Western blot abreview for Anti-Glucose Transporter GLUT4 antibody

Average
Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (Human Cardiomyocytes)
Loading amount
20 µg
Specification
Human Cardiomyocytes
Gel Running Conditions
Reduced Denaturing (8% SDS-PAGE gel with a 5% stacking gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C

Other product details

Dilution
1/1000
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% milk in TBST

Secondary antibody

Name
Non-Abcam antibody was used: anti-rabbit HRP conjugation HRP
Host species: Donkey
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/2500

Detection

Detection method
Pierce pico ecl kit
Exposure
30 second(s)
Bands
Specific: 55 kDa Non-specific: 30 kDa
Positive control
band seen in human, and rat cardiac tissue at about the correct size (55kDa), but abcam say they have seen a band at just over 30kDa, and there is a band of that size in the HC92 cell line sample.

Prof. Paul Townsend

Verified customer

Submitted Mar 24 2010

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