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    glucose-transporter-glut4-phospho-s488-antibody-epr9302-ab188317.pdf

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RecombinantRabMAb

Recombinant Anti-Glucose Transporter GLUT4 (phospho S488) antibody [EPR930(2)] (ab188317)

  • Datasheet
  • Certificate of Compliance
Reviews (3) Submit a question References (5)

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Western blot - Anti-Glucose Transporter GLUT4 (phospho S488) antibody [EPR930(2)] (ab188317)
  • Western blot - Anti-Glucose Transporter GLUT4 (phospho S488) antibody [EPR930(2)] (ab188317)
  • Dot Blot - Anti-Glucose Transporter GLUT4 (phospho S488) antibody [EPR930(2)] (ab188317)
  • Western blot - Anti-Glucose Transporter GLUT4 (phospho S488) antibody [EPR930(2)] (ab188317)
  • Western blot - Anti-Glucose Transporter GLUT4 (phospho S488) antibody [EPR930(2)] (ab188317)
  • Anti-Glucose Transporter GLUT4 (phospho S488) antibody [EPR930(2)] (ab188317)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR930(2)] to Glucose Transporter GLUT4 (phospho S488)
  • Suitable for: WB, Dot blot
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free

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Overview

  • Product name

    Anti-Glucose Transporter GLUT4 (phospho S488) antibody [EPR930(2)]
    See all Glucose Transporter GLUT4 primary antibodies
  • Description

    Rabbit monoclonal [EPR930(2)] to Glucose Transporter GLUT4 (phospho S488)
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, Dot blotmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human fetal heart, mouse heart, rat heart
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR930(2)
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Metabolism
    • Energy Metabolism
    • Stem Cells
    • Mesenchymal Stem Cells
    • Adipogenesis
    • Cardiovascular
    • Heart
    • Cardiac metabolism
    • Cancer
    • Cancer Metabolism
    • Metabolic signaling pathway
    • Metabolism of carbohydrates
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Carbohydrate metabolism
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Energy transfer pathways
    • Energy Metabolism
    • Metabolism
    • Types of disease
    • Diabetes

Associated products

  • Alternative Versions

    • Anti-Glucose Transporter GLUT4 (phospho S488) antibody [EPR930(2)] - BSA and Azide free (ab250949)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • Mouse heart normal tissue lysate - total protein (ab30291)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab188317 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB (2)
1/1000 - 1/10000. Detects a band of approximately 45 kDa (predicted molecular weight: 55 kDa).

We recommend not to boil the samples before loading to get desirable WB results.

Dot blot
Use at an assay dependent concentration.
Notes
WB
1/1000 - 1/10000. Detects a band of approximately 45 kDa (predicted molecular weight: 55 kDa).

We recommend not to boil the samples before loading to get desirable WB results.

Dot blot
Use at an assay dependent concentration.

Target

  • Function

    Insulin-regulated facilitative glucose transporter.
  • Tissue specificity

    Skeletal and cardiac muscles; brown and white fat.
  • Involvement in disease

    Diabetes mellitus, non-insulin-dependent
  • Sequence similarities

    Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family. Glucose transporter subfamily.
  • Post-translational
    modifications

    Sumoylated.
  • Cellular localization

    Cell membrane. Endomembrane system. Cytoplasm, perinuclear region. Localizes primarily to the perinuclear region, undergoing continued recycling to the plasma membrane where it is rapidly reinternalized. The dileucine internalization motif is critical for intracellular sequestration.
  • Target information above from: UniProt accession P14672 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 6517 Human
    • Entrez Gene: 20528 Mouse
    • Entrez Gene: 25139 Rat
    • Omim: 138190 Human
    • SwissProt: P14672 Human
    • SwissProt: P14142 Mouse
    • SwissProt: P19357 Rat
    • Unigene: 380691 Human
    • Unigene: 10661 Mouse
    • Unigene: 1314 Rat
    see all
  • Alternative names

    • insulin-responsive antibody
    • Glucose transporter GLUT 4 antibody
    • Glucose transporter type 4 antibody
    • Glucose transporter type 4 insulin responsive antibody
    • GLUT 4 antibody
    • GLUT-4 antibody
    • GLUT4 antibody
    • GTR4_HUMAN antibody
    • Insulin responsive glucose transporter type 4 antibody
    • kug antibody
    • SLC 2A4 antibody
    • SLC2A4 antibody
    • solute carrier family 2 (facilitated glucose transporter) member 4 antibody
    • Solute carrier family 2 member 4 antibody
    • Solute carrier family 2, facilitated glucose transporter member 4 antibody
    see all

Images

  • Western blot - Anti-Glucose Transporter GLUT4 (phospho S488) antibody [EPR930(2)] (ab188317)
    Western blot - Anti-Glucose Transporter GLUT4 (phospho S488) antibody [EPR930(2)] (ab188317)
    All lanes : Anti-Glucose Transporter GLUT4 (phospho S488) antibody [EPR930(2)] (ab188317) at 1/1000 dilution

    Lane 1 : Human heart lysate, boiled
    Lane 2 : Human heart lysate, unboiled
    Lane 3 : Mouse heart lysate, boiled
    Lane 4 : Mouse heart lysate, unboiled

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Predicted band size: 55 kDa
    Observed band size: 45 kDa why is the actual band size different from the predicted?



    Lanes 1 and 2: 7 seconds exposure

    Lanes 3 and 4: 20 seconds exposure

    Blocking and diluting buffer: 5% NFDM/TBST

  • Western blot - Anti-Glucose Transporter GLUT4 (phospho S488) antibody [EPR930(2)] (ab188317)
    Western blot - Anti-Glucose Transporter GLUT4 (phospho S488) antibody [EPR930(2)] (ab188317)
    All lanes : Anti-Glucose Transporter GLUT4 (phospho S488) antibody [EPR930(2)] (ab188317) at 1/1000 dilution

    Lane 1 : Human fetal heart whole cell lysates
    Lane 2 : Human fetal heart whole cell lysates with Phosphatase

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, Peroxidase conjugated (DC03L) at 1/20000 dilution

    Predicted band size: 55 kDa
    Observed band size: 45 kDa why is the actual band size different from the predicted?


    Exposure time: 1 second


    Blocking/Diluting buffer and concentration 5% NFDM/TBST

  • Dot Blot - Anti-Glucose Transporter GLUT4 (phospho S488) antibody [EPR930(2)] (ab188317)
    Dot Blot - Anti-Glucose Transporter GLUT4 (phospho S488) antibody [EPR930(2)] (ab188317)

    Dot Blot analysis of Lane 1: Glucose Transporter GLUT4 (pS488) phospho peptide and Lane 2: Glucose Transporter GLUT4 non-phospho peptide labeling Glucose Transporter GLUT4 (phospho S488) with ab188317 at 1/1000 dilution.

    5% NFDM/TBST was used as the diluting and blocking buffer. ab97051 Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated was used as the secondary antibody at 1/100000 dilution. Exposure time: 3 minutes.

  • Western blot - Anti-Glucose Transporter GLUT4 (phospho S488) antibody [EPR930(2)] (ab188317)
    Western blot - Anti-Glucose Transporter GLUT4 (phospho S488) antibody [EPR930(2)] (ab188317)
    All lanes : Anti-Glucose Transporter GLUT4 (phospho S488) antibody [EPR930(2)] (ab188317) at 1/5000 dilution

    Lane 1 : Mouse heart
    Lane 2 : Rat heart

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 55 kDa
    Observed band size: 45 kDa why is the actual band size different from the predicted?

  • Western blot - Anti-Glucose Transporter GLUT4 (phospho S488) antibody [EPR930(2)] (ab188317)
    Western blot - Anti-Glucose Transporter GLUT4 (phospho S488) antibody [EPR930(2)] (ab188317)
    All lanes : Anti-Glucose Transporter GLUT4 (phospho S488) antibody [EPR930(2)] (ab188317) at 1/20000 dilution

    Lane 1 : Untreated human fetal heart
    Lane 2 : Lambda phosphorylase treated human fetal heart

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 55 kDa
    Observed band size: 45 kDa why is the actual band size different from the predicted?

  • Anti-Glucose Transporter GLUT4 (phospho S488) antibody [EPR930(2)] (ab188317)
    Anti-Glucose Transporter GLUT4 (phospho S488) antibody [EPR930(2)] (ab188317)

Protocols

  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

  • Datasheet download

    Download

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

References (5)

Publishing research using ab188317? Please let us know so that we can cite the reference in this datasheet.

ab188317 has been referenced in 5 publications.

  • Rahimi G  et al. A combination of herbal compound (SPTC) along with exercise or metformin more efficiently alleviated diabetic complications through down-regulation of stress oxidative pathway upon activating Nrf2-Keap1 axis in AGE rich diet-induced type 2 diabetic mice. Nutr Metab (Lond) 18:14 (2021). PubMed: 33468193
  • Montt-Guevara MM  et al. D-Chiro-Inositol Regulates Insulin Signaling in Human Adipocytes. Front Endocrinol (Lausanne) 12:660815 (2021). PubMed: 33859622
  • Zhuo J  et al. Evaluation of type 2 diabetic mellitus animal models via interactions between insulin and mitogen-activated protein kinase signaling pathways induced by a high fat and sugar diet and streptozotocin. Mol Med Rep 17:5132-5142 (2018). PubMed: 29393432
  • Wang N  et al. Hydroxytyrosol ameliorates insulin resistance by modulating endoplasmic reticulum stress and prevents hepatic steatosis in diet-induced obesity mice. J Nutr Biochem 57:180-188 (2018). WB ; Mouse . PubMed: 29747118
  • Liu Z  et al. Adiponectin reduces ER stress-induced apoptosis through PPARa transcriptional regulation of ATF2 in mouse adipose. Cell Death Dis 7:e2487 (2016). WB ; Mouse . PubMed: 27882945

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

1-3 of 3 Abreviews or Q&A

Immunocytochemistry/ Immunofluorescence abreview for Anti-Glucose Transporter GLUT4 (phospho S488) antibody [EPR930(2)]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Baboon Cell (fetal skeletal muscle)
Permeabilization
No
Specification
fetal skeletal muscle
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Formaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

DR. Soon-Ok Kim

Verified customer

Submitted Oct 02 2017

Western blot abreview for Anti-Glucose Transporter GLUT4 (phospho S488) antibody [EPR930(2)]

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Loading amount
50 µg
Gel Running Conditions
Reduced Denaturing (12.5% gel)
Sample
Chinese Hamster Cell lysate - whole cell (ovary)
Specification
ovary
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Daniel Wang

Verified customer

Submitted Feb 24 2015

Western blot abreview for Anti-Glucose Transporter GLUT4 (phospho S488) antibody [EPR930(2)]

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Loading amount
50 µg
Gel Running Conditions
Reduced Denaturing (10%, No heat)
Sample
Baboon Tissue lysate - other (Fetal skeletal muscle)
Specification
Fetal skeletal muscle
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

DR. Soon-Ok Kim

Verified customer

Submitted Dec 12 2014

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