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Our Abpromise guarantee covers the use of ab41525 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/8000. Detects a band of approximately 45-47 kDa.Can be blocked with Glucose Transporter GLUT3 peptide (ab105625). Detects a band of approximately 45-47 kDa using 10ug hippocampal plasma membranes as antigen. The samples MUST NOT be boiled before running the gel as highly hydrophobic membrane proteins, like glucose transporters, aggregate severely at high temperatures due to the hydrophobic effect, which increases as a direct function of temperature. If the samples are boiled, much of the transporter will aggregate so severely it doesn't enter the running gel and higher order oligomers will be observed.|
|IP||Use at an assay dependent concentration.
Use at an assay dependent dilution. 2µl ab41525 will immunoprecipitate 80-85% GLUT3 from 250µg rat solublized hippocampal membranes.
|Dot blot||1/25000. With solublized hippocampal plasma membranes.|
|IHC-FrFl||Use at an assay dependent concentration.|
|IHC-P||Use a concentration of 4 µg/ml.|
|ICC/IF||Use at an assay dependent concentration.|
10% SDS-PAGE gel.
Incubated with the primary antibody for 2 hours at room temperature followed by 3x10 minutes washes in TBST.
ab41525 at a 1/100 dilution staining Glucose Transporter GLUT3 in human PMN cells by Immunocytochemistry/ Immunofluorescence (PFA fixed), incubated for 4 hours at 37°C. Permeabilized using 0.1% Triton X-100 in 2% BSA for 15 minutes. Blocked with 2% BSA for 30 minutes at 22°C. Secondary used undiluted polyclonal goat anti-rabbit IgG (H+L) conjugated to Alexa Fluor® 568 (red). Counterstain DAPI (blue).
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