Overview

  • Product name
    Anti-Glucose Transporter GLUT3 antibody
    See all Glucose Transporter GLUT3 primary antibodies
  • Description
    Rabbit polyclonal to Glucose Transporter GLUT3
  • Host species
    Rabbit
  • Tested applications
    Suitable for: ELISA, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Human Glucose Transporter GLUT3 (C terminal).

  • Positive control
    • IHC-P: Human placenta tissue. WB: U87 -MG cell lysate; Human brain lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab15311 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.
WB Use a concentration of 0.5 µg/ml. Predicted molecular weight: 60 kDa.
IHC-P 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function
    Facilitative glucose transporter. Probably a neuronal glucose transporter.
  • Tissue specificity
    Highly expressed in brain. Expressed in many tissues.
  • Sequence similarities
    Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family. Glucose transporter subfamily.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • brain antibody
    • FLJ90380 antibody
    • Glucose transporter type 3 antibody
    • Glucose transporter type 3 brain antibody
    • GLUT 3 antibody
    • GLUT-3 antibody
    • GLUT3 antibody
    • GTR3_HUMAN antibody
    • Slc2a3 antibody
    • Solute Carrier Family 2 (Facilitated Glucose Transporter) Member 3 antibody
    • Solute carrier family 2, facilitated glucose transporter member 3 antibody
    see all

Images

  • All lanes : Anti-Glucose Transporter GLUT3 antibody (ab15311) at 1/400 dilution

    Lane 1 : Lysate prepared from U87-MG cells
    Lanes 2-3 : Lysate prepared from human brain
    Lane 4 : Lysate prepared from lymphoblastoid cell line

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit IgG-HRP at 1/7500 dilution

    Predicted band size: 60 kDa
    Observed band size: 48 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 60 kDa (possible glycosylated form)

    See Abreview

  • Formalin-fixed, paraffin-embedded human placenta tissue stained for Glucose Transporter GLUT3 using ab15311 at 1/50 dilution in immunohistochemical analysis.

  • ab15311 staining Glucose Transporter GLUT3 in human placenta by Immunohistochemistry (FFPE-sections).
  • Formaldehyde-fixed, paraffin-embedded human testis tissue stained for Glucose Transporter GLUT3 using ab15311 at 1/200 dilution in immunohistochemical analysis.

    See Abreview

References

This product has been referenced in:
  • Kuang R  et al. GLUT3 upregulation promotes metabolic reprogramming associated with antiangiogenic therapy resistance. JCI Insight 2:e88815 (2017). WB ; Human . Read more (PubMed: 28138554) »
  • Mai WX  et al. Cytoplasmic p53 couples oncogene-driven glucose metabolism to apoptosis and is a therapeutic target in glioblastoma. Nat Med 23:1342-1351 (2017). Read more (PubMed: 29035366) »
See all 19 Publications for this product

Customer reviews and Q&As

1-10 of 10 Abreviews or Q&A

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Testis)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Ventana CC2 (pH6)
Permeabilization
No
Specification
Testis
Blocking step
Ventana blocking agent as blocking agent for 4 minute(s) · Concentration: 100% · Temperature: 36°C
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted Feb 17 2017

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3%
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate buffer
Sample
Human Tissue sections (Endometriosis)
Specification
Endometriosis
Permeabilization
No
Fixative
Paraformaldehyde

Dr. Brett Mckinnon

Verified customer

Submitted Jun 10 2013

Answer

Thank you for your reply and for these additional details.

I can see no apparent reason why this product would be giving you this pattern with your rat cortex samples independent of age or treatment. It is especially strange to me that it is working correctly in hippocampal tissues from the same animals. Have you directly compared these hippocampal samples to the cortical samples?

I look forward to your reply. Please let me know if you have any further questions.

Read More

Answer

Thank you for contacting us.

Your issue is very interesting as some animals because of the variablity between samples independent of age or treatment. Would you be able to provide your WB protocols and sample treatments? It may be an issue with the gel as the pattern looks similar to the image posted on our site but with lower weights, have you seen this response on multiple gels or just this once?



Our products are covered by Abpromise guarantee for a period of six months after delivery. Should this product not perform as indicated on the datasheet, we provide scientific support, replacement or refund. More information on our Abpromise may be found at the following link:

https://www.abcam.com/index.html?pageconfig=abpromise

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

Read More

Answer

Vielen Dank für Ihre Anfrage und für Ihre Geduld.

Leider haben wir für die Antikörper ab15311 und ab53095 kein Blockpeptid, und können auch keines herstellen oder beschaffen, aber wir haben eines für den ab41525. Dieses hat die Katalognummer ab105625

Click here (or use the following: https://www.abcam.com/index.html?datasheet=105625).

Als Carrier- Protein wurde KHL (Keyhole limpet hemocyanin) verwendet, welches laut BLAST keine Übereinstimmung mit der Immunogen- Sequenz aufweist. Wir sind daher genauso ratlos wie Sie bezüglich der Nebenbanden.

Ich hoffe, dies hilft Ihnen trotzdem weiter. Bitte zögern Sie nicht, sich wieder bei uns zu melden, falls Sie weitere Fragen haben.

Read More

Answer

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued free of charge replacements.

To check the status of the order please contact our Customer Service team and reference this number.

Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.

I wish you the best of luck with your research.

Read More

Answer

Vielen Dank für Ihre Anfrage. Es freut mich bestätigen zu können, dass beide Antikörper keine Lot-spezifische Konzentration aufweisen. ab14994 hat eine Konzentration von 1mg/ml und ab15311 hat eine Konzentration von 0.2mg/ml. Bitte folgen Sie diesen links um zum jeweiligen datenblatt zugelangen und für mehr Informationen zu diesen Antikörpern: Click here (or use the following: https://www.abcam.com/index.html?datasheet=14994). Click here (or use the following: https://www.abcam.com/index.html?datasheet=15311). Ich hoffe diese Information war hilfreich und wünsche Ihnen viel Erfolg mit Ihrer Forschung.

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (U87-MG, human brain, lymphoblastoid cell-line)
Loading amount
15 µg
Specification
U87-MG, human brain, lymphoblastoid cell-line
Gel Running Conditions
Reduced Denaturing (4-12 % Bis-Tris Gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

Mr. Georg Ziegler

Verified customer

Submitted Jan 11 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (breast cancer cell lines)
Loading amount
30 µg
Specification
breast cancer cell lines
Gel Running Conditions
Reduced Denaturing (10% gel + glycerol)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Miss. Kristina Terrell

Verified customer

Submitted Aug 20 2010

Answer

Thank you for your enquiry. I was able to obtain the immunogen sequence. This has been updated on the datasheet: SIEPAKETTTNV aa 485-496 of human Unfortunately, it appears there is no significant homology between the chicken sequence and the immunogen. I am sorry I could not be more helpful.

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up