Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Glucose Transporter GLUT4 antibody

Excellent
Abreviews
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (Skeletal muscle)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris-EDTA pH 9.0
Permeabilization
No
Specification
Skeletal muscle
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 37°C
Fixative
Paraformaldehyde

Other product details

Incubation time
13 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 1% BSA in TBS
Dilution
1/200

Secondary antibody

Dilution
1/200
Name
Non-Abcam antibody was used: Goat anti-rabbit IgG F(ab')2-AP (sc-3838)
Host species: Goat
Clonality: Polyclonal
Conjugation: Alkaline Phosphatase

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Submitted Sep 11 2015

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