Anti-Glucose Transporter GLUT4 antibody [mAbcam48547] (ab48547)
![Western blot - Anti-Glucose Transporter GLUT4 antibody [mAbcam48547] (ab48547)](https://www.abcam.com/ps/products/48/ab48547/Images/ab48547-61041-anti-glucose-transporter-glut4-antibody-mabcam48547-western-blot.jpg "Western blot - Anti-Glucose Transporter GLUT4 antibody [mAbcam48547] (ab48547)")
Key features and details
- Mouse monoclonal [mAbcam48547] to Glucose Transporter GLUT4
- Suitable for: WB, Flow Cyt
- Reacts with: Mouse, Recombinant fragment
- Isotype: IgG2b
Overview
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Product name
Anti-Glucose Transporter GLUT4 antibody [mAbcam48547]
See all Glucose Transporter GLUT4 primary antibodies -
Description
Mouse monoclonal [mAbcam48547] to Glucose Transporter GLUT4 -
Host species
Mouse -
Tested applications
Suitable for: WB, Flow Cytmore details -
Species reactivity
Reacts with: Mouse, Recombinant fragment
Predicted to work with: Rat, Sheep, Rabbit, Goat, Horse, Cow
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Immunogen
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Positive control
- WB: ab48547 gave a positive signal against the partial recombinant GLUT4 protein which has an expected molecular weight of 30 kDa. Flow cyt: NSO cells.
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General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
mAbcam48547 -
Myeloma
Sp2/0 -
Isotype
IgG2b -
Light chain type
kappa -
Research areas
Associated products
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Alternative Versions
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ChIP Related Products
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Compatible Secondaries
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Conjugation kits
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Immunizing Peptide (Blocking)
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab48547 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 30 kDa (predicted molecular weight: 54 kDa).
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| Flow Cyt |
Use 1µg for 106 cells.
ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
| Notes |
|---|
|
WB
Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 30 kDa (predicted molecular weight: 54 kDa). |
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Flow Cyt
Use 1µg for 106 cells. ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
Target
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Function
Insulin-regulated facilitative glucose transporter. -
Tissue specificity
Skeletal and cardiac muscles; brown and white fat. -
Involvement in disease
Diabetes mellitus, non-insulin-dependent -
Sequence similarities
Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family. Glucose transporter subfamily. -
Post-translational
modificationsSumoylated. -
Cellular localization
Cell membrane. Endomembrane system. Cytoplasm, perinuclear region. Localizes primarily to the perinuclear region, undergoing continued recycling to the plasma membrane where it is rapidly reinternalized. The dileucine internalization motif is critical for intracellular sequestration. - Information by UniProt
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Database links
- Entrez Gene: 282359 Cow
- Entrez Gene: 20528 Mouse
- Entrez Gene: 25139 Rat
- SwissProt: Q27994 Cow
- SwissProt: P14142 Mouse
- SwissProt: P19357 Rat
- Unigene: 10661 Mouse
- Unigene: 1314 Rat
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Alternative names
- insulin-responsive antibody
- Glucose transporter GLUT 4 antibody
- Glucose transporter type 4 antibody
see all
Images
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Western blot - Anti-Glucose Transporter GLUT4 antibody [mAbcam48547] (ab48547)Anti-Glucose Transporter GLUT4 antibody [mAbcam48547] (ab48547) at 5 µg/ml + Partial tagged recombinant protein to GLUT4 at 0.1 µg
Secondary
Rabbit polyclonal to Mouse IgG - H&L (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 54 kDa
Observed band size: 30 kDa why is the actual band size different from the predicted?
ab48547 gave a positive signal against the partial recombinant GLUT4 protein which has an expected molecular weight of 30 kDa. -
![Flow Cytometry - Anti-Glucose Transporter GLUT4 antibody [mAbcam48547] (ab48547)](https://www.abcam.com/ps/products/48/ab48547/Images/ab48547-61039-anti-glucose-transporter-glut4-antibody-mabcam48547-flow-cytometry.jpg)
Flow Cytometry - Anti-Glucose Transporter GLUT4 antibody [mAbcam48547] (ab48547)Overlay histogram showing NSO cells stained with ab48547 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab48547, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879 at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in NSO cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
Datasheets and documents
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Datasheet download
References (6)
ab48547 has been referenced in 6 publications.
- Panahi M et al. Insulin-Independent and Dependent Glucose Transporters in Brain Mural Cells in CADASIL. Front Genet 11:1022 (2020). PubMed: 33101365
- Ono E et al. Sweat glucose and GLUT2 expression in atopic dermatitis: Implication for clinical manifestation and treatment. PLoS One 13:e0195960 (2018). PubMed: 29677207
- Li J et al. Inhibition of insulin/PI3K/AKT signaling decreases adipose Sortilin 1 in mice and 3T3-L1 adipocytes. Biochim Biophys Acta Mol Basis Dis 1863:2924-2933 (2017). PubMed: 28844948
- Mano Y et al. Correlation between biological marker expression and fluorine-18 fluorodeoxyglucose uptake in hepatocellular carcinoma. Am J Clin Pathol 142:391-7 (2014). Human . PubMed: 25125631
- Karim S et al. Dysregulated hepatic expression of glucose transporters in chronic disease: contribution of semicarbazide-sensitive amine oxidase to hepatic glucose uptake. Am J Physiol Gastrointest Liver Physiol 307:G1180-90 (2014). PubMed: 25342050
- Mattmiller SA et al. Glucose transporter and hypoxia-associated gene expression in the mammary gland of transition dairy cattle. J Dairy Sci 94:2912-22 (2011). PubMed: 21605761
