Anti-Glucose Transporter GLUT4 antibody (ab33780)
Key features and details
- Rabbit polyclonal to Glucose Transporter GLUT4
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Human, Recombinant fragment
- Isotype: IgG
Overview
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Product name
Anti-Glucose Transporter GLUT4 antibody
See all Glucose Transporter GLUT4 primary antibodies -
Description
Rabbit polyclonal to Glucose Transporter GLUT4 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human, Recombinant fragment
Predicted to work with: Rat, Sheep, Rabbit, Goat, Horse, Cow -
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 450 to the C-terminus of Human Glucose Transporter GLUT4.
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Positive control
- ICC: ioSkeletal Myocytes - Human iPSC-Derived Skeletal Myocytes and HeLa cells. WB: Partial tagged recombinant protein to GLUT4; Human heart and skeletal muscle tissue lysates. IHC-P: Human and mouse heart tissue; Rat skeletal muscle tissues. ICC: HeLa cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab33780 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (5) |
Use a concentration of 1 µg/ml. Predicted molecular weight: 55 kDa.
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IHC-P | (3) |
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ICC/IF |
Use a concentration of 1 - 5 µg/ml.
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Notes |
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WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 55 kDa. |
IHC-P
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
Use a concentration of 1 - 5 µg/ml. |
Target
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Function
Insulin-regulated facilitative glucose transporter. -
Tissue specificity
Skeletal and cardiac muscles; brown and white fat. -
Involvement in disease
Diabetes mellitus, non-insulin-dependent -
Sequence similarities
Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family. Glucose transporter subfamily. -
Post-translational
modificationsSumoylated. -
Cellular localization
Cell membrane. Endomembrane system. Cytoplasm, perinuclear region. Localizes primarily to the perinuclear region, undergoing continued recycling to the plasma membrane where it is rapidly reinternalized. The dileucine internalization motif is critical for intracellular sequestration. - Information by UniProt
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Database links
- Entrez Gene: 282359 Cow
- Entrez Gene: 6517 Human
- Entrez Gene: 20528 Mouse
- Entrez Gene: 25139 Rat
- Omim: 138190 Human
- SwissProt: Q27994 Cow
- SwissProt: P14672 Human
- SwissProt: P14142 Mouse
see all -
Alternative names
- insulin-responsive antibody
- Glucose transporter GLUT 4 antibody
- Glucose transporter type 4 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucose Transporter GLUT4 antibody (ab33780)
IHC image of Glucose Transporter GLUT4 staining in a section of formalin-fixed paraffin-embedded normal mouse heart performed on a Leica BOND™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab33780, 1ug/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucose Transporter GLUT4 antibody (ab33780)
IHC image of Glucose Transporter GLUT4 staining in a section of formalin-fixed paraffin-embedded normal human heart* performed on a Leica BOND™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab33780, 1ug/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
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ab33780 staining Glucose Transporter GLUT4 in HeLa cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab33780 at 1µg/ml and ab7291, Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control. Cells were then incubated with ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594), pre-adsorbed at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).
Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.
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Anti-Glucose Transporter GLUT4 antibody (ab33780) at 1 µg/ml + Partial tagged recombinant protein to GLUT4 at 0.1 µg
Secondary
IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Predicted band size: 55 kDa
Observed band size: 30 kDa why is the actual band size different from the predicted?ab33780 gave a positive signal against the partial recombinant GLUT4 protein which has an expected molecular weight of 30 kDa.
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Immunofluorescence staining of GLUT4 using ab33780 in ioSkeletal Myocytes - Human iPSC-Derived Skeletal Myocytes (ab277612), which were differentiated for 3 (left panel), 5 (middle panel) and 10 days (right panel) post induction.
The cells were fixed with 100% MeOH (5 min) and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab33780 at 5 µg/mL and ab7291, Mouse monoclonal [DM1A] to alpha Tubulin, at 1/1000 dilution. Cells were then incubated with ab150081, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (shown in green) and ab150120, Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed at 1/1000 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).
Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown. Gamma is adjusted to 1.5 in all channels.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucose Transporter GLUT4 antibody (ab33780)Image is courtesy of an Anonymous AbReview.
Immunohistochemical of PFA-fixed paraffin-embedded rat skeletal muscle skeletal tissue, labelling glucose transporter GLUT4 with ab33780 at a dilution of 1/200 incubated for 13 hours at 4°C in 1% BSA in TBS. Antigen retrival was with Tris-EDTA at pH 9.0 (heat mediated). Blocking was with 3% BSA incubated for 1 hour at 37°C. Secondary was a Goat anti-rabbit polyclonal Akaline Phosphotase conjugate at 1/200.
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All lanes : Anti-Glucose Transporter GLUT4 antibody (ab33780) at 1 µg/ml
Lane 1 : Recombinant Protein GLUT4 (Partial, Tagged) at 0.1 µg
Lane 2 : Heart (Human) Tissue Lysate at 20 µg
Lane 3 : Skeletal Muscle (Human) Tissue Lysate at 20 µg
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 55 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?
Exposure time: 4 minutes -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucose Transporter GLUT4 antibody (ab33780)
IHC image of Glucose Transporter GLUT4 staining in Mouse normal skeletal muscle formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab33780, 5µg/ml, for 15 mins at room temperature. A Goat anti-Rabbit biotinylated secondary antibody was used to detect the primary, and visualized using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (30)
ab33780 has been referenced in 30 publications.
- Jurek S et al. Optimizing adipogenic transdifferentiation of bovine mesenchymal stem cells: a prominent role of ascorbic acid in FABP4 induction. Adipocyte 9:35-50 (2020). PubMed: 31996081
- Zhou J et al. Xiaokeyinshui extract combination, a berberine-containing agent, exerts anti-diabetic and renal protective effects on rats in multi-target mechanisms. J Ethnopharmacol 262:113098 (2020). PubMed: 32726678
- Farrash W et al. Impacts of rat hindlimb Fndc5/irisin overexpression on muscle and adipose tissue metabolism. Am J Physiol Endocrinol Metab 318:E943-E955 (2020). PubMed: 32369414
- Furuhata R et al. Progesterone receptor membrane associated component 1 enhances obesity progression in mice by facilitating lipid accumulation in adipocytes. Commun Biol 3:479 (2020). PubMed: 32887925
- Kuo K et al. Maternal High-Fat Diet Consumption and Chronic Hyperandrogenemia Are Associated With Placental Dysfunction in Female Rhesus Macaques. Endocrinology 160:1937-1949 (2019). PubMed: 31180495