Anti-Glucose Transporter GLUT3 antibody (ab15311)

Reviews (4)Q&A (6)References (21)

Overview

  • Product name
    Anti-Glucose Transporter GLUT3 antibody
    See all Glucose Transporter GLUT3 primary antibodies
  • Description
    Rabbit polyclonal to Glucose Transporter GLUT3
  • Host species
    Rabbit
  • Tested applications
    Suitable for: ELISA, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Rat, Human
  • Immunogen

    Synthetic peptide within Human Glucose Transporter GLUT3 aa 450 to the C-terminus (C terminal). The exact sequence is proprietary.
    Database link: P11169

  • Positive control
    • IHC-P: Human placenta tissue. WB: U87 -MG cell lysate; Human brain lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab15311 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.
WB Use a concentration of 0.5 µg/ml. Predicted molecular weight: 60 kDa.
IHC-P 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function
    Facilitative glucose transporter. Probably a neuronal glucose transporter.
  • Tissue specificity
    Highly expressed in brain. Expressed in many tissues.
  • Sequence similarities
    Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family. Glucose transporter subfamily.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
    • Alternative names
      • brain antibody
      • FLJ90380 antibody
      • Glucose transporter type 3 antibody
      • Glucose transporter type 3 brain antibody
      • GLUT 3 antibody
      • GLUT-3 antibody
      • GLUT3 antibody
      • GTR3_HUMAN antibody
      • Slc2a3 antibody
      • Solute Carrier Family 2 (Facilitated Glucose Transporter) Member 3 antibody
      • Solute carrier family 2, facilitated glucose transporter member 3 antibody
      see all

    Images

    • Western blot - Anti-Glucose Transporter GLUT3 antibody (ab15311)
      Western blot - Anti-Glucose Transporter GLUT3 antibody (ab15311)Image courtesy of Georg Ziegler by Abreview.
      All lanes : Anti-Glucose Transporter GLUT3 antibody (ab15311) at 1/400 dilution

      Lane 1 : Lysate prepared from U87-MG cells
      Lanes 2-3 : Lysate prepared from human brain
      Lane 4 : Lysate prepared from lymphoblastoid cell line

      Lysates/proteins at 15 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit IgG-HRP at 1/7500 dilution

      Predicted band size: 60 kDa
      Observed band size: 48 kDa
      why is the actual band size different from the predicted?
      Additional bands at: 60 kDa (possible glycosylated form)

      See Abreview

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucose Transporter GLUT3 antibody (ab15311)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucose Transporter GLUT3 antibody (ab15311)

      Formalin-fixed, paraffin-embedded human placenta tissue stained for Glucose Transporter GLUT3 using ab15311 at 1/50 dilution in immunohistochemical analysis.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucose Transporter GLUT3 antibody (ab15311)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucose Transporter GLUT3 antibody (ab15311)
      ab15311 staining Glucose Transporter GLUT3 in human placenta by Immunohistochemistry (FFPE-sections).
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucose Transporter GLUT3 antibody (ab15311)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucose Transporter GLUT3 antibody (ab15311)This image is courtesy of an anonymous Abreview.

      Formaldehyde-fixed, paraffin-embedded human testis tissue stained for Glucose Transporter GLUT3 using ab15311 at 1/200 dilution in immunohistochemical analysis.

      See Abreview

    References

    This product has been referenced in:
    • Kuang R  et al. GLUT3 upregulation promotes metabolic reprogramming associated with antiangiogenic therapy resistance. JCI Insight 2:e88815 (2017). WB ; Human . Read more (PubMed: 28138554) »
    • Mai WX  et al. Cytoplasmic p53 couples oncogene-driven glucose metabolism to apoptosis and is a therapeutic target in glioblastoma. Nat Med 23:1342-1351 (2017). Read more (PubMed: 29035366) »
    See all 21 Publications for this product

    Publishing research using ab15311? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

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    1-10 of 10 Abreviews or Q&A

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Glucose Transporter GLUT3 antibody

     Excellent
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Testis)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Ventana CC2 (pH6)
    Permeabilization
    No
    Specification
    Testis
    Blocking step
    Ventana blocking agent as blocking agent for 4 minute(s) · Concentration: 100% · Temperature: 36°C
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Feb 17 2017

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

     Good
    Abreviews
    Abcam guarantees this product to work in the species/application used in this Abreview.
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3%
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citrate buffer
    Sample
    Human Tissue sections (Endometriosis)
    Specification
    Endometriosis
    Permeabilization
    No
    Fixative
    Paraformaldehyde
    Read More

    Dr. Brett Mckinnon

    Verified customer

    Submitted Jun 10 2013

    Question

    Hi Thank you for your answer. Sadly, we have seen this exact pattern on 8 differents gels, with 20 sample, analysed at least twice. Our protocol are in french but briefly, we prepare cortex from rat brain on ice, homogenized them  in PBS 1X, spin 10 min 2500 rpm and resuspend in RIPA with anti-protease (cocktail sigma) Then, we did the western on a 12% separating gel, 4% stacking gel, we tried 20 ug, 50 ug and 100 ug of protein (as assayed by Bradford) We blocked overnight at 4 C with 5% milk in TBST (TBS + 1% tween 20) then incubate antibody GLUT3 (1:500) in TBST with 5% milk for 2h15, wahsing 3 times for 10 min then incubate with secondary antibody for 1h (1:10000 ). We used western lightning plus-ECL kit from perkin elmer to revelate. The protocol works for other antbody with the same sample (MCT-2, insulin receptor, b-actin). We also used the same membranes, stripped them and try with the other antibody and it was good. And GLUT-3 antibody also work with other kind of sample (hippocampus, dissected from the cortex of those same animals and prepared on the same day with the same solution as the total cortex). This is most strange. If you have any suggestion, we would be please to try something to resolve this

    Read More
    Answer

    Thank you for your reply and for these additional details.

    I can see no apparent reason why this product would be giving you this pattern with your rat cortex samples independent of age or treatment. It is especially strange to me that it is working correctly in hippocampal tissues from the same animals. Have you directly compared these hippocampal samples to the cortical samples?

    I look forward to your reply. Please let me know if you have any further questions.

    Read More

    Question

    Hi I have a question about GLUT-3 ab15311 antibody (lot 789020). In a western blot for 50 ug rat cortex, we see the expected band at 45-48 kDa but we also see a strong band at 38-40 kDa. Some rats have only this band, other have the 45 kDa and other have both (independant of the age or treatment of our rats). I saw in one of the abreviews that this band had been seen before but considered non specific. But in our case, it's so intense and clean, we don't know what to think of it.  We don't think glycosylation or phosphorylation will cause the band to be smaller (but we never know), as this been tested ? Thank you for your help.

    Read More
    Answer

    Thank you for contacting us.

    Your issue is very interesting as some animals because of the variablity between samples independent of age or treatment. Would you be able to provide your WB protocols and sample treatments? It may be an issue with the gel as the pattern looks similar to the image posted on our site but with lower weights, have you seen this response on multiple gels or just this once?



    Our products are covered by Abpromise guarantee for a period of six months after delivery. Should this product not perform as indicated on the datasheet, we provide scientific support, replacement or refund. More information on our Abpromise may be found at the following link:

    https://www.abcam.com/index.html?pageconfig=abpromise

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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    Read More

    Question

    Ichh habe eine Frage bezüglich ab15311. Ich bekomme mit diesem GLUT3-Antikörper in all meinen humanen Lysaten eine klare Bande bei ca. 48 kDa und bin ziemlich sicher, dass diese Bande spezifisch ist für GLUT3, da auch in vielen anderen Publikationen GLUT3 in diesem Bereich gefunden wird und ich auch mit ab53095 und ab41525 die gleiche Bande bekomme. Meine diesbezüglichen Ergebnisse habe ich bereits als Abbreview auf Ihrer Seite veröffentlicht.
    Zusätzlich detektiert ab15311 jedoch eine teilweise noch stärkere Bande bei ca. 38 kDa. Diese Bande taucht nicht auf in humanen Hirnlysaten, humanen Spermatozoenlysaten und humanen Lymphozytenlysaten, allerdings in sämtlichen Lysaten, die aus kultivierten Zellen gewonnen wurden (EBV-LCLs, U87-MG, SHSY-5Y, Fibroblasten), in denen die GLUT3-Expression eher niedriger zu sein scheint.
    Nun meine Frage: ist es möglich, dass das Immunogen von ab15311 an ein Carrierprotein gekoppelt ist und daraus die Bande bei 38 kDa resultiert? Bei ab53095 bekomme ich interessanterweise neben der vermutlich spezifischen 48 kDa Bande eine zusätzliche Bande bei 29 kDa, was mich darin bestätigt, dass diese Banden unspezifisch sind. Es wäre von Vorteil, wenn Sie ein Blocking-Peptid für ab15311 zur Verfügung stellen könnten.

    Read More
    Answer

    Vielen Dank für Ihre Anfrage und für Ihre Geduld.

    Leider haben wir für die Antikörper ab15311 und ab53095 kein Blockpeptid, und können auch keines herstellen oder beschaffen, aber wir haben eines für den ab41525. Dieses hat die Katalognummer ab105625

    Click here (or use the following: https://www.abcam.com/index.html?datasheet=105625).

    Als Carrier- Protein wurde KHL (Keyhole limpet hemocyanin) verwendet, welches laut BLAST keine Übereinstimmung mit der Immunogen- Sequenz aufweist. Wir sind daher genauso ratlos wie Sie bezüglich der Nebenbanden.

    Ich hoffe, dies hilft Ihnen trotzdem weiter. Bitte zögern Sie nicht, sich wieder bei uns zu melden, falls Sie weitere Fragen haben.

    Read More

    Question

    We received 4 vials of antibody today that are all at room temperature. I am concerned that these antibodies will not work as expected and I don't want to waste time/effort/reagents with these suspect antibodies. I would like replacements of each as soon as possible.

    Read More
    Answer

    I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued free of charge replacements.

    To check the status of the order please contact our Customer Service team and reference this number.

    Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.

    I wish you the best of luck with your research.

    Read More

    Question

    Sehr geehrte Damen und Herren,
    Bitte teilen Sie uns die IgG Konzentrationen nachfolgender Antikörper Lots mit.













    VEGFA

    abcam

    ab14994

    IgG1

    mouse

    mAb

    944661



    Glut3

    abcam

    ab15311

    IgG

    rabbit

    pAb

    GR-52148-1



    Glut3

    abcam

    ab15311

    IgG

    rabbit

    pAb

    GR62200-1




    Vielen Dank.


    Mit freundlichen Grüßen,

    Read More
    Answer

    Vielen Dank für Ihre Anfrage. Es freut mich bestätigen zu können, dass beide Antikörper keine Lot-spezifische Konzentration aufweisen. ab14994 hat eine Konzentration von 1mg/ml und ab15311 hat eine Konzentration von 0.2mg/ml. Bitte folgen Sie diesen links um zum jeweiligen datenblatt zugelangen und für mehr Informationen zu diesen Antikörpern: Click here (or use the following: https://www.abcam.com/index.html?datasheet=14994). Click here (or use the following: https://www.abcam.com/index.html?datasheet=15311). Ich hoffe diese Information war hilfreich und wünsche Ihnen viel Erfolg mit Ihrer Forschung.

    Read More

    Western blot abreview for Anti-Glucose Transporter GLUT3 antibody

     Good
    Abreviews
    Abcam guarantees this product to work in the species/application used in this Abreview.
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (U87-MG, human brain, lymphoblastoid cell-line)
    Loading amount
    15 µg
    Specification
    U87-MG, human brain, lymphoblastoid cell-line
    Gel Running Conditions
    Reduced Denaturing (4-12 % Bis-Tris Gel)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Read More

    Mr. Georg Ziegler

    Verified customer

    Submitted Jan 11 2012

    Western blot abreview for Anti-Glucose Transporter GLUT3 antibody

     Excellent
    Abreviews
    Abcam guarantees this product to work in the species/application used in this Abreview.
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (breast cancer cell lines)
    Loading amount
    30 µg
    Specification
    breast cancer cell lines
    Gel Running Conditions
    Reduced Denaturing (10% gel + glycerol)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Miss. Kristina Terrell

    Verified customer

    Submitted Aug 20 2010

    Question

    What is the homology between the immunogen of this antibody and the chicken sequence? The accession number of the chicken sequence is NP_990842.

    Read More
    Answer

    Thank you for your enquiry. I was able to obtain the immunogen sequence. This has been updated on the datasheet: SIEPAKETTTNV aa 485-496 of human Unfortunately, it appears there is no significant homology between the chicken sequence and the immunogen. I am sorry I could not be more helpful.

    Read More

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