Anti-Glucose Transporter GLUT3 antibody (ab15311)

Abreviews (4)Q&A (6)Specific References (19)

Overview

  • Product name
    Anti-Glucose Transporter GLUT3 antibody
    See all Glucose Transporter GLUT3 primary antibodies
  • Description
    Rabbit polyclonal to Glucose Transporter GLUT3
  • Host species
    Rabbit
  • Tested applications
    Suitable for: ELISA, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Human Glucose Transporter GLUT3 (C terminal).

  • Positive control
    • IHC-P: Human placenta tissue. WB: U87 -MG cell lysate; Human brain lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab15311 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.
WB Use a concentration of 0.5 µg/ml. Predicted molecular weight: 60 kDa.
IHC-P 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function
    Facilitative glucose transporter. Probably a neuronal glucose transporter.
  • Tissue specificity
    Highly expressed in brain. Expressed in many tissues.
  • Sequence similarities
    Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family. Glucose transporter subfamily.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
    • Alternative names
      • brain antibody
      • FLJ90380 antibody
      • Glucose transporter type 3 antibody
      • Glucose transporter type 3 brain antibody
      • GLUT 3 antibody
      • GLUT-3 antibody
      • GLUT3 antibody
      • GTR3_HUMAN antibody
      • Slc2a3 antibody
      • Solute Carrier Family 2 (Facilitated Glucose Transporter) Member 3 antibody
      • Solute carrier family 2, facilitated glucose transporter member 3 antibody
      see all

    Images

    • Western blot - Anti-Glucose Transporter GLUT3 antibody (ab15311)
      Western blot - Anti-Glucose Transporter GLUT3 antibody (ab15311)Image courtesy of Georg Ziegler by Abreview.
      All lanes : Anti-Glucose Transporter GLUT3 antibody (ab15311) at 1/400 dilution

      Lane 1 : Lysate prepared from U87-MG cells
      Lanes 2-3 : Lysate prepared from human brain
      Lane 4 : Lysate prepared from lymphoblastoid cell line

      Lysates/proteins at 15 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit IgG-HRP at 1/7500 dilution

      Predicted band size: 60 kDa
      Observed band size: 48 kDa (why is the actual band size different from the predicted?)
      Additional bands at: 60 kDa (possible glycosylated form)

      See Abreview

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucose Transporter GLUT3 antibody (ab15311)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucose Transporter GLUT3 antibody (ab15311)

      Formalin-fixed, paraffin-embedded human placenta tissue stained for Glucose Transporter GLUT3 using ab15311 at 1/50 dilution in immunohistochemical analysis.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucose Transporter GLUT3 antibody (ab15311)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucose Transporter GLUT3 antibody (ab15311)
      ab15311 staining Glucose Transporter GLUT3 in human placenta by Immunohistochemistry (FFPE-sections).
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucose Transporter GLUT3 antibody (ab15311)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucose Transporter GLUT3 antibody (ab15311)This image is courtesy of an anonymous Abreview.

      Formaldehyde-fixed, paraffin-embedded human testis tissue stained for Glucose Transporter GLUT3 using ab15311 at 1/200 dilution in immunohistochemical analysis.

      See Abreview

    References

    This product has been referenced in:
    • Kuang R  et al. GLUT3 upregulation promotes metabolic reprogramming associated with antiangiogenic therapy resistance. JCI Insight 2:e88815 (2017). WB ; Human . Read more (PubMed: 28138554) »
    • Mai WX  et al. Cytoplasmic p53 couples oncogene-driven glucose metabolism to apoptosis and is a therapeutic target in glioblastoma. Nat Med 23:1342-1351 (2017). Read more (PubMed: 29035366) »

    See all 19 Publications for this product

    Publishing research using ab15311? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

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    10 reviews or Q&A

    10
    Votes: Highest First

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Glucose Transporter GLUT3 antibody

     Excellent
    Abreviews
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Testis)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Ventana CC2 (pH6)
    Permeabilization
    No
    Specification
    Testis
    Blocking step
    Ventana blocking agent as blocking agent for 4 minute(s) · Concentration: 100% · Temperature: 36°C
    Fixative
    Formaldehyde
    Read More
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    Username

    Abcam user community

    Verified customer

    Submitted Feb 17 2017

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

     Good
    Abreviews
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3%
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citrate buffer
    Sample
    Human Tissue sections (Endometriosis)
    Specification
    Endometriosis
    Permeabilization
    No
    Fixative
    Paraformaldehyde
    Read More
    abreview image
    Username

    Dr. Brett Mckinnon

    Verified customer

    Submitted Jun 10 2013

    Hi Thank you for your answer. Sadly, we have seen this exact pattern on 8 differents gels, with 20 sample, analysed at least twice. Our protocol are in french but briefly, we prepare cortex from rat brain on ice, homogenized them  in PBS 1X, spi...

    Read More

    Thank you for your reply and for these additional details.

    I can see no apparent reason why this product would be giving you this pattern with your rat cortex samples independent of age or treatment. It is especially strange to me that it is...

    Read More

    Hi I have a question about GLUT-3 ab15311 antibody (lot 789020). In a western blot for 50 ug rat cortex, we see the expected band at 45-48 kDa but we also see a strong band at 38-40 kDa. Some rats have only this band, other have the 45 kDa and o...

    Read More

    Thank you for contacting us.

    Your issue is very interesting as some animals because of the variablity between samples independent of age or treatment. Would you be able to provide your WB protocols and sample treatments? It may be an issue...

    Read More

    Ichh habe eine Frage bezüglich ab15311. Ich bekomme mit diesem GLUT3-Antikörper in all meinen humanen Lysaten eine klare Bande bei ca. 48 kDa und bin ziemlich sicher, dass diese Bande spezifisch ist für GLUT3, da auch in vielen anderen Publikationen GL...

    Read More

    Vielen Dank für Ihre Anfrage und für Ihre Geduld.

    Leider haben wir für die Antikörper ab15311 und ab53095 kein Blockpeptid, und können auch keines herstellen oder beschaffen, aber wir haben eines für den ab41525. Dieses hat die Katalognummer...

    Read More

    We received 4 vials of antibody today that are all at room temperature. I am concerned that these antibodies will not work as expected and I don't want to waste time/effort/reagents with these suspect antibodies. I would like replacements of each as so...

    Read More

    I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued free of charge replacements.

    To check the status of the order please contact our Customer Service team ...

    Read More

    Sehr geehrte Damen und Herren,
    Bitte teilen Sie uns die IgG Konzentrationen nachfolgender Antikörper Lots mit.













    VEGFA

    abcam

    ab14994

    ...

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    Vielen Dank für Ihre Anfrage. Es freut mich bestätigen zu können, dass beide Antikörper keine Lot-spezifische Konzentration aufweisen. ab14994 hat eine Konzentration von 1mg/ml und ab15311 hat eine Konzentration von 0.2mg/ml. Bitte folgen Sie diesen...

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    Western blot abreview for Anti-Glucose Transporter GLUT3 antibody

     Good
    Abreviews
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (U87-MG, human brain, lymphoblastoid cell-line)
    Loading amount
    15 µg
    Specification
    U87-MG, human brain, lymphoblastoid cell-line
    Gel Running Conditions
    Reduced Denaturing (4-12 % Bis-Tris Gel)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Read More
    abreview image
    Username

    Mr. Georg Ziegler

    Verified customer

    Submitted Jan 11 2012

    Western blot abreview for Anti-Glucose Transporter GLUT3 antibody

     Excellent
    Abreviews
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (breast cancer cell lines)
    Loading amount
    30 µg
    Specification
    breast cancer cell lines
    Gel Running Conditions
    Reduced Denaturing (10% gel + glycerol)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More
    abreview image
    Username

    Miss. Kristina Terrell

    Verified customer

    Submitted Aug 20 2010

    What is the homology between the immunogen of this antibody and the chicken sequence? The accession number of the chicken sequence is NP_990842.

    Thank you for your enquiry. I was able to obtain the immunogen sequence. This has been updated on the datasheet: SIEPAKETTTNV aa 485-496 of human Unfortunately, it appears there is no significant homology between the chicken sequence and the imm...

    Read More

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