Recombinant Anti-Glucose Transporter GLUT4 (phospho S488) antibody [EPR930(2)] (ab188317)
![Western blot - Anti-Glucose Transporter GLUT4 (phospho S488) antibody [EPR930(2)] (ab188317)](https://www.abcam.com/ps/products/188/ab188317/Images/ab188317-468496-anti-glucose-transporter-glut4-antibody-western-blot-heart-human-mouse.jpg "Western blot - Anti-Glucose Transporter GLUT4 (phospho S488) antibody [EPR930(2)] (ab188317)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR930(2)] to Glucose Transporter GLUT4 (phospho S488)
- Suitable for: WB, Dot blot
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-Glucose Transporter GLUT4 (phospho S488) antibody [EPR930(2)]
See all Glucose Transporter GLUT4 primary antibodies -
Description
Rabbit monoclonal [EPR930(2)] to Glucose Transporter GLUT4 (phospho S488) -
Host species
Rabbit -
Tested applications
Suitable for: WB, Dot blotmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Human fetal heart, mouse heart, rat heart
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR930(2) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab188317 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | (2) |
1/1000 - 1/10000. Detects a band of approximately 45 kDa (predicted molecular weight: 55 kDa).
We recommend not to boil the samples before loading to get desirable WB results. |
| Dot blot |
Use at an assay dependent concentration.
|
| Notes |
|---|
|
WB
1/1000 - 1/10000. Detects a band of approximately 45 kDa (predicted molecular weight: 55 kDa). We recommend not to boil the samples before loading to get desirable WB results. |
|
Dot blot
Use at an assay dependent concentration. |
Target
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Function
Insulin-regulated facilitative glucose transporter. -
Tissue specificity
Skeletal and cardiac muscles; brown and white fat. -
Involvement in disease
Diabetes mellitus, non-insulin-dependent -
Sequence similarities
Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family. Glucose transporter subfamily. -
Post-translational
modificationsSumoylated. -
Cellular localization
Cell membrane. Endomembrane system. Cytoplasm, perinuclear region. Localizes primarily to the perinuclear region, undergoing continued recycling to the plasma membrane where it is rapidly reinternalized. The dileucine internalization motif is critical for intracellular sequestration. - Information by UniProt
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Database links
- Entrez Gene: 6517 Human
- Entrez Gene: 20528 Mouse
- Entrez Gene: 25139 Rat
- Omim: 138190 Human
- SwissProt: P14672 Human
- SwissProt: P14142 Mouse
- SwissProt: P19357 Rat
- Unigene: 380691 Human
see all -
Alternative names
- insulin-responsive antibody
- Glucose transporter GLUT 4 antibody
- Glucose transporter type 4 antibody
see all
Images
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Western blot - Anti-Glucose Transporter GLUT4 (phospho S488) antibody [EPR930(2)] (ab188317)All lanes : Anti-Glucose Transporter GLUT4 (phospho S488) antibody [EPR930(2)] (ab188317) at 1/1000 dilution
Lane 1 : Human heart lysate, boiled
Lane 2 : Human heart lysate, unboiled
Lane 3 : Mouse heart lysate, boiled
Lane 4 : Mouse heart lysate, unboiled
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 55 kDa
Observed band size: 45 kDa why is the actual band size different from the predicted?Lanes 1 and 2: 7 seconds exposure
Lanes 3 and 4: 20 seconds exposure
Blocking and diluting buffer: 5% NFDM/TBST
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![Western blot - Anti-Glucose Transporter GLUT4 (phospho S488) antibody [EPR930(2)] (ab188317)](https://www.abcam.com/ps/products/188/ab188317/Images/ab188317-274088-anti-glucose-transporter-glut4-phospho-s488-antibody-epr9302-western-blot.jpg)
Western blot - Anti-Glucose Transporter GLUT4 (phospho S488) antibody [EPR930(2)] (ab188317)All lanes : Anti-Glucose Transporter GLUT4 (phospho S488) antibody [EPR930(2)] (ab188317) at 1/1000 dilution
Lane 1 : Human fetal heart whole cell lysates
Lane 2 : Human fetal heart whole cell lysates with Phosphatase
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, Peroxidase conjugated (DC03L) at 1/20000 dilution
Predicted band size: 55 kDa
Observed band size: 45 kDa why is the actual band size different from the predicted?
Exposure time: 1 secondBlocking/Diluting buffer and concentration 5% NFDM/TBST
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![Dot Blot - Anti-Glucose Transporter GLUT4 (phospho S488) antibody [EPR930(2)] (ab188317)](https://www.abcam.com/ps/products/188/ab188317/Images/ab188317-268165-anti-glucose-transporter-glut4-phospho-s488-antibody-epr9302-dot-blot.jpg)
Dot Blot - Anti-Glucose Transporter GLUT4 (phospho S488) antibody [EPR930(2)] (ab188317)Dot Blot analysis of Lane 1: Glucose Transporter GLUT4 (pS488) phospho peptide and Lane 2: Glucose Transporter GLUT4 non-phospho peptide labeling Glucose Transporter GLUT4 (phospho S488) with ab188317 at 1/1000 dilution.
5% NFDM/TBST was used as the diluting and blocking buffer. ab97051 Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated was used as the secondary antibody at 1/100000 dilution. Exposure time: 3 minutes.
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![Western blot - Anti-Glucose Transporter GLUT4 (phospho S488) antibody [EPR930(2)] (ab188317)](https://www.abcam.com/ps/products/188/ab188317/Images/ab188317-221837-anti-glucose-transporter-glut4-phospho-s488-antibody-epr9302-western-blot.jpg)
Western blot - Anti-Glucose Transporter GLUT4 (phospho S488) antibody [EPR930(2)] (ab188317)All lanes : Anti-Glucose Transporter GLUT4 (phospho S488) antibody [EPR930(2)] (ab188317) at 1/5000 dilution
Lane 1 : Mouse heart
Lane 2 : Rat heart
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 55 kDa
Observed band size: 45 kDa why is the actual band size different from the predicted? -
![Western blot - Anti-Glucose Transporter GLUT4 (phospho S488) antibody [EPR930(2)] (ab188317)](https://www.abcam.com/ps/products/188/ab188317/Images/ab188317-221836-anti-glucose-transporter-glut4-phospho-s488-antibody-epr9302-western-blot.jpg)
Western blot - Anti-Glucose Transporter GLUT4 (phospho S488) antibody [EPR930(2)] (ab188317)All lanes : Anti-Glucose Transporter GLUT4 (phospho S488) antibody [EPR930(2)] (ab188317) at 1/20000 dilution
Lane 1 : Untreated human fetal heart
Lane 2 : Lambda phosphorylase treated human fetal heart
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 55 kDa
Observed band size: 45 kDa why is the actual band size different from the predicted? -
![Anti-Glucose Transporter GLUT4 (phospho S488) antibody [EPR930(2)] (ab188317)](https://www.abcam.com/ps/products/188/ab188317/Images/ab188317-5-benefits-of-recombinant-antibodies.png)
Anti-Glucose Transporter GLUT4 (phospho S488) antibody [EPR930(2)] (ab188317)
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (5)
ab188317 has been referenced in 5 publications.
- Rahimi G et al. A combination of herbal compound (SPTC) along with exercise or metformin more efficiently alleviated diabetic complications through down-regulation of stress oxidative pathway upon activating Nrf2-Keap1 axis in AGE rich diet-induced type 2 diabetic mice. Nutr Metab (Lond) 18:14 (2021). PubMed: 33468193
- Montt-Guevara MM et al. D-Chiro-Inositol Regulates Insulin Signaling in Human Adipocytes. Front Endocrinol (Lausanne) 12:660815 (2021). PubMed: 33859622
- Zhuo J et al. Evaluation of type 2 diabetic mellitus animal models via interactions between insulin and mitogen-activated protein kinase signaling pathways induced by a high fat and sugar diet and streptozotocin. Mol Med Rep 17:5132-5142 (2018). PubMed: 29393432
- Wang N et al. Hydroxytyrosol ameliorates insulin resistance by modulating endoplasmic reticulum stress and prevents hepatic steatosis in diet-induced obesity mice. J Nutr Biochem 57:180-188 (2018). WB ; Mouse . PubMed: 29747118
- Liu Z et al. Adiponectin reduces ER stress-induced apoptosis through PPARa transcriptional regulation of ATF2 in mouse adipose. Cell Death Dis 7:e2487 (2016). WB ; Mouse . PubMed: 27882945
