Product nameAnti-Glucose Transporter GLUT4 antibody [1F8]
See all Glucose Transporter GLUT4 primary antibodies
DescriptionMouse monoclonal [1F8] to Glucose Transporter GLUT4
SpecificityThis antibody is specific for the fat/muscle specific isoform of the Glucose Transporter GLUT4. We have data to indicate that this antibody may not cross react with Dog. However, this has not been conclusively tested and expression levels may vary in certain cell lines/tissues.
Tested applicationsSuitable for: ICC/IF, IP, IHC-Fr, WBmore details
Species reactivityReacts with: Mouse, Rat, Rabbit, Human, Monkey
Rat Glucose Transporter GLUT4 from partially purified vesicles.
EpitopeThis antibody has been shown to recognise an epitope in the cytoplasmic portion of the molecule.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium Azide
Constituents: PBS, pH 7.2
Concentration information loading...
PurityProtein G purified
- Pathways and Processes
- Metabolic signaling pathways
- Energy transfer pathways
- Energy Metabolism
Our Abpromise guarantee covers the use of ab35826 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 2.5 - 5 µg/ml.|
|IP||Use at an assay dependent concentration. Membrane proteins at a concentration of 1-2 mg/ml can be immunoadsorbed using this antibody coupled with agarose beads.|
|IHC-Fr||Use at an assay dependent concentration. PubMed: 20418694|
|WB||Use at an assay dependent concentration. Predicted molecular weight: 56 kDa. 5 µg/ml of antibody will detect Glucose Transporter protein contained in 50-200 µg of solubilised membrane.|
FunctionInsulin-regulated facilitative glucose transporter.
Tissue specificitySkeletal and cardiac muscles; brown and white fat.
Involvement in diseaseDiabetes mellitus, non-insulin-dependent
Sequence similaritiesBelongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family. Glucose transporter subfamily.
Cellular localizationCell membrane. Endomembrane system. Cytoplasm, perinuclear region. Localizes primarily to the perinuclear region, undergoing continued recycling to the plasma membrane where it is rapidly reinternalized. The dileucine internalization motif is critical for intracellular sequestration.
- Information by UniProt
- insulin-responsive antibody
- Glucose transporter GLUT 4 antibody
- Glucose transporter type 4 antibody
This product has been referenced in:
- Aimé P et al. Modulation of olfactory sensitivity and glucose-sensing by the feeding state in obese Zucker rats. Front Behav Neurosci 8:326 (2014). IHC-Fr ; Rat . Read more (PubMed: 25278856) »
- Reed SE et al. A role for Rab14 in the endocytic trafficking of GLUT4 in 3T3-L1 adipocytes. J Cell Sci 126:1931-41 (2013). Mouse . Read more (PubMed: 23444368) »