Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-Glucose Transporter GLUT4 antibody

Average
Abreviews
Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Mouse Tissue sections (Brain sections)
Antigen retrieval step
None
Permeabilization
No
Specification
Brain sections
Fixative
Paraformaldehyde

Other product details

Incubation time
18 hour(s) and 0 minute(s) · Temperature: 20°C · Diluent: PBS + 0.3 % Triton X100
Dilution
1/300

Secondary antibody

Dilution
1/1000
Name
Non-Abcam antibody was used: Goat anti-rabbit Alexa Fluor 488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor­ 488

Additional data

Additional Notes
This antibody produced some staining in many brain areas. The tissues were fixed (animals perfused fixed) with 4% PFA and later postfixed overnight in the same fixative. They were cryoprotected in 30% sucrose and cut using a cryostat.

Dr. Sophie Pezet

Verified customer

Submitted Aug 10 2009

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