Anti-Glucose Transporter GLUT1 antibody (ab14683)
Key features and details
- Rabbit polyclonal to Glucose Transporter GLUT1
- Suitable for: IHC-P, ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
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Overview
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Product name
Anti-Glucose Transporter GLUT1 antibody
See all Glucose Transporter GLUT1 primary antibodies -
Description
Rabbit polyclonal to Glucose Transporter GLUT1 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, ICC/IF, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Horse, Chicken, Cat, Pig, Chimpanzee, Monkey, Baboon -
Immunogen
Synthetic peptide corresponding to Rat Glucose Transporter GLUT1 aa 481-492 (C terminal) (Cysteine residue).
Sequence:[C]GLFHPLGADSQV
Database link: P11167 -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
Purified antibody was isolated from immobilized antigen sepharose affinity column. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab14683 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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ICC/IF |
1/200.
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WB |
1/2500. Detects a band of approximately 50 kDa (predicted molecular weight: 55 kDa). We suggest that samples should not be heated or frozen.
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Notes |
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IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
ICC/IF
1/200. |
WB
1/2500. Detects a band of approximately 50 kDa (predicted molecular weight: 55 kDa). We suggest that samples should not be heated or frozen. |
Target
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Function
Facilitative glucose transporter. This isoform may be responsible for constitutive or basal glucose uptake. Has a very broad substrate specificity; can transport a wide range of aldoses including both pentoses and hexoses. -
Tissue specificity
Expressed at variable levels in many human tissues. -
Involvement in disease
Defects in SLC2A1 are the cause of glucose transporter type 1 deficiency syndrome (GLUT1DS) [MIM:606777]; also known as blood-brain barrier glucose transport defect. This disease causes a defect in glucose transport across the blood-brain barrier. It is characterized by infantile seizures, delayed development, and acquired microcephaly.
Defects in SLC2A1 are the cause of dystonia type 18 (DYT18) [MIM:612126]. DYT18 is an exercise-induced paroxysmal dystonia/dyskinesia. Dystonia is defined by the presence of sustained involuntary muscle contraction, often leading to abnormal postures. DYT18 is characterized by attacks of involuntary movements triggered by certain stimuli such as sudden movement or prolonged exercise. In some patients involuntary exertion-induced dystonic, choreoathetotic, and ballistic movements may be associated with macrocytic hemolytic anemia. -
Sequence similarities
Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family. Glucose transporter subfamily. -
Post-translational
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR. -
Cellular localization
Cell membrane. Melanosome. Localizes primarily at the cell surface (By similarity). Identified by mass spectrometry in melanosome fractions from stage I to stage IV. - Information by UniProt
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Database links
- Entrez Gene: 396130 Chicken
- Entrez Gene: 6513 Human
- Entrez Gene: 397404 Pig
- Omim: 138140 Human
- SwissProt: P46896 Chicken
- SwissProt: P11166 Human
- Unigene: 473721 Human
- Unigene: 721551 Human
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Alternative names
- Choreoathetosis/spasticity episodic (paroxysmal choreoathetosis/spasticity) antibody
- CSE antibody
- DYT17 antibody
see all
Images
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All lanes : Anti-Glucose Transporter GLUT1 antibody (ab14683) at 1/2500 dilution
Lane 1 : Human placenta tissue lysate - total protein (ab29745)
Lane 2 : SW480 (Human colon adenocarcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Predicted band size: 55 kDa
Observed band size: 55 kDa
This antibody labels Glut 1 protein as a smear due to large amount of glycosylation on Glut 1 transporter. We observed that boiling and freezing the Glut 1 samples resulted in aggregated form of the protein and gives a smear form high 100kDa range to 45kDa with significant reduction in Glut 1 (44-47kDa) band. We suggest that samples should not be heated or frozen. Following extraction in lysis buffer, the proteins are then dissolved in SDS-PAGE sample buffer 2X and reduced in presence of 2.5-5% BME. All samples at this time will be heated 70-80°C for 3 minutes and aliquot and stored frozen till it is time for analysis. Just before analyses the samples much be thawed at room temp or at 37°C to dissolve any precipitated SDS in the sample. The remaining sample must not be frozen again, this freeze and next thaw cycle will make aggregate in some samples. -
Human normal placenta. Staining is observed at the cell surface.
Left panel: Primary antibody at 2 ug/ml.
Right panel: Isotype control.
Sections were stained using an automated system DAKO Autostainer Plus , at room temperature: sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers citrate pH 6.1 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutess. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako envision flex amplification kit for rabbit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with haematoxylin and coverslipped under DePeX.
Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
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ICC/IF image of ab14683 stained HeLa (Human epithelial cell line from cervix adenocarcinoma) cells. The cells were 4% formaldehyde fixed (10 minutes) and then incubated in 1% BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1 hour to permeabilize the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab14683, 5 µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1 hour. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1 hour. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43 µM.
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Immunohistochemical analysis of mammary glands taken from wild-type or Erbb4-/- lactating mice, staining Glucose Transporter GLUT1 with ab14683.
Protocols
Datasheets and documents
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Datasheet download
References (60)
ab14683 has been referenced in 60 publications.
- Wu R et al. Knockdown of circular RNA tousled-like kinase 1 relieves ischemic stroke in middle cerebral artery occlusion mice and oxygen-glucose deprivation and reoxygenation-induced N2a cell damage. Bioengineered 13:3434-3449 (2022). PubMed: 35067172
- Choe YG et al. Pericyte Loss Leads to Capillary Stalling Through Increased Leukocyte-Endothelial Cell Interaction in the Brain. Front Cell Neurosci 16:848764 (2022). PubMed: 35360491
- Burgess ER et al. Increased Ascorbate Content of Glioblastoma Is Associated With a Suppressed Hypoxic Response and Improved Patient Survival. Front Oncol 12:829524 (2022). PubMed: 35419292
- Ding Y et al. Circular RNA midline-1 (circMID1) promotes proliferation, migration, invasion and glycolysis in prostate cancer. Bioengineered 13:6293-6308 (2022). WB ; Human . PubMed: 35212614
- Thamrongwaranggoon U et al. Aberrant GLUT1 Expression Is Associated With Carcinogenesis and Progression of Liver Fluke-associated Cholangiocarcinoma. In Vivo 35:267-274 (2021). PubMed: 33402473