Recombinant
RabMAb

Recombinant Anti-Glucose Transporter GLUT3 antibody [EPR10508(N)] - N-terminal (ab191071)

Overview

  • Product name

    Anti-Glucose Transporter GLUT3 antibody [EPR10508(N)] - N-terminal
    See all Glucose Transporter GLUT3 primary antibodies
  • Description

    Rabbit monoclonal [EPR10508(N)] to Glucose Transporter GLUT3 - N-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human Glucose Transporter GLUT3 aa 1-100 (N terminal). The exact sequence is proprietary.
    Database link: P11169

  • Positive control

    • WB: Mouse brain, heart, kidney and spleen tissue lysates; Rat brain, heart, kidney and spleen tissue lysates; SHSY-5Y, U87-MG, NIH3T3, HepG2, C6 and RAW264.7 cell lysates.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab191071 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 54 kDa (predicted molecular weight: 54 kDa).

Target

Images

  • All lanes : Anti-Glucose Transporter GLUT3 antibody [EPR10508(N)] - N-terminal (ab191071) at 1/1000 dilution

    Lane 1 : Mouse brain tissue lysates
    Lane 2 : Mouse heart tissue lysates
    Lane 3 : Mouse kidney tissue lysates
    Lane 4 : Mouse spleen tissue lysates
    Lane 5 : Rat brain tissue lysates
    Lane 6 : Rat heart tissue lysates
    Lane 7 : Rat kidney tissue lysates
    Lane 8 : Rat spleen tissue lysates

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 54 kDa
    Observed band size: 54 kDa


    Exposure time: 15 seconds


    Blocking/Diluting buffer and concentration: 5% NFDM/TBST

    We are unsure as to the identity of the band below 54kda. 

  • All lanes : Anti-Glucose Transporter GLUT3 antibody [EPR10508(N)] - N-terminal (ab191071) at 1/10000 dilution

    Lane 1 : SH-SHY5Y cell lysate
    Lane 2 : U87-MG cell lysate
    Lane 3 : HepG2 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 54 kDa
    Observed band size: 54 kDa

  • All lanes : Anti-Glucose Transporter GLUT3 antibody [EPR10508(N)] - N-terminal (ab191071) at 1/1000 dilution

    Lane 1 : C6 cell lysate
    Lane 2 : RAW264.7 cell lysate
    Lane 3 : NIH 3T3 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 54 kDa
    Observed band size: 54 kDa

References

This product has been referenced in:

  • Oliveira H  et al. GLUT1 and GLUT3 involvement in anthocyanin gastric transport- Nanobased targeted approach. Sci Rep 9:789 (2019). Read more (PubMed: 30692585) »
  • van der Kooij MA  et al. Chronic social stress-induced hyperglycemia in mice couples individual stress susceptibility to impaired spatial memory. Proc Natl Acad Sci U S A 115:E10187-E10196 (2018). Read more (PubMed: 30301805) »
See all 7 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Application
Western blot
Sample
Mouse Tissue lysate - whole (Brain)
Gel Running Conditions
Reduced Denaturing
Loading amount
20 µg
Specification
Brain
Blocking step
BSA as blocking agent for 10 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 4°C

Abcam user community

Verified customer

Submitted Jul 20 2018

Application
Western blot
Sample
Dog Cell lysate - whole cell (melanoma cell line)
Gel Running Conditions
Reduced Denaturing (12%)
Loading amount
10 µg
Specification
melanoma cell line
Blocking step
BlockAce as blocking agent for 50 minute(s) · Concentration: 100% · Temperature: 25°C

Dr. 令 中野

Verified customer

Submitted Nov 06 2017

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