Overview

  • Product name
    Anti-Glucose Transporter GLUT3 antibody
    See all Glucose Transporter GLUT3 primary antibodies
  • Description
    Rabbit polyclonal to Glucose Transporter GLUT3
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IP, IHC-Fr, ELISA, Dot blot, IHC-FrFl, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Rat Glucose Transporter GLUT3 (C terminal). The exact sequence is proprietary.
    Database link: Q07647
    (Peptide available as ab105625)

  • Positive control
    • IHC-P: Human duodenum tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab41525 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/8000. Detects a band of approximately 45-47 kDa.Can be blocked with Glucose Transporter GLUT3 peptide (ab105625). Detects a band of approximately 45-47 kDa using 10ug hippocampal plasma membranes as antigen. The samples MUST NOT be boiled before running the gel as highly hydrophobic membrane proteins, like glucose transporters, aggregate severely at high temperatures due to the hydrophobic effect, which increases as a direct function of temperature. If the samples are boiled, much of the transporter will aggregate so severely it doesn't enter the running gel and higher order oligomers will be observed.
IP Use at an assay dependent concentration.

Use at an assay dependent dilution. 2µl ab41525 will immunoprecipitate 80-85% GLUT3 from 250µg rat solublized hippocampal membranes.

IHC-Fr 1/250.
ELISA 1/25000.
Dot blot 1/25000. With solublized hippocampal plasma membranes.
IHC-FrFl Use at an assay dependent concentration.
IHC-P Use a concentration of 4 µg/ml.
ICC/IF Use at an assay dependent concentration.

Target

Images

  • All lanes : Anti-Glucose Transporter GLUT3 antibody (ab41525) at 1/2000 dilution

    Lanes 1 & 4 & 7 & 10 : Sal 3 month cortical extract
    Lanes 2 & 5 & 8 & 11 : Glu 3 month cortical extract
    Lanes 3 & 9 & 12 : CB 3 month cortical extract
    Lane 6 : GB 3 month cortical extract

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Alkaline phosphatase-conjugated goat anti-rabbit IgG at 1/5000 dilution


    10% SDS-PAGE gel.

    Incubated with the primary antibody for 2 hours at room temperature followed by 3x10 minutes washes in TBST.

  • ab41525 staining GLUT3 in human duodenum.
    Left panel: with primary antibody at 4 ug/ml. Right panel: isotype control.
    Sections were stained using an automated system (DAKO Autostainer Plus), at room temperature: sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers EDTA pH 9.0. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
  • ab41525 at a 1/100 dilution staining Glucose Transporter GLUT3 in human PMN cells by Immunocytochemistry/ Immunofluorescence (PFA fixed), incubated for 4 hours at 37°C. Permeabilized using 0.1% Triton X-100 in 2% BSA for 15 minutes. Blocked with 2% BSA for 30 minutes at 22°C. Secondary used undiluted polyclonal goat anti-rabbit IgG (H+L) conjugated to Alexa Fluor® 568 (red). Counterstain DAPI (blue).

    See Abreview

References

This product has been referenced in:
  • Ioja S  et al. Nocturnal Hypoxia Improves Glucose Disposal, Decreases Mitochondrial Efficiency, and Increases Reactive Oxygen Species in the Muscle and Liver of C57BL/6J Mice Independent of Weight Change. Oxid Med Cell Longev 2018:9649608 (2018). Read more (PubMed: 29507654) »
  • Aboushousha T  et al. Immunohistochemical and Biochemical Expression Patterns of TTF-1, RAGE, GLUT-1 and SOX2 in HCV-Associated Hepatocellular Carcinomas Asian Pac J Cancer Prev 19:219-227 (2018). Read more (PubMed: 29373917) »
See all 28 Publications for this product

Customer reviews and Q&As

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1-9 of 9 Abreviews

Application
Western blot
Sample
Mouse Tissue lysate - whole (Brain, heart, liver, muscle, retina)
Gel Running Conditions
Reduced Denaturing (12% gel)
Loading amount
30 µg
Specification
Brain, heart, liver, muscle, retina
Blocking step
Licor Odyssey Blocking Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Aug 23 2016

Application
Western blot
Sample
Mouse Cell lysate - whole cell (Isolated Hippocampal neurons (E16))
Loading amount
100000 cells
Specification
Isolated Hippocampal neurons (E16)
Gel Running Conditions
Reduced Denaturing (15% SDS-PAGE Tris-Glycine)
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C

Dr. Gregory O'Sullivan

Verified customer

Submitted Jun 14 2012

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunocytochemistry
Sample
Mouse Cultured Cells (RAW 264.7)
Specification
RAW 264.7
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.1% Triton-X100 in 2% BSA for 15min
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 22°C

Dr. Mahesh Shivananjappa

Verified customer

Submitted May 24 2012

Application
Western blot
Sample
Human Cell lysate - whole cell (U87-MG, human brain lysates, human lymphoblasts)
Loading amount
15 µg
Specification
U87-MG, human brain lysates, human lymphoblasts
Gel Running Conditions
Reduced Denaturing (4-12 % Bis-Tris-Gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

Mr. Georg Ziegler

Verified customer

Submitted Jan 11 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Tissue lysate - whole (multiple tissues)
Loading amount
40 µg
Specification
multiple tissues
Gel Running Conditions
Reduced Denaturing (4-12% gradient)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Dr. Kris Frese

Verified customer

Submitted Jan 06 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (Platelets)
Loading amount
20 µg
Specification
Platelets
Treatment
ADP for 30 min
Gel Running Conditions
Reduced Denaturing (4-20%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

Dr. Mahesh Shivananjappa

Verified customer

Submitted Nov 22 2011

Application
Immunohistochemistry free floating
Sample
Meriones unguiculatus Tissue sections (Brain)
Specification
Brain

Abcam user community

Verified customer

Submitted Aug 04 2011

Application
Flow Cytometry
Sample
Human Cell (Platelets)
Specification
Platelets
Preparation
Cell harvesting/tissue preparation method: PL were isolated spinning Platelet rich plasma on Histopaque
Sample buffer: PBS
Fixation
Paraformaldehyde
Permeabilization
Yes - 0.1% Triton-X100 in 2% BSA for 30min
Gating Strategy
Platelets

Dr. Mahesh Shivananjappa

Verified customer

Submitted Dec 30 2010

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (PMN)
Specification
PMN
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.1% TritonX-100 in 2% BSA for 15 min
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 2% · Temperature: 22°C

Dr. Mahesh Shivananjappa

Verified customer

Submitted Dec 22 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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