Flow Cytometry abreview for Anti-Glucose Transporter GLUT4 antibody

Inconclusive
Abreviews
Application
Flow Cytometry
Sample
Mouse Cell (A20 cell line, Mouse B cell lymphoma)
Specification
A20 cell line, Mouse B cell lymphoma
Preparation
Cell harvesting/tissue preparation method: suspension cells, so used directly.
Sample buffer: RPMI 1640 medium with 2 mM L-glutamine
Fixation
unfixed
Permeabilization
No
Gating Strategy
total stained cells

Other product details

Concentration
1 µg/ml
Incubation time
30 minute(s) · Temperature: 4°C · Diluent: FACS buffer (PBS with 0.1% azide)

Secondary antibody

Name
Non-Abcam antibody was used: anti-rabbit FITC
Host species: Donkey
Clonality: Polyclonal
Conjugation: Fluorescein
Concentration
1 µg/ml

Additional data

Additional Notes
No binding seen with FACS
Abcam response
Thank you for your honest and valuable feedback. Abcam has not tested ab33780 in flow cytometry and your data suggest it may not be suitable for this application under the conditions in which you tested. To confirm this finding, have you considered including a positive control like mouse skeletal muscle or cardiac muscle as the cells in these tissues have been shown to express GLUT4? We hope that your review will be helpful for future customers.

Prof. Paul Townsend

Verified customer

Submitted May 07 2010

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