Application
Western blot
Sample
Mouse Cell lysate - whole cell (EMT6 (ATCC® CRL-2755™) cells)
Gel Running Conditions
Reduced Denaturing (4-20% Tris Glycin)
Loading amount
40 µg
Specification
EMT6 (ATCC® CRL-2755™) cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Other product details
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% milk in TBST
Dilution
1/10000
Secondary antibody
Dilution
1/20000
Name
Non-Abcam antibody was used: Anti-rabbit IgG, HRP-linked Antibody #7074
Host species: Goat
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
SuperSignal™ West Femto Maximum Sensitiv
Exposure
6 minute(s) and 0 second(s)
Bands
Specific: 54 kDa Non-specific: 250 kDa
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
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Submitted Jul 06 2020