Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-Glucose Transporter GLUT4 antibody

Inconclusive
Abreviews
Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Rat Tissue sections (Brain sections)
Specification
Brain sections
Fixative
Paraformaldehyde
Antigen retrieval step
None
Permeabilization
No

Other product details

Dilution
1/100
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 20°C · Diluent: PBS + 0.3 % Triton X100

Secondary antibody

Name
Non-Abcam antibody was used: Goat anti-rabbit Alexa Fluor 488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488

Additional data

Additional Notes
I used this antibody on sections of rat brain (30 um) in free floating. I tested the antibody at concentrations from 1:100 to 1:3000 and did not obtain any staining with all the dilutions tested. The positive control I used (antibody against NPY) gave a very nice staining on sister sections. Therefore, I think that this antibody does not work on PFA fixed, cryostat cut sections.

The tissues were fixed (animals perfused fixed) with 4% PFA and later postfixed overnight in the same fixative. They were cryoprotected in 30% sucrose and cut using a cryostat.
Abcam response
Many thanks for your feedback. This antibody is predicted to react with rat due to sequence homology although it has not yet been tested for its reactivity to rat GLUT4. We look forward to more feedback from our customers about how useful this antibody is in detecting rat protein in IHC.

Dr. Sophie Pezet

Verified customer

Submitted Jul 22 2009

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