Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

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Answer

I am so sorry for the long delay in our answer.

Indeed, the laboratory of one of the antibodies against GLUT1 was searching for an image, as they had performed the Western blot on erythrocyte ghost membranes about 10 years ago. However even after several reminders, they have not found it as is seems. I am very sorry for this. They were ableto confirm, that there will be a diffuseband on this sample.

I realize that you have might moved on meanwhile with your research. If however we could still be of any assistance, please do not hesitate to contact us again. Once more, I am really sorry for the delay in the answer.

I wish you a great week.

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Answer

I am very pleased to hear you would like to accept our offer and test ab14683 on chicken samples. This code will give you one free primary antibody before the expiration date. To redeem this offer, please submit an Abreview for chicken and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. Please remember that submission of the Abreview is sufficient for the discount code to become active.

For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.

We publish positive and negative Abreviews on our datasheets so please submit the results of your tests regardless of the outcome. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

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Answer

Thank you for contacting Abcam.
For ab14683, we had not actually tested this antibody on chicken tissue, but based on sequence homology we thought there was a chance it would, hence the reason we said that it was predicted to work with this species.
I was wondering if you also ran a sample from mouse, rat or human as a positive control, as if you didn’t get a signal from that tissue, then we would know that there is something wrong with the vial we sent you. If however you got a signal from the mouse, rat or human, then we would know that this antibody is not recognizing the chicken sample.
Also, I was wondering how long you incubated your primary antibody for, was it overnight at 4C.
Looking forward to your reply.

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Question
Answer

Thank you for your phone call yesterday and for your patience while I have been in touch with the lab about ab14683. Based on the immunogen sequence, the antibody is predicted to react with chicken samples. However, since this has not been tested yet, we are unable to guarantee this cross-reactivity. We do have a testing discount program if you would like to try this antibody in chicken samples. You can purchase ab14683 at full price, test it in chicken, then submit an Abreview with your data. In exchange, you will receive one free primary antibody on a future purchase within 4 months of purchasing ab14683. If you are interested in this program, I will be happy to send more information. Please let me know if you have any questions, or if there is anything that we can do for you.

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Answer

I have received the following recommendations from the laboratory: Extract cellular and membrane proteins in lysis buffer. The proteins were then dissolved in SDS-PAGE sample buffer 2X and reduced the in presence of 2.5-5% BME. All samples at this time will be heated 70-80oC for 3 minutes and aliquot and stored frozen till it is time for analysis. Just before analyses the samples much be thawed at room temp or at 370C to dissolve any precipitated SDS in the sample. The remaining sample must not be frozen again, this freeze and next thaw cycle will make aggregate in some samples. I hope this clarification helps.

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Answer

I have received confirmation from the laboratory that tested ab14683 that this antibody labels Glut 1 protein as a smear due to large amount of glycosylation on Glut 1 transporter. We have also seen that boiling and freezing the Glut 1 samples resulted in an aggregated form of the protein and gives a smear form high 100kDa range to 45kDa with significant reduction in Glut 1 (44-47kDa) band. Based on these observations they have indeed suggested that samples to be analyzed should not be heated and frozen. I will add this information to our datasheet and hope this will also help you.

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Answer

Thank you for your enquiry. Unfortunately, we are not specialists in this area and we do not want to recommend a product that is unsuitable for your customer's needs. We suggest that your customer refers to the latest literature in this field and also to the Abcam datasheets to see which one would be best for his research. Regarding the peptide sequence, I am sorry we do not have any available information on. However, I have managed to find some publications which might be useful to your customer. 1. Dong et al "A mouse model for Glut-1 haploinsufficiency" Human Molecular Genetics Advance Access originally published online on February 23, 2006 Human Molecular Genetics 2006 15(7):1169-1179 2. Sakai et. al., Differentitation of mesenchymal stem cells transplanted to a rabbit degenerative Disc model. potential and limitations for stem cell therapy in Disc Regeneration. Spine 30; 2379-2387; 2006. 3. Blaser et al. In Vitro Studies on the Signal Transduction of Thyroidal Uptake of 18F-FDG and 131I-Iodide. Journal of Nuclear Medicine Vol. 47 No. 8 1382-1388, 2006. I hope the information above will be useful. Please do not hesitate to contact me again in the future if you need anything further.

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1-10 of 11 Abreviews or Q&A

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