Anti-Glucose Transporter GLUT2 antibody [5D1] (ab85715)
Key features and details
- Mouse monoclonal [5D1] to Glucose Transporter GLUT2
- Suitable for: IHC-P, Flow Cyt, WB
- Reacts with: Human
- Isotype: IgG2a
Overview
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Product name
Anti-Glucose Transporter GLUT2 antibody [5D1]
See all Glucose Transporter GLUT2 primary antibodies -
Description
Mouse monoclonal [5D1] to Glucose Transporter GLUT2 -
Host species
Mouse -
Specificity
ab85715 recognizes Glucose Transporter GLUT2 expression on hGlut2-transfected NS0 cells but not on control transfectants. Detects Glut2 on the surface of the Human intestinal cell line Caco2 in flow cytometry and on fixed cells in immunocytochemistry tests. Based on flow cytometric tests on transfected cells, this antibody has no cross-reactivity with hGlut1 or hGlut3. -
Tested applications
Suitable for: IHC-P, Flow Cyt, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Tissue, cells or virus corresponding to Human Glucose Transporter GLUT2. Recombinant protein corresponding to Met1-Val524 from human GLUT 2 expressed in NS0 cells.
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Positive control
- Liver tissue. Flow Cytometry: HepG2 cells
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.40
Constituents: PBS, 5% Trehalose -
Concentration information loading...
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Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
5D1 -
Isotype
IgG2a -
Research areas
Associated products
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab85715 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P | (1) |
Use a concentration of 10 µg/ml.
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Flow Cyt |
Use 0.5µg for 106 cells.
ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
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WB | (2) |
Use a concentration of 1 µg/ml. Detects a band of approximately 57 kDa (predicted molecular weight: 57 kDa).
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Notes |
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IHC-P
Use a concentration of 10 µg/ml. |
Flow Cyt
Use 0.5µg for 106 cells. ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 57 kDa (predicted molecular weight: 57 kDa). |
Target
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Function
Facilitative glucose transporter. This isoform likely mediates the bidirectional transfer of glucose across the plasma membrane of hepatocytes and is responsible for uptake of glucose by the beta cells; may comprise part of the glucose-sensing mechanism of the beta cell. May also participate with the Na(+)/glucose cotransporter in the transcellular transport of glucose in the small intestine and kidney. -
Tissue specificity
Liver, insulin-producing beta cell, small intestine and kidney. -
Involvement in disease
Fanconi-Bickel syndrome -
Sequence similarities
Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family. Glucose transporter subfamily. -
Post-translational
modificationsN-glycosylated; required for stability and retention at the cell surface of pancreatic beta cells. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 6514 Human
- Omim: 138160 Human
- SwissProt: P11168 Human
- Unigene: 167584 Human
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Alternative names
- liver antibody
- Glucose Transporter 2 antibody
- Glucose Transporter GLUT2 antibody
see all
Images
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Anti-Glucose Transporter GLUT2 antibody [5D1] (ab85715) at 1 µg/ml + Human pancreas tissue lysate - total protein (ab29816) at 10 µg
Secondary
Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 57 kDa
Observed band size: 57 kDa
Exposure time: 8 minutes -
ab85715, at 10 µg/ml, staining Glucose Transporter GLUT2 in formalin-fixed, paraffin-embedded human Liver tissue by Immunohistochemistry.
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Overlay histogram showing HepG2 cells stained with ab85715 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab85715, 0.5µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HepG2 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (9)
ab85715 has been referenced in 9 publications.
- Pittala S et al. The VDAC1-based R-Tf-D-LP4 Peptide as a Potential Treatment for Diabetes Mellitus. Cells 9:N/A (2020). PubMed: 32093016
- Rogers RC et al. Evidence that hindbrain astrocytes in the rat detect low glucose with a glucose transporter 2-phospholipase C-calcium release mechanism. Am J Physiol Regul Integr Comp Physiol 318:R38-R48 (2020). PubMed: 31596114
- Villalva-Pérez JM et al. Characterization of Huh7 cells after the induction of insulin resistance and post-treatment with metformin. Cytotechnology 72:499-511 (2020). PubMed: 32409919
- Fujino M et al. Expression of glucose transporter-1 is correlated with hypoxia-inducible factor 1a and malignant potential in pancreatic neuroendocrine tumors. Oncol Lett 12:3337-3343 (2016). IHC-P ; Human . PubMed: 27900001
- Nguyen NQ et al. Upregulation of intestinal glucose transporters after Roux-en-Y gastric bypass to prevent carbohydrate malabsorption. Obesity (Silver Spring) 22:2164-71 (2014). PubMed: 24990218
- Mano Y et al. Correlation between biological marker expression and fluorine-18 fluorodeoxyglucose uptake in hepatocellular carcinoma. Am J Clin Pathol 142:391-7 (2014). Human . PubMed: 25125631
- Kern M et al. Liver-restricted Repin1 deficiency improves whole-body insulin sensitivity, alters lipid metabolism, and causes secondary changes in adipose tissue in mice. Diabetes 63:3295-309 (2014). PubMed: 24760138
- Deane AM et al. The effects of critical illness on intestinal glucose sensing, transporters, and absorption. Crit Care Med 42:57-65 (2014). PubMed: 23963126
- Vargas N et al. TAT-Mediated Transduction of MafA Protein In Utero Results in Enhanced Pancreatic Insulin Expression and Changes in Islet Morphology. PLoS One 6:e22364 (2011). IHC-FoFr ; Mouse . PubMed: 21857924