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    glucose-transporter-glut4-antibody-mabcam48547-ab48547.pdf

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Anti-Glucose Transporter GLUT4 antibody [mAbcam48547] (ab48547)

  • Datasheet
Reviews (3) Submit a question References (6)

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Western blot - Anti-Glucose Transporter GLUT4 antibody [mAbcam48547] (ab48547)
  • Flow Cytometry - Anti-Glucose Transporter GLUT4 antibody [mAbcam48547] (ab48547)

Key features and details

  • Mouse monoclonal [mAbcam48547] to Glucose Transporter GLUT4
  • Suitable for: WB, Flow Cyt
  • Reacts with: Mouse, Recombinant fragment
  • Isotype: IgG2b

You may also be interested in

Primary
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Anti-Glucose Transporter GLUT2 antibody [EPR16550] (ab192599)
Secondary
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Goat Anti-Mouse IgG H&L (HRP) (ab205719)
Conjugation
Product image
Alexa Fluor® 488 Conjugation Kit (Fast) - Lightning-Link® (ab236553)

View more associated products

Overview

  • Product name

    Anti-Glucose Transporter GLUT4 antibody [mAbcam48547]
    See all Glucose Transporter GLUT4 primary antibodies
  • Description

    Mouse monoclonal [mAbcam48547] to Glucose Transporter GLUT4
  • Host species

    Mouse
  • Tested applications

    Suitable for: WB, Flow Cytmore details
  • Species reactivity

    Reacts with: Mouse, Recombinant fragment
    Predicted to work with: Rat, Sheep, Rabbit, Goat, Horse, Cow
  • Immunogen

    Synthetic peptide corresponding to Human Glucose Transporter GLUT4 aa 450 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab34088, ab34089)

  • Positive control

    • WB: ab48547 gave a positive signal against the partial recombinant GLUT4 protein which has an expected molecular weight of 30 kDa. Flow cyt: NSO cells.
  • General notes

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity

    Protein G purified
  • Clonality

    Monoclonal
  • Clone number

    mAbcam48547
  • Myeloma

    Sp2/0
  • Isotype

    IgG2b
  • Light chain type

    kappa
  • Research areas

    • Signal Transduction
    • Metabolism
    • Energy Metabolism
    • Stem Cells
    • Mesenchymal Stem Cells
    • Adipogenesis
    • Cardiovascular
    • Heart
    • Cardiac metabolism
    • Cancer
    • Cancer Metabolism
    • Metabolic signaling pathway
    • Metabolism of carbohydrates
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Carbohydrate metabolism
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Energy transfer pathways
    • Energy Metabolism
    • Metabolism
    • Types of disease
    • Diabetes
    • Metabolism
    • Types of disease
    • Cancer

Associated products

  • Alternative Versions

    • Anti-Glucose Transporter GLUT4 antibody [mAbcam48547] - BSA and Azide free (ab238661)
  • ChIP Related Products

    • Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed (ab96879)
  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
  • Conjugation kits

    • PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
    • APC Conjugation Kit - Lightning-Link® (ab201807)
  • Immunizing Peptide (Blocking)

    • Human Glucose Transporter GLUT4 peptide (ab34088)
  • Isotype control

    • Mouse IgG2b, kappa monoclonal [7E10G10] - Isotype Control (ab170192)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab48547 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 30 kDa (predicted molecular weight: 54 kDa).
Flow Cyt
Use 1µg for 106 cells.

ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.

Notes
WB
Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 30 kDa (predicted molecular weight: 54 kDa).
Flow Cyt
Use 1µg for 106 cells.

ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.

Target

  • Function

    Insulin-regulated facilitative glucose transporter.
  • Tissue specificity

    Skeletal and cardiac muscles; brown and white fat.
  • Involvement in disease

    Diabetes mellitus, non-insulin-dependent
  • Sequence similarities

    Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family. Glucose transporter subfamily.
  • Post-translational
    modifications

    Sumoylated.
  • Cellular localization

    Cell membrane. Endomembrane system. Cytoplasm, perinuclear region. Localizes primarily to the perinuclear region, undergoing continued recycling to the plasma membrane where it is rapidly reinternalized. The dileucine internalization motif is critical for intracellular sequestration.
  • Target information above from: UniProt accession P14672 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 282359 Cow
    • Entrez Gene: 20528 Mouse
    • Entrez Gene: 25139 Rat
    • SwissProt: Q27994 Cow
    • SwissProt: P14142 Mouse
    • SwissProt: P19357 Rat
    • Unigene: 10661 Mouse
    • Unigene: 1314 Rat
    • Alternative names

      • insulin-responsive antibody
      • Glucose transporter GLUT 4 antibody
      • Glucose transporter type 4 antibody
      • Glucose transporter type 4 insulin responsive antibody
      • GLUT 4 antibody
      • GLUT-4 antibody
      • GLUT4 antibody
      • GTR4_HUMAN antibody
      • Insulin responsive glucose transporter type 4 antibody
      • kug antibody
      • SLC 2A4 antibody
      • SLC2A4 antibody
      • solute carrier family 2 (facilitated glucose transporter) member 4 antibody
      • Solute carrier family 2 member 4 antibody
      • Solute carrier family 2, facilitated glucose transporter member 4 antibody
      see all

    Images

    • Western blot - Anti-Glucose Transporter GLUT4 antibody [mAbcam48547] (ab48547)
      Western blot - Anti-Glucose Transporter GLUT4 antibody [mAbcam48547] (ab48547)
      Anti-Glucose Transporter GLUT4 antibody [mAbcam48547] (ab48547) at 5 µg/ml + Partial tagged recombinant protein to GLUT4 at 0.1 µg

      Secondary
      Rabbit polyclonal to Mouse IgG - H&L (HRP) at 1/3000 dilution

      Performed under reducing conditions.

      Predicted band size: 54 kDa
      Observed band size: 30 kDa why is the actual band size different from the predicted?



      ab48547 gave a positive signal against the partial recombinant GLUT4 protein which has an expected molecular weight of 30 kDa.
    • Flow Cytometry - Anti-Glucose Transporter GLUT4 antibody [mAbcam48547] (ab48547)
      Flow Cytometry - Anti-Glucose Transporter GLUT4 antibody [mAbcam48547] (ab48547)

      Overlay histogram showing NSO cells stained with ab48547 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab48547, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879 at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in NSO cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

    Protocols

    • Flow cytometry protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet download

      Download

    References (6)

    Publishing research using ab48547? Please let us know so that we can cite the reference in this datasheet.

    ab48547 has been referenced in 6 publications.

    • Panahi M  et al. Insulin-Independent and Dependent Glucose Transporters in Brain Mural Cells in CADASIL. Front Genet 11:1022 (2020). PubMed: 33101365
    • Ono E  et al. Sweat glucose and GLUT2 expression in atopic dermatitis: Implication for clinical manifestation and treatment. PLoS One 13:e0195960 (2018). PubMed: 29677207
    • Li J  et al. Inhibition of insulin/PI3K/AKT signaling decreases adipose Sortilin 1 in mice and 3T3-L1 adipocytes. Biochim Biophys Acta Mol Basis Dis 1863:2924-2933 (2017). PubMed: 28844948
    • Mano Y  et al. Correlation between biological marker expression and fluorine-18 fluorodeoxyglucose uptake in hepatocellular carcinoma. Am J Clin Pathol 142:391-7 (2014). Human . PubMed: 25125631
    • Karim S  et al. Dysregulated hepatic expression of glucose transporters in chronic disease: contribution of semicarbazide-sensitive amine oxidase to hepatic glucose uptake. Am J Physiol Gastrointest Liver Physiol 307:G1180-90 (2014). PubMed: 25342050
    • Mattmiller SA  et al. Glucose transporter and hypoxia-associated gene expression in the mammary gland of transition dairy cattle. J Dairy Sci 94:2912-22 (2011). PubMed: 21605761

    Customer reviews and Q&As

    Show All Reviews Q&A
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    1-3 of 3 Abreviews or Q&A

    Immunocytochemistry abreview for Anti-Glucose Transporter GLUT4 antibody [mAbcam48547]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry
    Sample
    Human Cultured Cells (SH-SY5Y cells)
    Permeabilization
    Yes - 0.1% Triton-X100 in PBS 10'
    Specification
    SH-SY5Y cells
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2.5% · Temperature: 20°C
    Fixative
    Formaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Apr 11 2022

    Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-Glucose Transporter GLUT4 antibody [mAbcam48547]

    Below Average
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (PFA perfusion fixed frozen sections)
    Sample
    Rat Tissue sections (Brain sections)
    Specification
    Brain sections
    Fixative
    Paraformaldehyde
    Antigen retrieval step
    None
    Permeabilization
    No
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    DR. Sophie Pezet

    Verified customer

    Submitted Jul 27 2009

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Glucose Transporter GLUT4 antibody [mAbcam48547]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (heart)
    Specification
    heart
    Fixative
    Formaldehyde
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citrate Buffer pH6.0
    Permeabilization
    No
    Blocking step
    5 minutes of peroxidase block then 10 minutes of protein block. These are ready-to-use reagents purchased from Dako as blocking agent for 15 minute(s) · Concentration: 100% · Temperature: 20°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Mr. Antibody Solutions

    Verified customer

    Submitted Jan 22 2009

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