Product nameAnti-Glucose Transporter GLUT1 antibody [EPR3915] - Low endotoxin, Azide free
See all Glucose Transporter GLUT1 primary antibodies
DescriptionRabbit monoclonal [EPR3915] to Glucose Transporter GLUT1 - Low endotoxin, Azide free
Tested applicationsSuitable for: WB, IHC-P, ICC/IF, Flow Cytmore details
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human Glucose Transporter GLUT1 aa 450-550. The exact sequence is proprietary.
- Jurkat, HepG2, 3T3L1, Mouse brain, Human fetal brain and Human fetal liver lysates; Human cervical carcinoma and colonic adenocarcinoma tissues.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Dissociation constant (KD)KD = 7.70 x 10 -12 M Learn more about KD
Storage bufferpH: 7.20
Concentration information loading...
PurityProtein A purified
- Anti-Glucose Transporter GLUT1 antibody [EPR3915] (ab115730)
- Anti-Glucose Transporter GLUT1 antibody [EPR3915] (Alexa Fluor® 647) (ab195020)
- Anti-Glucose Transporter GLUT1 antibody [EPR3915] (HRP) (ab195021)
- Anti-Glucose Transporter GLUT1 antibody [EPR3915] (Alexa Fluor® 488) (ab195359)
- Anti-Glucose Transporter GLUT1 antibody [EPR3915] (Alexa Fluor® 594) (ab206360)
- Anti-Glucose Transporter GLUT1 antibody [EPR3915] (Phycoerythrin) (ab209449)
- Anti-Glucose Transporter GLUT1 antibody [EPR3915] (Alexa Fluor® 405) (ab210438)
Our Abpromise guarantee covers the use of ab196357 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent concentration. Detects a band of approximately 40-60 kDa (predicted molecular weight: 54 kDa).
Please check the parent abID, ab115730, for more information on dilutions.
|IHC-P||Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|ICC/IF||Use at an assay dependent concentration.|
|Flow Cyt||Use at an assay dependent concentration.
ab199376 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
FunctionFacilitative glucose transporter. This isoform may be responsible for constitutive or basal glucose uptake. Has a very broad substrate specificity; can transport a wide range of aldoses including both pentoses and hexoses.
Tissue specificityExpressed at variable levels in many human tissues.
Involvement in diseaseDefects in SLC2A1 are the cause of glucose transporter type 1 deficiency syndrome (GLUT1DS) [MIM:606777]; also known as blood-brain barrier glucose transport defect. This disease causes a defect in glucose transport across the blood-brain barrier. It is characterized by infantile seizures, delayed development, and acquired microcephaly.
Defects in SLC2A1 are the cause of dystonia type 18 (DYT18) [MIM:612126]. DYT18 is an exercise-induced paroxysmal dystonia/dyskinesia. Dystonia is defined by the presence of sustained involuntary muscle contraction, often leading to abnormal postures. DYT18 is characterized by attacks of involuntary movements triggered by certain stimuli such as sudden movement or prolonged exercise. In some patients involuntary exertion-induced dystonic, choreoathetotic, and ballistic movements may be associated with macrocytic hemolytic anemia.
Sequence similaritiesBelongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family. Glucose transporter subfamily.
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
Cellular localizationCell membrane. Melanosome. Localizes primarily at the cell surface (By similarity). Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
- Information by UniProt
- Choreoathetosis/spasticity episodic (paroxysmal choreoathetosis/spasticity) antibody
- CSE antibody
- DYT17 antibody
All lanes : Anti-Glucose Transporter GLUT1 antibody [EPR3915] - Low endotoxin, Azide free (ab196357)
Lane 1 : HepG2 (human hepatocellular carcinoma) whole cell lysate
Lane 2 : HT-29 (Human colorectal adenocarcinoma epithelial cell) whole cell lysate
Lysates/proteins at 15 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051)
Predicted band size: 54 kDa
Observed band size: 40-60 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutes
Blocking buffer and concentration: 5% NFDM/TBST
Diluting buffer and concentration: 5% NFDM/TBST
ab196357 has not yet been referenced specifically in any publications.