Western blot abreview for Anti-Glucose Transporter GLUT4 antibody

Poor
Abreviews
Application
Western blot
Sample
Mouse Cell lysate - whole cell (mouse Cell lysate - whole cell (mouse Cardiomyocyt)
Loading amount
20 µg
Specification
mouse Cell lysate - whole cell (mouse Cardiomyocyt
Gel Running Conditions
Reduced Denaturing (8% SDS-PAGE gel with a 5% stacking gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C

Other product details

Dilution
1/1000
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% milk in TBST

Secondary antibody

Name
Non-Abcam antibody was used: anti-rabbit HRP
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/2500

Detection

Detection method
Pierce pico ecl kit
Exposure
30 second(s)
Bands
Specific: 55 kDa Non-specific: 30 kDa
Positive control
band seen in human, and rat cardiac tissue at about the correct size (55kDa), faint (?) band seen in the mouse sample - wouldn't be confident about it.
Abcam says they have seen a band at just over 30kDa, and there is a band of that size in the HC92 cell line sample.

Additional data

Abcam response
Thank you for your honest and valuable feedback. We are sorry this antibody did not work under the experimental conditions in which you tested. We hope that your review will be useful to future customers.

Prof. Paul Townsend

Verified customer

Submitted May 04 2010

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