Overview

  • Product name
    Anti-Glucose Transporter GLUT1 antibody [SPM498]
    See all Glucose Transporter GLUT1 primary antibodies
  • Description
    Mouse monoclonal [SPM498] to Glucose Transporter GLUT1
  • Host species
    Mouse
  • Tested applications
    Suitable for: Flow Cyt, ICC/IF, IHC-FoFr, WB, IHC-Fr, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Positive control
    • HepG2 cells. Esophagous and breast carcinoma.
  • General notes
    Previous lots of this antibody gave good results in WB as published in Abreviews. We however are observing multiple bands with recent lots. GLUT1 is a multi-pass membrane protein so we can recommend not boiling the samples in sample buffer. We will also welcome more feedback from successful users.

Properties

Applications

Our Abpromise guarantee covers the use of ab40084 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 1µg for 106 cells.

Methanol or paraformaldehyde fixed cells.

ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

ICC/IF Use a concentration of 1 - 5 µg/ml.
IHC-FoFr Use at an assay dependent concentration. PubMed: 21688176
WB 1/5000. Detects a band of approximately 55 kDa (predicted molecular weight: 54 kDa).

Previous lots of this antibody gave good results in WB as published in Abreviews. We however are observing multiple bands with recent lots. GLUT1 is a multi-pass membrane protein so we can recommend heating the samples 60 - 70C for 10 -15 minutes instead of boiling in sample buffer. We will welcome any feedback the successful users have

IHC-Fr 1/200.

(see Abreview).

IHC-P 1/200. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Target

  • Function
    Facilitative glucose transporter. This isoform may be responsible for constitutive or basal glucose uptake. Has a very broad substrate specificity; can transport a wide range of aldoses including both pentoses and hexoses.
  • Tissue specificity
    Expressed at variable levels in many human tissues.
  • Involvement in disease
    Defects in SLC2A1 are the cause of glucose transporter type 1 deficiency syndrome (GLUT1DS) [MIM:606777]; also known as blood-brain barrier glucose transport defect. This disease causes a defect in glucose transport across the blood-brain barrier. It is characterized by infantile seizures, delayed development, and acquired microcephaly.
    Defects in SLC2A1 are the cause of dystonia type 18 (DYT18) [MIM:612126]. DYT18 is an exercise-induced paroxysmal dystonia/dyskinesia. Dystonia is defined by the presence of sustained involuntary muscle contraction, often leading to abnormal postures. DYT18 is characterized by attacks of involuntary movements triggered by certain stimuli such as sudden movement or prolonged exercise. In some patients involuntary exertion-induced dystonic, choreoathetotic, and ballistic movements may be associated with macrocytic hemolytic anemia.
  • Sequence similarities
    Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family. Glucose transporter subfamily.
  • Post-translational
    modifications
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization
    Cell membrane. Melanosome. Localizes primarily at the cell surface (By similarity). Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt
  • Database links
  • Alternative names
    • Choreoathetosis/spasticity episodic (paroxysmal choreoathetosis/spasticity) antibody
    • CSE antibody
    • DYT17 antibody
    • DYT18 antibody
    • DYT9 antibody
    • EIG12 antibody
    • erythrocyte/brain antibody
    • Erythrocyte/hepatoma glucose transporter antibody
    • facilitated glucose transporter member 1 antibody
    • Glucose transporter 1 antibody
    • Glucose transporter type 1 antibody
    • Glucose transporter type 1, erythrocyte/brain antibody
    • GLUT antibody
    • GLUT-1 antibody
    • GLUT1 antibody
    • GLUT1DS antibody
    • GLUTB antibody
    • GT1 antibody
    • GTG1 antibody
    • Gtg3 antibody
    • GTR1_HUMAN antibody
    • HepG2 glucose transporter antibody
    • HTLVR antibody
    • Human T cell leukemia virus (I and II) receptor antibody
    • MGC141895 antibody
    • MGC141896 antibody
    • PED antibody
    • RATGTG1 antibody
    • Receptor for HTLV 1 and HTLV 2 antibody
    • SLC2A1 antibody
    • Solute carrier family 2 (facilitated glucose transporter), member 1 antibody
    • Solute carrier family 2 antibody
    • Solute carrier family 2, facilitated glucose transporter member 1 antibody
    see all

Images

  • Anti-Glucose Transporter GLUT1 antibody [SPM498] (ab40084) at 1/500 dilution + Whole cell lysate prepared from human MDA-MB 231 cells at 20 µg

    Secondary
    HRP conjugated sheep polyclonal to mouse IgG at 1/5000 dilution

    Developed using the ECL technique.

    Predicted band size: 54 kDa


    Exposure time: 2 minutes

    See Abreview

  • Immunohistochemical analysis of 10% buffered formalin-fixed paraffin-embedded human dermal carcinoma tissue sections, labelling GLUT1 with ab40084 at a dilution of 1/100 incubated for 12 hours at 4°C. Heat mediated antigen retrival was performed with 10mM sodium citrate buffer at pH 6.0. Blocking was with 5% serum incubated for 1 hour at 21°C. The secondary was a Donkey anti-mouse polyclonal Alexa Fluor® 647 conjugate at 1/200. Counterstaining is DAPI in blue against Nuclear DNA.

    See Abreview

  • Overlay histogram showing HeLa cells stained with ab40084 (red line). The cells were fixed with methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab40084, 1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/250 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min) used under the same conditions.


  • ICC/IF image of ab40084 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab40084, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • ab40084 at a 1:200 dilution staining Glucose Transporter GLUT1 in Human esophagous tissue.
  • ab40084 staining Glucose Transporter GLUT1 in mouse brain tissue sections by Immunohistochemistry (PFA perfusion fixed frozen sections). Tissue samples were fixed by perfusion with formaldehyde and blocked with 20% serum for 20 minutes at room temperature. The sample was incubated with primary antibody (1/200) . A Biotin-conjugated donkey anti-mouse polyclonal (1/200) was used as the secondary antibody.

    See Abreview

  • All lanes : Anti-Glucose Transporter GLUT1 antibody [SPM498] (ab40084)

    Lane 1 : Cell lysates prepared from NIH3T3 cells
    Lane 2 : Cell lysates prepared from MDA MB 231 cells
    Lane 3 : Cell lysates prepared from HeLa cells

    Predicted band size: 54 kDa



    4oC (1 freeze/thaw)

  • ab40084 staining Glucose Transporter GLUT1 in mouse smooth muscle tissue sections by IHC-Fr (Frozen sections). Tissue samples were fixed with acetone and blocked with 5% serum for 2 hours at 25°C. The sample was incubated with primary antibody (1/200 im PBS-Tween) at 25°C for 1 hour. An Alexa Fluor®594-conjugated Goat polyclonal to mouse IgG (1/250) was used as secondary antibody.

    See Abreview

  • ICC/IF image of ab40084 stained HepG2 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab40084, 5µg/ml) overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti-mouse IgG - H&L, pre-adsorbed (ab96879) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References

This product has been referenced in:
  • Kavanagh Williamson M  et al. Upregulation of Glucose Uptake and Hexokinase Activity of Primary Human CD4+ T Cells in Response to Infection with HIV-1. Viruses 10:N/A (2018). Read more (PubMed: 29518929) »
  • Cretella D  et al. The anti-tumor efficacy of CDK4/6 inhibition is enhanced by the combination with PI3K/AKT/mTOR inhibitors through impairment of glucose metabolism in TNBC cells. J Exp Clin Cancer Res 37:72 (2018). Read more (PubMed: 29587820) »
See all 66 Publications for this product

Customer reviews and Q&As

1-10 of 40 Abreviews or Q&A

Abcam has not validated the combination of species/application used in this Abreview.
Application
IHC - Wholemount
Sample
Mouse Tissue (small intestine organoids)
Specification
small intestine organoids

Abcam user community

Verified customer

Submitted Mar 24 2017

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Sheep Tissue sections (Artery)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10mM Sodium Citrate pH6
Permeabilization
No
Specification
Artery
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Fixative
10% buffered normal formalin

Abcam user community

Verified customer

Submitted Sep 12 2016

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Pig Tissue sections (Lung)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10mM Citrate pH6
Permeabilization
No
Specification
Lung
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Fixative
10% normal buffered formalin

Abcam user community

Verified customer

Submitted Sep 07 2016

Answer


I am sorry to confirm that the immunogen used to produce this antibody is located in the intracellular part of the protein and therefore permeabilisation is necessary.


Read More
Application
Western blot
Sample
Mouse Tissue lysate - whole (Pancreas)
Gel Running Conditions
Reduced Denaturing (10)
Loading amount
60 µg
Specification
Pancreas
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

Clara Lubeseder-Martellato

Verified customer

Submitted Jul 20 2016

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Dog Tissue sections (necrotic canine sarcoma)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris/EDTA pH 9
Permeabilization
No
Specification
necrotic canine sarcoma
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 15% · Temperature: 20°C
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted Dec 18 2015

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (melanoma cell line xenograft)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris/EDTA pH 9
Permeabilization
No
Specification
melanoma cell line xenograft
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 15% · Temperature: 20°C
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted Dec 18 2015

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (skin tumor)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10mM Sodium citrate buffer pH 6
Permeabilization
No
Specification
skin tumor
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Fixative
10% buffered formalin

Abcam user community

Verified customer

Submitted Sep 30 2015

Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (muscle)
Permeabilization
No
Specification
muscle
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Fixative
Acetone

Abcam user community

Verified customer

Submitted Sep 28 2015

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Tumor)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10mM Sodium Citrate pH6
Permeabilization
No
Specification
Tumor
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Fixative
10% buffered formalin

Abcam user community

Verified customer

Submitted Jul 31 2015

1-10 of 40 Abreviews or Q&A

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