Recombinant
RabMAb

Recombinant Anti-Glucose Transporter GLUT2 antibody [EPR22946-74] (ab234440)

Overview

  • Product name

    Anti-Glucose Transporter GLUT2 antibody [EPR22946-74]
    See all Glucose Transporter GLUT2 primary antibodies
  • Description

    Rabbit monoclonal [EPR22946-74] to Glucose Transporter GLUT2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Flow Cyt, IHC-Pmore details
    Unsuitable for: ICC/IF,IP or WB
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human Glucose Transporter GLUT2 aa 50-150. The exact sequence is proprietary.
    Database link: P11168

  • Positive control

    • IHC-P: Human liver, Human hepatocellular carcinoma and Human kidney tissues. FC: HepG2 cells.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab234440 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/500.

 

 

IHC-P 1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

 

 

  • Application notes
    Is unsuitable for ICC/IF,IP or WB.
  • Target

    • Function

      Facilitative glucose transporter. This isoform likely mediates the bidirectional transfer of glucose across the plasma membrane of hepatocytes and is responsible for uptake of glucose by the beta cells; may comprise part of the glucose-sensing mechanism of the beta cell. May also participate with the Na(+)/glucose cotransporter in the transcellular transport of glucose in the small intestine and kidney.
    • Tissue specificity

      Liver, insulin-producing beta cell, small intestine and kidney.
    • Involvement in disease

      Fanconi-Bickel syndrome
    • Sequence similarities

      Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family. Glucose transporter subfamily.
    • Post-translational
      modifications

      N-glycosylated; required for stability and retention at the cell surface of pancreatic beta cells.
    • Cellular localization

      Membrane.
    • Information by UniProt
    • Database links

    • Alternative names

      • liver antibody
      • Glucose Transporter 2 antibody
      • Glucose Transporter GLUT2 antibody
      • Glucose transporter type 2 antibody
      • Glucose transporter type 2 liver antibody
      • Glucose transporter, liver/islet antibody
      • GLUT-2 antibody
      • GLUT2 antibody
      • GTR2_HUMAN antibody
      • GTT2 antibody
      • SLC2A2 antibody
      • Solute carrier family 2 (facilitated glucose transporter) member 2 antibody
      • Solute carrier family 2 facilitated glucose transporter member 2 antibody
      • Solute carrier family 2, facilitated glucose transporter member 2 antibody
      see all

    Images

    • Flow cytometric analysis of HepG2 (Human hepatocellular carcinoma epithelial cell) cells labelling Glucose Transporter GLUT2 with ab234440 at 1/500 dilution (Red) as compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat anti rabbit IgG (Alexa Fluor® 647, ab150079) at 1/2000 dilution was used as the secondary antibody.

      Gated on viable cells.

    • Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling Glucose Transporter GLUT2 with ab234440 at 1/500 dilution (1.07 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on human renal tubules. The section was incubated with ab234440 for 30 mins at RT. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Secondary antibody only control/ Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    • Immunohistochemical analysis of paraffin-embedded Human hepatocellular carcinoma tissue labeling Glucose Transporter GLUT2 with ab234440 at 1/500 dilution (1.07 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Membranous staining on human hepatocellular carcinoma (PMID/ 30374065). The section was incubated with ab234440 for 30 mins at RT. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Secondary antibody only control/ Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    • Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling Glucose Transporter GLUT2 with ab234440 at 1/500 dilution (1.07 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Membranous staining on human liver (PMID/ 30374065). The section was incubated with ab234440 for 30 mins at RT. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Secondary antibody only control/ Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    References

    ab234440 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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