Anti-Glucose Transporter GLUT4 antibody [1F8] (ab35826)


  • Product name
    Anti-Glucose Transporter GLUT4 antibody [1F8]
    See all Glucose Transporter GLUT4 primary antibodies
  • Description
    Mouse monoclonal [1F8] to Glucose Transporter GLUT4
  • Host species
  • Specificity
    This antibody is specific for the fat/muscle specific isoform of the Glucose Transporter GLUT4. We have data to indicate that this antibody may not cross react with Dog. However, this has not been conclusively tested and expression levels may vary in certain cell lines/tissues.
  • Tested applications
    Suitable for: ICC/IF, IP, IHC-Fr, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Rabbit, Human, Monkey
  • Immunogen

    Rat Glucose Transporter GLUT4 from partially purified vesicles.

  • Epitope
    This antibody has been shown to recognise an epitope in the cytoplasmic portion of the molecule.



Our Abpromise guarantee covers the use of ab35826 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 2.5 - 5 µg/ml.
IP Use at an assay dependent concentration. Membrane proteins at a concentration of 1-2 mg/ml can be immunoadsorbed using this antibody coupled with agarose beads.
IHC-Fr Use at an assay dependent concentration. PubMed: 20418694
WB Use at an assay dependent concentration. Predicted molecular weight: 56 kDa. 5 µg/ml of antibody will detect Glucose Transporter protein contained in 50-200 µg of solubilised membrane.


  • Function
    Insulin-regulated facilitative glucose transporter.
  • Tissue specificity
    Skeletal and cardiac muscles; brown and white fat.
  • Involvement in disease
    Diabetes mellitus, non-insulin-dependent
  • Sequence similarities
    Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family. Glucose transporter subfamily.
  • Post-translational
  • Cellular localization
    Cell membrane. Endomembrane system. Cytoplasm, perinuclear region. Localizes primarily to the perinuclear region, undergoing continued recycling to the plasma membrane where it is rapidly reinternalized. The dileucine internalization motif is critical for intracellular sequestration.
  • Information by UniProt
  • Database links
  • Alternative names
    • insulin-responsive antibody
    • Glucose transporter GLUT 4 antibody
    • Glucose transporter type 4 antibody
    • Glucose transporter type 4 insulin responsive antibody
    • GLUT 4 antibody
    • GLUT-4 antibody
    • GLUT4 antibody
    • GTR4_HUMAN antibody
    • Insulin responsive glucose transporter type 4 antibody
    • kug antibody
    • SLC 2A4 antibody
    • SLC2A4 antibody
    • solute carrier family 2 (facilitated glucose transporter) member 4 antibody
    • Solute carrier family 2 member 4 antibody
    • Solute carrier family 2, facilitated glucose transporter member 4 antibody
    see all


  • 3T3-L1 lysate probed with Mouse anti Rat GLUT4 (ab35826) followed by Goat anti Mouse IgG:Dylight®800. Imaged using the LiCor Odyssey ®


This product has been referenced in:
  • Aimé P  et al. Modulation of olfactory sensitivity and glucose-sensing by the feeding state in obese Zucker rats. Front Behav Neurosci 8:326 (2014). IHC-Fr ; Rat . Read more (PubMed: 25278856) »
  • Reed SE  et al. A role for Rab14 in the endocytic trafficking of GLUT4 in 3T3-L1 adipocytes. J Cell Sci 126:1931-41 (2013). Mouse . Read more (PubMed: 23444368) »

See all 6 Publications for this product

Customer reviews and Q&As

Thank you for your enquiry regarding ab35826. I can confirm that ab35826 has been tested for Western blot application under reducing and denaturing conditions and the samples were boiled for 5 mins This antibody has not been characterized under n...

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