Western blot abreview for Anti-Glucose Transporter GLUT4 antibody

Good
Abreviews
Application
Western blot
Sample
Mouse Tissue lysate - whole (MUSCLE, PROSTATE)
Loading amount
20 µg
Specification
MUSCLE, PROSTATE
Gel Running Conditions
Reduced Denaturing
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

Other product details

Dilution
1/500
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C

Secondary antibody

Name
Non-Abcam antibody was used: ANTI-RAbbIT
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/2000

Detection

Detection method
ECL
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: 41 kDa Non-specific: 120 kDa

Additional data

Additional Notes
Whole tissue lysate from mouse. Lane 1, muscle Lane 2, Prostate
Left panel, ab33780 Right panel, ab33780 with immunogenic peptide ab34088

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Submitted Feb 04 2010

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