Anti-Glucose Transporter GLUT3 antibody (ab41525)
Key features and details
- Rabbit polyclonal to Glucose Transporter GLUT3
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
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- Research with confidence – consistent and reproducible results with every batch
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- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-Glucose Transporter GLUT3 antibody
See all Glucose Transporter GLUT3 primary antibodies -
Description
Rabbit polyclonal to Glucose Transporter GLUT3 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Mouse Glucose Transporter GLUT3 (C terminal). The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: P32037 -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
Preservative: 0.02% Sodium azide
Antibody stabilization buffer. -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
Antigen-sepharose affinity column. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab41525 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (5) |
1/8000. Detects a band of approximately 45-47 kDa. Detects a band of approximately 45-47 kDa using 10ug hippocampal plasma membranes as antigen.
The samples MUST NOT be boiled before running the gel as highly hydrophobic membrane proteins, like glucose transporters, aggregate severely at high temperatures due to the hydrophobic effect, which increases as a direct function of temperature. If the samples are boiled, much of the transporter will aggregate so severely it doesn't enter the running gel and higher order oligomers will be observed.
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IHC-P |
Use a concentration of 4 µg/ml.
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Notes |
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WB
1/8000. Detects a band of approximately 45-47 kDa. Detects a band of approximately 45-47 kDa using 10ug hippocampal plasma membranes as antigen. The samples MUST NOT be boiled before running the gel as highly hydrophobic membrane proteins, like glucose transporters, aggregate severely at high temperatures due to the hydrophobic effect, which increases as a direct function of temperature. If the samples are boiled, much of the transporter will aggregate so severely it doesn't enter the running gel and higher order oligomers will be observed. |
IHC-P
Use a concentration of 4 µg/ml. |
Target
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Function
Facilitative glucose transporter. Probably a neuronal glucose transporter. -
Tissue specificity
Highly expressed in brain. Expressed in many tissues. -
Sequence similarities
Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family. Glucose transporter subfamily. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 6515 Human
- Omim: 138170 Human
- SwissProt: P11169 Human
- Unigene: 419240 Human
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Alternative names
- brain antibody
- FLJ90380 antibody
- Glucose transporter type 3 antibody
see all
Images
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All lanes : Anti-Glucose Transporter GLUT3 antibody (ab41525) at 1/2000 dilution
Lanes 1 & 4 & 7 & 10 : Sal 3 month cortical extract
Lanes 2 & 5 & 8 & 11 : Glu 3 month cortical extract
Lanes 3 & 9 & 12 : CB 3 month cortical extract
Lane 6 : GB 3 month cortical extract
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Alkaline phosphatase-conjugated goat anti-rabbit IgG at 1/5000 dilution10% SDS-PAGE gel.
Incubated with the primary antibody for 2 hours at room temperature followed by 3x10 minutes washes in TBST.
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ab41525 staining GLUT3 in human duodenum.
Left panel: with primary antibody at 4 ug/ml. Right panel: isotype control.
Sections were stained using an automated system (DAKO Autostainer Plus), at room temperature: sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers EDTA pH 9.0. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (57)
ab41525 has been referenced in 57 publications.
- Farhadi A et al. P38 initiates degeneration of midbrain GABAergic and glutamatergic neurons in diabetes models. Eur J Neurosci 56:3755-3778 (2022). PubMed: 35513862
- Liu X et al. β-Carotene regulates glucose transport and insulin resistance in gestational diabetes mellitus by increasing the expression of SHBG. Clin Exp Pharmacol Physiol 49:1307-1318 (2022). PubMed: 35986631
- Jiang Y et al. 18F-FDG PET as an imaging biomarker for the response to FGFR-targeted therapy of cancer cells via FGFR-initiated mTOR/HK2 axis. Theranostics 12:6395-6408 (2022). PubMed: 36168616
- Yang N et al. HBXIP drives metabolic reprogramming in hepatocellular carcinoma cells via METTL3-mediated m6A modification of HIF-1α. J Cell Physiol 236:3863-3880 (2021). PubMed: 33305825
- Qin G et al. Shen-Zhi-Ling oral liquid ameliorates cerebral glucose metabolism disorder in early AD via insulin signal transduction pathway in vivo and in vitro. Chin Med 16:128 (2021). PubMed: 34857022