Anti-Glucose Transporter GLUT4 antibody [mAbcam48547] - BSA and Azide free (ab238661)

Overview

  • Product name

    Anti-Glucose Transporter GLUT4 antibody [mAbcam48547] - BSA and Azide free
    See all Glucose Transporter GLUT4 primary antibodies
  • Description

    Mouse monoclonal [mAbcam48547] to Glucose Transporter GLUT4 - BSA and Azide free
  • Host species

    Mouse
  • Tested applications

    Suitable for: WB, IHC-P, Flow Cytmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Sheep, Rabbit, Goat, Horse, Cow
  • Immunogen

    Synthetic peptide within Human Glucose Transporter GLUT4 aa 450 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
    (Peptide available as ab34088, ab34089)

  • Positive control

    • WB: ab48547 gave a positive signal against the partial recombinant GLUT4 protein which has an expected molecular weight of 30 kDa. IHC-P: Human heart tissue. Flow cyt: NSO cells.
  • General notes

    ab238661 is a PBS-only buffer format of ab48547.

    This antibody clone is manufactured by Abcam.

    If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.

Properties

Applications

Our Abpromise guarantee covers the use of ab238661 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Detects a band of approximately 30 kDa (predicted molecular weight: 54 kDa).Can be blocked with Human Glucose Transporter GLUT4 peptide (ab34088).
IHC-P Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration.

ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.

Target

  • Function

    Insulin-regulated facilitative glucose transporter.
  • Tissue specificity

    Skeletal and cardiac muscles; brown and white fat.
  • Involvement in disease

    Diabetes mellitus, non-insulin-dependent
  • Sequence similarities

    Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family. Glucose transporter subfamily.
  • Post-translational
    modifications

    Sumoylated.
  • Cellular localization

    Cell membrane. Endomembrane system. Cytoplasm, perinuclear region. Localizes primarily to the perinuclear region, undergoing continued recycling to the plasma membrane where it is rapidly reinternalized. The dileucine internalization motif is critical for intracellular sequestration.
  • Information by UniProt
  • Database links

  • Alternative names

    • insulin-responsive antibody
    • Glucose transporter GLUT 4 antibody
    • Glucose transporter type 4 antibody
    • Glucose transporter type 4 insulin responsive antibody
    • GLUT 4 antibody
    • GLUT-4 antibody
    • GLUT4 antibody
    • GTR4_HUMAN antibody
    • Insulin responsive glucose transporter type 4 antibody
    • kug antibody
    • SLC 2A4 antibody
    • SLC2A4 antibody
    • solute carrier family 2 (facilitated glucose transporter) member 4 antibody
    • Solute carrier family 2 member 4 antibody
    • Solute carrier family 2, facilitated glucose transporter member 4 antibody
    see all

Images

  • Overlay histogram showing NSO cells stained with ab48547 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab48547, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879 at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in NSO cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

    This image was produced using the same antibody clone but in a different formulation; PBS and sodium azide (ab48547).

References

ab238661 has not yet been referenced specifically in any publications.

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