Overview

  • Product name
    Anti-Glucose Transporter GLUT3 antibody
    See all Glucose Transporter GLUT3 primary antibodies
  • Description
    Rabbit polyclonal to Glucose Transporter GLUT3
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IP, IHC-Fr, ELISA, Dot blot, IHC-FrFl, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Rat Glucose Transporter GLUT3 (C terminal). The exact sequence is proprietary.
    Database link: Q07647
    (Peptide available as ab105625)

  • Positive control
    • IHC-P: Human duodenum tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab41525 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/8000. Detects a band of approximately 45-47 kDa.Can be blocked with Glucose Transporter GLUT3 peptide (ab105625). Detects a band of approximately 45-47 kDa using 10ug hippocampal plasma membranes as antigen. The samples MUST NOT be boiled before running the gel as highly hydrophobic membrane proteins, like glucose transporters, aggregate severely at high temperatures due to the hydrophobic effect, which increases as a direct function of temperature. If the samples are boiled, much of the transporter will aggregate so severely it doesn't enter the running gel and higher order oligomers will be observed.
IP Use at an assay dependent concentration.

Use at an assay dependent dilution. 2µl ab41525 will immunoprecipitate 80-85% GLUT3 from 250µg rat solublized hippocampal membranes.

IHC-Fr 1/250.
ELISA 1/25000.
Dot blot 1/25000. With solublized hippocampal plasma membranes.
IHC-FrFl Use at an assay dependent concentration.
IHC-P Use a concentration of 4 µg/ml.
ICC/IF Use at an assay dependent concentration.

Target

Images

  • All lanes : Anti-Glucose Transporter GLUT3 antibody (ab41525) at 1/2000 dilution

    Lanes 1 & 4 & 7 & 10 : Sal 3 month cortical extract
    Lanes 2 & 5 & 8 & 11 : Glu 3 month cortical extract
    Lanes 3 & 9 & 12 : CB 3 month cortical extract
    Lane 6 : GB 3 month cortical extract

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Alkaline phosphatase-conjugated goat anti-rabbit IgG at 1/5000 dilution


    10% SDS-PAGE gel.

    Incubated with the primary antibody for 2 hours at room temperature followed by 3x10 minutes washes in TBST.

  • ab41525 staining GLUT3 in human duodenum.
    Left panel: with primary antibody at 4 ug/ml. Right panel: isotype control.
    Sections were stained using an automated system (DAKO Autostainer Plus), at room temperature: sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers EDTA pH 9.0. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
  • ab41525 at a 1/100 dilution staining Glucose Transporter GLUT3 in human PMN cells by Immunocytochemistry/ Immunofluorescence (PFA fixed), incubated for 4 hours at 37°C. Permeabilized using 0.1% Triton X-100 in 2% BSA for 15 minutes. Blocked with 2% BSA for 30 minutes at 22°C. Secondary used undiluted polyclonal goat anti-rabbit IgG (H+L) conjugated to Alexa Fluor® 568 (red). Counterstain DAPI (blue).

    See Abreview

References

This product has been referenced in:
  • Ioja S  et al. Nocturnal Hypoxia Improves Glucose Disposal, Decreases Mitochondrial Efficiency, and Increases Reactive Oxygen Species in the Muscle and Liver of C57BL/6J Mice Independent of Weight Change. Oxid Med Cell Longev 2018:9649608 (2018). Read more (PubMed: 29507654) »
  • Aboushousha T  et al. Immunohistochemical and Biochemical Expression Patterns of TTF-1, RAGE, GLUT-1 and SOX2 in HCV-Associated Hepatocellular Carcinomas Asian Pac J Cancer Prev 19:219-227 (2018). Read more (PubMed: 29373917) »
See all 24 Publications for this product

Customer reviews and Q&As

1-10 of 20 Abreviews or Q&A

Application
Western blot
Sample
Mouse Tissue lysate - whole (Brain, heart, liver, muscle, retina)
Gel Running Conditions
Reduced Denaturing (12% gel)
Loading amount
30 µg
Specification
Brain, heart, liver, muscle, retina
Blocking step
Licor Odyssey Blocking Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Aug 23 2016

Answer

Thank you for contacting us.

I am sorry that this antibody did not perform as stated on the datasheet. I have asked our accounting department to issue a credit note for you, which can be redeemed against the invoice of a future order by passing it on to your purchasing department. To avoid confusion, please ensure your accounts department is aware of how the credit note is being used. If you have questions on how to use the credit note, please contact our accounting department.

Our accounting department can be contacted by email at us.credits@abcam.com or by telephone using the information at the Contact Us link in the top right corner of our website. Please refer to the credit note ID in any correspondence with our accounting department.

The credit note ID is for your reference only and does not automatically guarantee the credit.

I hope this experience will not prevent you from purchasing other products from us in the future. Our Scientific Support team is always at your service, should you require further expert advice.

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Question
Answer

Thank you for contacting Abcam earlier today.

I am sorry about the issues you are having with ab41525 in western blot and as I mentioned before the antibody is covered under our Abpromise for six months and is guaranteed to work in western blot on human and mouse samples . If the protocol tips that we discussed do not prove to be successful, please let me know and I would be happy to either send a replacement antibody or to process a refund.

If there is anything else I can help with, do not hesitate to contact me.

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Application
Western blot
Sample
Mouse Cell lysate - whole cell (Isolated Hippocampal neurons (E16))
Loading amount
100000 cells
Specification
Isolated Hippocampal neurons (E16)
Gel Running Conditions
Reduced Denaturing (15% SDS-PAGE Tris-Glycine)
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C

Dr. Gregory O'Sullivan

Verified customer

Submitted Jun 14 2012

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunocytochemistry
Sample
Mouse Cultured Cells (RAW 264.7)
Specification
RAW 264.7
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.1% Triton-X100 in 2% BSA for 15min
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 22°C

Dr. Mahesh Shivananjappa

Verified customer

Submitted May 24 2012

Answer

Vielen Dank für Ihre Anfrage und für Ihre Geduld.

Leider haben wir für die Antikörper ab15311 und ab53095 kein Blockpeptid, und können auch keines herstellen oder beschaffen, aber wir haben eines für den ab41525. Dieses hat die Katalognummer ab105625

Click here (or use the following: https://www.abcam.com/index.html?datasheet=105625).

Als Carrier- Protein wurde KHL (Keyhole limpet hemocyanin) verwendet, welches laut BLAST keine Übereinstimmung mit der Immunogen- Sequenz aufweist. Wir sind daher genauso ratlos wie Sie bezüglich der Nebenbanden.

Ich hoffe, dies hilft Ihnen trotzdem weiter. Bitte zögern Sie nicht, sich wieder bei uns zu melden, falls Sie weitere Fragen haben.

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Question
Answer

Thank you for your participation in Abcam's post complaint survey.

I am sorry to hear that the replacement antibody ab68792 has not worked in mouse. Mouse is an untested species for this antibody and therefore, I had sent you also a discount code to be used on a future order, once you have submnitted your data in form of an Abreview. If you have not yet done, please submit your mouse data as Abreview (even if the data are negative), andyou can thenobtain a free antibody of your choice.
Please let me know if you need any help with this.

Also, if you'd like to send your protocol information to me, I can see if I can provide any troubleshooting advice to help overcome the problem.

If there is anything else I can do for you, or if you have further questions to any of our products (e.g. primary antibodies, lysates, secondary antibodies, kits, controls, gels, proteins/peptides, slides, biochemicals, conjugation kits), please let me know.

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Answer

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement with the order number xxx with ab53095.

To check the status of the order please contact our Customer Service team and reference this number.

Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.

I wish you the best of luck with your research.

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Answer

Thank you for your email.

The lot number for ab41525 you were using was: GR41399-4.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Answer

Thank you for your email.

Abreviews should reflect the customer's opinion. Thus, I will leave it up to you to include the competitor's antibody. To us, either way is fine. We do not edit reviews for such information, only for completeness so that other customers can understand the review.

If you feel this information about the competitor-antibody is important, please include it in the review.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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1-10 of 20 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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