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  1. Link

    glucose-transporter-glut3-antibody-ab15311.pdf

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Signal Transduction Metabolism Plasma Membrane Channels
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Anti-Glucose Transporter GLUT3 antibody (ab15311)

  • Datasheet
  • SDS
Reviews (4)Q&A (6)References (31)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucose Transporter GLUT3 antibody (ab15311)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucose Transporter GLUT3 antibody (ab15311)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucose Transporter GLUT3 antibody (ab15311)

Key features and details

  • Rabbit polyclonal to Glucose Transporter GLUT3
  • Suitable for: WB, ELISA, IHC-P
  • Reacts with: Rat, Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-Glucose Transporter GLUT3 antibody
    See all Glucose Transporter GLUT3 primary antibodies
  • Description

    Rabbit polyclonal to Glucose Transporter GLUT3
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ELISA, IHC-Pmore details
  • Species reactivity

    Reacts with: Rat, Human
  • Immunogen

    Synthetic peptide within Human Glucose Transporter GLUT3 aa 450 to the C-terminus (C terminal). The exact sequence is proprietary.
    Database link: P11169

  • General notes

    This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7.60
    Preservative: 0.1% Sodium azide
    Constituents: PBS, 1% BSA
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Metabolism
    • Plasma Membrane
    • Channels
    • Cell Biology
    • Other Antibodies
    • Other Antibodies
    • Cancer
    • Cancer Metabolism
    • Metabolic signaling pathway
    • Metabolism of carbohydrates
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Carbohydrate metabolism
    • Metabolism
    • Types of disease
    • Cancer

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant Human Glucose Transporter GLUT3 protein (ab152681)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab15311 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB (2)
Use at an assay dependent concentration.
ELISA
Use at an assay dependent concentration.
IHC-P (2)
1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Notes
WB
Use at an assay dependent concentration.
ELISA
Use at an assay dependent concentration.
IHC-P
1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Facilitative glucose transporter. Probably a neuronal glucose transporter.
  • Tissue specificity

    Highly expressed in brain. Expressed in many tissues.
  • Sequence similarities

    Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family. Glucose transporter subfamily.
  • Cellular localization

    Membrane.
  • Target information above from: UniProt accession P11169 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 6515 Human
    • Entrez Gene: 25551 Rat
    • Omim: 138170 Human
    • SwissProt: P11169 Human
    • SwissProt: Q07647 Rat
    • Unigene: 419240 Human
    • Unigene: 95055 Rat
    • Alternative names

      • brain antibody
      • FLJ90380 antibody
      • Glucose transporter type 3 antibody
      • Glucose transporter type 3 brain antibody
      • GLUT 3 antibody
      • GLUT-3 antibody
      • GLUT3 antibody
      • GTR3_HUMAN antibody
      • Slc2a3 antibody
      • Solute Carrier Family 2 (Facilitated Glucose Transporter) Member 3 antibody
      • Solute carrier family 2, facilitated glucose transporter member 3 antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucose Transporter GLUT3 antibody (ab15311)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucose Transporter GLUT3 antibody (ab15311)

      Formalin-fixed, paraffin-embedded human placenta tissue stained for Glucose Transporter GLUT3 using ab15311 at 1/50 dilution in immunohistochemical analysis.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucose Transporter GLUT3 antibody (ab15311)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucose Transporter GLUT3 antibody (ab15311)
      ab15311 staining Glucose Transporter GLUT3 in human placenta by Immunohistochemistry (FFPE-sections).
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucose Transporter GLUT3 antibody (ab15311)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucose Transporter GLUT3 antibody (ab15311)This image is courtesy of an anonymous Abreview.

      Formaldehyde-fixed, paraffin-embedded human testis tissue stained for Glucose Transporter GLUT3 using ab15311 at 1/200 dilution in immunohistochemical analysis.

      See Abreview

    Protocols

    • Immunohistochemistry protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (31)

    Publishing research using ab15311? Please let us know so that we can cite the reference in this datasheet.

    ab15311 has been referenced in 31 publications.

    • Crouse MS  et al. The effects of maternal nutrition during the first 50 d of gestation on the location and abundance of hexose and cationic amino acid transporters in beef heifer uteroplacental tissues. J Anim Sci 99:N/A (2021). PubMed: 33247721
    • Lauer V  et al. Hypoxia drives glucose transporter 3 expression through hypoxia-inducible transcription factor (HIF)-mediated induction of the long noncoding RNA NICI. J Biol Chem 295:4065-4078 (2020). PubMed: 31690629
    • Osumi H  et al. Tumor cell-derived angiopoietin-like protein 2 establishes a preference for glycolytic metabolism in lung cancer cells. Cancer Sci 111:1241-1253 (2020). PubMed: 32012400
    • James-Allan LB  et al. Changes in Placental Nutrient Transporter Protein Expression and Activity Across Gestation in Normal and Obese Women. Reprod Sci 27:1758-1769 (2020). PubMed: 32072607
    • Pilipenko V  et al. Neuroprotective potential of antihyperglycemic drug metformin in streptozocin-induced rat model of sporadic Alzheimer's disease. Eur J Pharmacol 881:173290 (2020). PubMed: 32580040
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review

    Filter by Application

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    1-4 of 4 Abreviews

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Glucose Transporter GLUT3 antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Testis)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Ventana CC2 (pH6)
    Permeabilization
    No
    Specification
    Testis
    Blocking step
    Ventana blocking agent as blocking agent for 4 minute(s) · Concentration: 100% · Temperature: 36°C
    Fixative
    Formaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Feb 17 2017

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3%
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citrate buffer
    Sample
    Human Tissue sections (Endometriosis)
    Specification
    Endometriosis
    Permeabilization
    No
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Dr. Brett Mckinnon

    Verified customer

    Submitted Jun 10 2013

    Western blot abreview for Anti-Glucose Transporter GLUT3 antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (U87-MG, human brain, lymphoblastoid cell-line)
    Loading amount
    15 µg
    Specification
    U87-MG, human brain, lymphoblastoid cell-line
    Gel Running Conditions
    Reduced Denaturing (4-12 % Bis-Tris Gel)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Mr. Georg Ziegler

    Verified customer

    Submitted Jan 11 2012

    Western blot abreview for Anti-Glucose Transporter GLUT3 antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (breast cancer cell lines)
    Loading amount
    30 µg
    Specification
    breast cancer cell lines
    Gel Running Conditions
    Reduced Denaturing (10% gel + glycerol)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Miss. Kristina Terrell

    Verified customer

    Submitted Aug 20 2010

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