Application
Western blot
Sample
Zebrafish Tissue lysate - whole (Retina)
Gel Running Conditions
Reduced Denaturing (12% gel)
Loading amount
20 µg
Specification
Retina
Blocking step
Licor Odyssey Blocking Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: Licor Odyssey Blocking Buffer
Dilution
1/8000
Secondary antibody
Dilution
1/5000
Name
Non-Abcam antibody was used: IRDye 800CW
Host species: Donkey
Clonality: Polyclonal
Conjugation: IRDye® 800CW
Host species: Donkey
Clonality: Polyclonal
Conjugation: IRDye® 800CW
Detection
Detection method
Licor Odyssey Clx
Negative control
Blank lanes
Exposure
15 minute(s) and 0 second(s)
Bands
Specific: 46 kDa
Positive control
Mouse retina
Additional data
Additional Notes
PVDF membrane
RIPA buffer w/ mixed phosphatase and protease inhibitors
Lane 1: Zebrafish retina (no signal)
Lane 2: Mouse retina
Abcam response
We would like to thank this researcher for submitting their review. This antibody had not been tested for cross reactivity with zebrafish and we were unsure of what the results would be. The reviewer received an AbTrial discount code for using the antibody in an untested species. The antibody did detect a band at the expected 40-60 kDa range for the mouse sample.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
Submitted Oct 04 2016